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  4. Human Vascular Endothelial cell Growth Factor B,VEGF-B ELISA KIT

Human Vascular Endothelial cell Growth Factor B,VEGF-B ELISA KIT

文献引用 (1) 说明书下载
  • 中文名称：
    人血管内皮细胞生长因子B(VEGF-B)酶联免疫试剂盒
  • 货号：
    CSB-E04758h
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO人血管内皮细胞生长因子B（VEGF-B）酶联免疫检测试剂盒（货号CSB-E04758h），采用双抗体夹心法原理实现定量检测，适用于科研领域中对血清、血浆及组织匀浆样本中VEGF-B蛋白水平的精准分析。VEGF-B作为血管内皮生长因子家族成员，在血管生成调控、代谢稳态维持及神经保护等生理病理过程中发挥重要作用。该试剂盒检测范围覆盖15.6-1000 pg/mL，最低检测灵敏度达15.6 pg/mL，内置预包被特异性捕获抗体的96孔板、生物素标记检测抗体及系列浓度标准品，配套提供样品稀释液、显色底物和终止液等完整组分，所有组分均经过严格验证，可在4℃或-20℃条件下稳定保存6个月。实验全程约需4.5小时，包含标准品梯度稀释和样本预处理步骤，操作流程标准化且支持多指标平行检测。该试剂盒广泛应用于心血管疾病机制研究、代谢相关疾病模型评估、神经退行性病变干预实验等科研场景，尤其适用于探究VEGF-B在组织修复、能量代谢调控及疾病微环境中的作用机制，为体外实验提供可靠的蛋白定量解决方案。
  • 别名：
    Vascular endothelial growth factor B ELISA Kit; Vascular endothelial growth factor-related factor ELISA Kit; VEGF B ELISA Kit; VEGF related factor ELISA Kit; VEGF-B ELISA Kit; VEGF-related factor ELISA Kit; Vegfb ELISA Kit; VEGFB_HUMAN ELISA Kit; VEGFL ELISA Kit; VRF ELISA Kit
  • 缩写：
    VEGFB
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human VEGF-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %88
    Range %80-96
    1:2Average %100
    Range %95-104
    1:4Average %99
    Range %92-110
    1:8Average %89
    Range %86-96
  • 回收率：
    The recovery of human VEGF-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9995-105
    EDTA plasma (n=4)9692-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.697 2.512 2.605 2.463
    5002.322 2.201 2.262 2.120
    2501.546 1.498 1.522 1.380
    1250.815 0.789 0.802 0.660
    62.50.417 0.410 0.414 0.272
    31.20.281 0.276 0.279 0.137
    15.60.219 0.213 0.216 0.074
    00.143 0.140 0.142 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

产品评价

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VEGFB antibodies

VEGFB Proteins

靶点详情

  • 功能：
    Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.
  • 基因功能参考文献：
    1. High plasma VEGF-B levels are associated with type 2 diabetes mellitus. PMID: 28523459
    2. Data from clinical studies point out the changes in circulating or tissue expression levels of VEGF-B in obese compared with lean patients. PMID: 28798193
    3. Cardiac transgenic vascular endothelial growth factor-B overexpression failed to protect heart transplants from ischemia-reperfusion injury. PMID: 27588416
    4. renal VEGF-B expression correlates with the severity of Diabetic Kidney Disease. PMID: 28190774
    5. Data show that metformin treatment reduces serum vascular endothelial growth factor B (VEGF-B) levels and ameliorates insulin resistance. PMID: 26387747
    6. Frameshift mutations of VEGFB gene is associated with stomach and colorectal cancers. PMID: 25633991
    7. fluid shear stress induces the synthesis of Insulin growth factor-2 and vascular endothelial growth factor (VEGF) B and D, which in turn transactivate MMP-12. PMID: 25435370
    8. MMP9 may activate VEGF-B via PI3K/Akt signaling pathway. PMID: 25424698
    9. Roles of vascular endothelial growth factor in amyotrophic lateral sclerosis. PMID: 24987705
    10. Low VEGFB and VEGFD gene expression is associated with early-stage non-small cell lung cancer. PMID: 24145997
    11. Our study suggested that VEGF-B was an angiogenesis factor in vitro and that ERK1/2 and p38-related signaling pathways were involved in these VEGF-B activities. PMID: 24374930
    12. VEGF-B has possible roles in cardiac protection, energy metabolism support, and neuroprotectin [review] PMID: 24987005
    13. High VEGF-B levels might correlate with the presence of hyperlipidemia and target organ damage in type 2 diabetic patients. PMID: 25001655
    14. High VEGFB expression is associated with bone marrow metastasis in neuroblastoma. PMID: 23553333
    15. VEGF-B might be an important ligand in the signalling between the tumor and preexisting blood vessels to ensure a functional blood supply for tumor survival. PMID: 23417498
    16. we report significant associations with overall survival and distant failure for certain VEGF(VEGF-B) family members. PMID: 23728940
    17. Data indicate that three miRs (miR-484, -642, and -217) were able to predict chemoresistance and vasculature of serous epithelial ovarian carcinomas through the regulation of the VEGFB and VEGFR2 pathways. PMID: 23697367
    18. Expression of VEGF-B genes in glioma cell lines U87 is significantly changed under hypoxia and ischemic conditions. PMID: 23350126
    19. In WT1 mutant cells, reduced VEGF(165)b was due to lack of WT1-mediated transcriptional repression of the splicing-factor kinase SRPK1 PMID: 22172722
    20. analysis of binding of vascular endothelial growth factor-B by VEGFR-1(D2) PMID: 20501651
    21. TIMP3 blocks the binding of VEGF to VEGF receptor-2 and inhibits downstream signaling and angiogenesis. PMID: 12652295
    22. Results describe the crystal structure of human vascular endothelial growth factor-B (VEGF-B) and present a predicted model for the association of VEGF-B with the second domain of its receptor, VEGFR-1. PMID: 16616187
    23. Basophils could play a role in angiogenesis and inflammation through the expression of several forms of VEGF-B and their receptors. PMID: 17082651
    24. VEGFB, and receptor were highly expressed in dysplastic neurons. IR in astroglial and balloon cells was observed for VEGFA and its receptors Double-labeling also showed expression of VEGFA, VEGFB and VEGFR-1 in cells of the microglia/macrophage lineage. PMID: 18317782
    25. VEGF-B appears to have a relatively restricted angiogenic activity in the ischemic heart. PMID: 18511699
    26. Increased VEGFB expression is associated with hepatocellular carcinoma PMID: 18537151
    27. Overexpression of vascular endothelial growth factor-B in mouse heart alters cardiac lipid metabolism and induces myocardial hypertrophy. PMID: 18757827
    28. The structural features of the 'highly ordered' interaction of the Fab fragment of the antibody (Fab-2H10) with VEGF-B, is presented. PMID: 18930733
    29. VEGF-B mRNA was not expressed either in normal urothelium or in bladder cancer. PMID: 19424629

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  • 亚细胞定位：
    Secreted. Note=Secreted but remains associated to cells or to the extracellular matrix unless released by heparin.
  • 蛋白家族：
    PDGF/VEGF growth factor family
  • 组织特异性：
    Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas.
  • 数据库链接：

    HGNC: 12681

    OMIM: 601398

    KEGG: hsa:7423

    STRING: 9606.ENSP00000311127

    UniGene: Hs.732095

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