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中文名称:人斑联蛋白(ZYX)酶联免疫试剂盒
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货号:CSB-EL027165HU
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规格:96T/48T
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价格:¥3600/¥2500
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其他:
产品详情
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产品描述:CUSABIO人斑联蛋白(ZYX)酶联免疫吸附检测试剂盒(货号CSB-EL027165HU),采用双抗体夹心法定量检测血清、血浆、组织匀浆或细胞裂解液中的ZYX蛋白浓度。斑联蛋白(Zyxin)是细胞黏着斑的关键成分,参与细胞骨架与细胞外基质间的动态连接,在细胞迁移、机械信号传导及黏附调控中发挥重要作用。本试剂盒检测灵敏度为23.5 pg/mL,线性检测范围为23.5-1500 pg/mL,通过预包被特异性捕获抗体与标记检测抗体的协同作用实现精准定量,配套提供标准品、显色底物及终止液等完整组分。适用于细胞黏附机制研究、肿瘤迁移侵袭模型分析、心血管疾病相关细胞力学响应实验,以及组织工程或再生医学领域的细胞外基质相互作用研究等科研场景。该试剂盒支持多种生物样本类型检测,操作流程标准化,可在常规实验室条件下完成目标蛋白的定量分析,为探索ZYX在细胞行为调控及病理生理过程中的功能提供可靠工具。
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别名:ZYX ELISA Kit; Zyxin ELISA Kit; Zyxin-2 ELISA Kit
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缩写:
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Uniprot No.:
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种属:Homo sapiens (Human)
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样本类型:serum, plasma, tissue homogenates, cell lysates
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检测范围:23.5 pg/mL-1500 pg/mL
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灵敏度:5.8 pg/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Epigenetics and Nuclear Signaling
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of Human ZYX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 88 Range % 84-94 1:2 Average % 94 Range % 90-98 1:4 Average % 92 Range % 85-96 1:8 Average % 91 Range % 88-95 -
回收率:
The recovery of Human ZYX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 93 89-97 EDTA plasma (n=4) 88 84-94 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 1500 2.813 2.618 2.716 2.611 750 2.318 2.414 2.366 2.261 375 1.825 1.722 1.774 1.669 187.5 0.984 0.968 0.976 0.871 94 0.533 0.567 0.550 0.445 47 0.301 0.322 0.312 0.207 23.5 0.192 0.185 0.189 0.084 0 0.104 0.105 0.105 -
数据处理:
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货期:3-5 working days
引用文献
- Identification of a Novel Serum Biomarker for Tuberculosis Infection in Chinese HIV Patients by iTRAQ-Based Quantitative Proteomics Chen C,Frontiers in microbiology,2023
- Quantitative proteomic analysis of human serum using tandem mass tags to predict cardiovascular risks in patients with psoriasis NY Kim,Scientific reports,2023
- Zyxin expression levels in non-small cell lung cancer patients Ilic D, et al,Vojnosanitetski Pregled,2019
相关产品
靶点详情
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功能:Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression.
