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中文名称:人膜内氨酰氨基肽酶(ANPEP)酶联免疫试剂盒
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货号:CSB-EL001827HU
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规格:96T/48T
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价格:¥3600/¥2500
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其他:
产品详情
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产品描述:本试剂盒为人膜内氨酰氨基肽酶(ANPEP)定量检测提供高效解决方案,采用双抗体夹心ELISA技术原理,可精准测定血清、血浆及组织匀浆样本中的ANPEP蛋白水平。ANPEP作为一种跨膜锌依赖性蛋白酶,广泛参与细胞黏附、肽类激素代谢及免疫调节等生物学过程,其表达水平与肿瘤转移、炎症反应及心血管疾病发生发展密切相关。试剂盒标准检测范围为31.25-2000 pg/mL,通过预包被特异性捕获抗体与酶标检测抗体的协同作用,实现目标蛋白的高灵敏度定量分析,最低检测限达15.6 pg/mL。该产品适用于科研领域中对ANPEP表达调控机制的研究,如肿瘤微环境分析、免疫细胞功能探索或代谢相关疾病模型构建等应用场景,尤其适用于需要同时检测体液样本(血清/血浆)和组织样本的研究设计。试剂盒提供优化的预包被板条及即用型检测试剂,配套标准品和生物素化检测抗体,确保实验流程简便且结果稳定可靠,为细胞生物学、免疫学及疾病机制研究提供有力工具。
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别名:Alanyl (membrane) aminopeptidase ELISA Kit; Alanyl aminopeptidase ELISA Kit; Aminopeptidase M ELISA Kit; Aminopeptidase N ELISA Kit; AMPN_HUMAN ELISA Kit; ANPEP ELISA Kit; AP M ELISA Kit; AP N ELISA Kit; AP-M ELISA Kit; AP-N ELISA Kit; APN ELISA Kit; CD 13 ELISA Kit; CD13 ELISA Kit; CD13 antigen ELISA Kit; gp150 ELISA Kit; hAPN ELISA Kit; LAP 1 ELISA Kit; LAP1 ELISA Kit; Microsomal aminopeptidase ELISA Kit; Myeloid plasma membrane glycoprotein CD13 ELISA Kit; p150 ELISA Kit; PEPN ELISA Kit
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缩写:
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Uniprot No.:
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种属:Homo sapiens (Human)
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样本类型:serum, plasma, tissue homogenates
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检测范围:31.25 pg/mL-2000 pg/mL
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灵敏度:7.81 pg/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Cardiovascular
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human ANPEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:20 Average % 91 Range % 84-99 1:40 Average % 91 Range % 85-96 1:80 Average % 96 Range % 92-103 1:160 Average % 103 Range % 96-109 -
回收率:
The recovery of human ANPEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 92 88-97 EDTA plasma (n=4) 96 90-102 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 2000 2.627 2.645 2.636 2.545 1000 2.131 2.298 2.215 2.124 500 1.458 1.502 1.480 1.389 250 0.731 0.755 0.743 0.652 125 0.377 0.352 0.365 0.274 62.5 0.201 0.212 0.207 0.116 31.25 0.189 0.188 0.189 0.098 0 0.087 0.094 0.091 -
数据处理:
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货期:3-5 working days
引用文献
- Correlation of serum adipocytokine levels with glycolipid metabolism and inflammatory factors in obese patients with periodontal disease Zejian Li.et al,Int J Clin Exp Pathol,2018
相关产品
靶点详情
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功能:Broad specificity aminopeptidase which plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. May also be involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. May have a role in angiogenesis and promote cholesterol crystallization. May have a role in amino acid transport by acting as binding partner of amino acid transporter SLC6A19 and regulating its activity.; (Microbial infection) Acts as a receptor for human coronavirus 229E/HCoV-229E. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein.; (Microbial infection) Mediates as well Human cytomegalovirus (HCMV) infection.
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基因功能参考文献:
- Crosslinking of CD13 by mAb C or E is required to inhibit adhesion, as monovalent Fab fragments are not sufficient. Thus, C and E antibodies recognize a distinct epitope on CD13, and binding to this epitope interferes with both CD13-mediated cell adhesion and enzymatic activity. PMID: 29789790
- Data indicate the expression of aminopeptidase N (CD13) in small cell lung cancer (SCLC) tumor and suggest pre-therapeutic CD13 analysis for testing as investigational predictive biomarker for patient selection. PMID: 29110838
- CD13 may be a marker for onychofibroblasts within nail matrix onychodermis PMID: 28708295
- This is the first report presenting CD13 and FLI1 as important mediators of resistance to BRAF inhibition with potential as drug targets in BRAF inhibitors refractory melanoma. PMID: 29048432
- CD13 enrichment was correlated with early recurrences, and poor prognosis in patients with hepatocellular carcinoma. PMID: 29145632
- Patients with acute myeloid leukemia developing leukemia-specific acute hypoxic respiratory failure within 15 days had higher CD13 expression by leukemic cells. PMID: 28323433
- Data suggest that CNGRC-GG-D(KLAKLAK)2 peptide actively participates in the downregulation of aminopeptidase N/CD13. PMID: 26655501
- CGRP and IL-4 positively regulated APN/CD13 expression and activity in psoriatic fibroblasts. PMID: 28387421
- this study shows that CD13 significantly contributes to tissue infiltration by monocytic myeloid-derived suppressor cells and monocytes, thereby contributing to the pathogenesis of hepatic inflammation PMID: 28739875
- data reveal a novel role for CD13 in inducing homotypic aggregation in neutrophils, which results in a transmigration deficiency; this mechanism may be relevant to neutrophil micro-aggregation in vivo PMID: 27467268
- CD13 expression is associated with hepatoblastoma invasiveness and could be a novel prognostic marker for hepatoblastoma. PMID: 25682862
- Data indicate that fluorogenic substrates can be successfully used to identify aminopeptidase N and to measure their activity in cell lysates. PMID: 26449746
- Data show that CD13 anntigen and receptor tyrosine kinase-like orphan receptor 2 (ROR2) identify a cardiac lineage precursor pool that is capable of successful engraftment into the porcine heart. PMID: 26771355
- The substrate Angiotensin II, the enzymes aminopeptidases-A, B, M as well as IRAP were detected in the jejunal mucosa. PMID: 26311161
- 14-3-3epsilon might directly bind to CD13, which transmits its signal in chondrocytes to induce a catabolic phenotype similar to that observed in osteoarthritis. PMID: 26208633
- Alanyl aminopeptidase expression is confined to mature zymogenic chief cells and its expression is lost en route to metaplasia. PMID: 26514774
- Aminopeptidase N activity in tissue and plasma from colorectal cancer patients is an independent prognostic factor of 5-year survival. PMID: 25929234
- Expression of APN/CD13 is a potential unfavorable factor to predict the efficacy and prognosis of post-operative chemotherapy in NSCLC patients, especially in lung adenocarcinoma patients. PMID: 25879366
- We identified a unique HCC line, Li-7, which not only shows heterogeneity for a CD13(+) CSC hierarchy, but also undergoes a "population change" upon CSC differentiation PMID: 25885470
- This study permitted the identification of the novel human LAP1C isoform and partially unraveled the molecular basis of LAP1 regulation. PMID: 25461922
- Together these experiments reveal potential enzymatic and signalling roles for APN in osteosarcoma and establish a starting point for an in-depth analysis of the role of APN in regulating invasiveness. PMID: 25340499
- Postulate that the interaction of ADAM17 with CD13 and its downregulation following CD13 engagement has important implications in acute myeloid leukemia cells. PMID: 25246708
- CD13 could play an important role as a T cell chemoattractant, in a positive feedback loop that contributes to rheumatoid arthritis synovitis. PMID: 25219368
- This study showed that CD13 and CD33 expression associated with poor prognosis in patients with MM implicating the need of analysis of these markers in MM diagnosis. PMID: 24991573
- Aberrant expression of CD13 in >/= 5% of blasts of patients with SR-aALL is an adverse prognostic factor, delineating a subgroup of patients with SR-aALL that should be considered for more aggressive treatment. PMID: 24411984
- Molecular mechanisms regulating CD13-mediated adhesion. PMID: 24627994
- CD13 participates in phagocytic processes in dendritic cells and macrophages. PMID: 24063007
- Antigen CD13, by influencing the adipogenic potential of human amniotic mesenchymal stem cells, could be an in utero risk factor for obesity. PMID: 23488598
- NGR-LDP could bind to CD13-expressing HT-1080 cells. PMID: 23206754
- results indicate that APN/CD13 could be an important diagnostic biomarker and therapeutic target for Malignant fibrous histiocytoma . PMID: 23677132
- Activities of aminopeptidases N and B and insulin-regulated aminopeptidase could be useful non-invasive biomarkers of Alzheimer's disease from the earliest stages. PMID: 23500679
- High CD13 expression is associated with Philadelphia chromosome negative B cell acute lymphoblastic leukemia. PMID: 23643324
- results showed that ANPEP downregulation in prostate cancer (PC) was frequently associated with aberrant promoter hypermethylation, suggesting that epigenetic mechanisms are involved in silencing of ANPEP in PC cells PMID: 23322201
- CD13 expression plays a role in supporting the survival of cancer stem cells and that there is an epithelial-mesenchymal transition-associated reduction in reactive oxygen species elevation. PMID: 21879266
- The hAPN crystal structure provides the first example of a dimeric M1 family member and the observed structural features provide novel insights into the mechanism of peptide processing and signal transduction. PMID: 22932899
- Inhibiting CD13 with bestatin may enhance the differentiation-inducing activity of all-trans-retinoic acid in NB4 cells. PMID: 22040956
- Coexpression of APN/CD13 correlated significantly with tumor type and neoangiogenesis in breast cancer. PMID: 21611838
- Myeloid cell surface peptidase CD13 is highly and specifically expressed on a subset of dendritic cells (DCs) responsible for cross-presentation: transgenic CD8+ murine splenic DCs. PMID: 22544935
- The overall effects of APN on the expression of fibronectin, tenascin-C, and MMPs in fibroblasts propose an important role for APN in the regulation of keratinocyte-mediated ECM remodeling and fibroblast contractile activity. PMID: 22065384
- Staining of tissue microarrays generated from a large series of melanoma samples and control tissues demonstrated a higher APN expression in primary melanoma lesions when compared with nevi and metastases PMID: 22307972
- A peptide-targeted gene vector for highly efficient receptor-mediated intracellular delivery is used to target gene loaded poly(lactic acid)-poly(ethylene glycol) nanoparticles to vascular endothelial cells overexpressing CD13. PMID: 21376765
- Murine gamma-herpesvirus-68 glycoprotein (gp)150-specific T-cell receptor epitope transgene activates antiviral CD4+ T cells that are maintained throughout long-term infection and even during quiescent latency. PMID: 22079983
- We identified a variant in each of 3 genes (RFC1, SCN1A, ANPEP) potentially implicated in susceptibility to severe neurological complications in West Nile Virus disease. PMID: 21881118
- APN expression and enzymatic function is reduced in aggressive meningiomas, and that alterations in the balance between APN and SPARC might favor meningioma invasion PMID: 19236378
- analysis of the critical role of flanking residues in NGR-to-isoDGR transition and CD13/integrin receptor switching PMID: 20064928
- Stromal aminopeptidase N expression correlates with angiogenesis in non-small-cell lung cancer. PMID: 19908113
- data demonstrate that the glycosylation of aminopeptidase N at N291 blocks human coronavirus-229E infection PMID: 11774469
- tested the hypothesis that CD13 influences proliferation of monocytoid cells by retarding the velocity of the cell cycle PMID: 11999577
- CD13/APN is an important target of Ras signaling in angiogenesis and is a limiting factor in angiogenic progression. PMID: 12406907
- inhibiting CD13/aminopeptidase N on the cell-surface of acute promyelocytic leukemia cells increases ATRA-induced differentiation PMID: 12443882
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亚细胞定位:Cell membrane; Single-pass type II membrane protein.
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蛋白家族:Peptidase M1 family
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组织特异性:Expressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial st
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