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基因功能参考文献:
- Study findings identify zyxin as a physiological regulator of endothelial exocytosis through reorganizing local actin network in the final stage of exocytosis. Zyxin downregulation inhibits the secretion of VWF from human primary endothelial cells. PMID: 28256511
- these observations suggest that Zyxin promotes colon cancer tumorigenesis in a mitotic-phosphorylation-dependent manner and through CDK8-mediated YAP activation. PMID: 29967145
- Increased zyxin expression is associated with asthma. PMID: 28278518
- Zyxin binding to Ptc2 is due to the interaction of Zyxin 2nd LIM-domain (530-590 aa) with the under-membrane region of the cytoplasmic C-terminus of Ptc2 (1159-1412 aa). PMID: 27125030
- ZYX gene may be aging-related gene and involved in Osteoporosis. PMID: 25771989
- key regulator of stretch-induced gene expression at the cellular level PMID: 26071033
- Matrix density alters zyxin phosphorylation, which limits peripheral process formation and extension in endothelial cells invading 3D collagen matrices. PMID: 25038525
- Zyxin, which is thought to be essential for tight cell-to-cell junctions, decreased 1.8-fold in TNF-alpha-treated human brain microvascular endothelial cells. PMID: 25025691
- Integrin-beta5 and zyxin mediate formation of ventral stress fibers in response to transforming growth factor beta. PMID: 24036928
- Zyxin is a novel target for beta-amyloid peptide activities in Alzheimer's disease. PMID: 23330981
- Zyxin is suggested to promote growth, migration and invasiveness of fibroblastic type oral squamous cell carcinoma cells by upregulating Rac1 and Cdc42. PMID: 23292068
- A stretch-induced signaling pathway in vascular cells leads to the activation of zyxin, a cytoskeletal protein specifically involved in transducing mechanical stimuli. PMID: 23233529
- RA-induced cancer cell cytotoxicity was significantly impaired by Zyxin or PTOV1. Overall, our findings suggest that Zyxin and PTOV1 should be considered as critical determinants in cancer therapy with retinoids PMID: 23321499
- Zyxin is a transforming growth factor-beta (TGF-beta)/Smad3 target gene that regulates lung cancer cell motility via integrin alpha5beta1. PMID: 22778267
- Zyxin phenotype is mediated by partners alpha-actinin and p130Cas and ensures that motile cells in a three-dimensional matrix explore the largest space possible in minimum time. PMID: 22395610
- Depletion of nectin-2 from L cells expressing E-cadherin results in a loss of zyxin localization to cell-cell contacts, demonstrating that the zyxin-nectin interaction plays a critical role in zyxin targeting to these sites. PMID: 22056563
- LIM domain of zyxin recruits zyxin to force-bearing sites at the leading edge of migrating cells PMID: 21889443
- Findings suggest an unforeseen role for Zyxin in DNA damage-induced cell fate control through modulating the HIPK2-p53 signaling axis. PMID: 21248071
- Zyxin, whose expression is negatively regulated by microRNA-16 (miR-16), is confirmed to be a direct target gene of miR-16. Overexpression of zyxin could also restrain cell movement and enhance cell-cell adhesion. PMID: 21360639
- Stretch appears to be an important factor in the development of endothelial dysfunction with zyxin as a potential therapeutic target to interfere with these early changes in endothelial cell phenotype PMID: 20705921
- The LIM domain protein zyxin was identified as an interaction partner for human synemin. PMID: 19853601
- This study establishes a crucial role of zyxin in thrombin signaling in endothelial cells and provides evidence for a novel PAR-1 signaling pathway mediated by zyxin. PMID: 19690217
- LIM-nebulette, Lasp-1, and zyxin may play an important role in the organization of focal adhesions PMID: 15004028
- Within Ewing tumor cells, zyxin is expressed at very low levels and remains diffusely distributed throughout the cytoplasm, instead of concentrating in actin-rich dynamic structures. Zyxin has tumor suppressor activity in Ewing tumor cells. PMID: 15748890
- zyxin indirectly enables the interaction of ZNF384/CIZ/NMP4 with p130CAS PMID: 16510139
- A sarch for genes upregulated by thymosin beta4 identified zyxin in SiHA cells in the presence of exogenously added thymosin beta4 and when thymosin beta4 is overexpressed using adenoviral vectors. PMID: 16956606
- These results suggest that zyxin is involved in mechanical-force-dependent facilitation of actin polymerization at focal adhesions. PMID: 18682496
- We identified zyxin as an interactor with endoglin in human umbilical vein vascular endothelial cells and this interaction is localized within the 47-amino acid carboxyl-terminal cytosolic domain of endoglin, and maps within zyxin residues 326-572. PMID: 15084601
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亚细胞定位:Cytoplasm. Cytoplasm, cytoskeleton. Nucleus. Cell junction, focal adhesion. Note=Associates with the actin cytoskeleton near the adhesion plaques. Enters the nucleus in the presence of HESX1.
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蛋白家族:Zyxin/ajuba family
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数据库链接: