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  4. Human cytokeratin 18,CK-18 ELISA Kit

Human cytokeratin 18,CK-18 ELISA Kit

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  • 中文名称：
    人细胞角蛋白18(CK-18)酶联免疫试剂盒
  • 货号：
    CSB-E09172h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO试剂盒采用双抗体夹心酶联免疫吸附法，专为定量检测人血清、血浆、组织匀浆、细胞裂解液及细胞培养上清中的细胞角蛋白18（CK-18）而设计。CK-18是上皮细胞特异性中间丝蛋白，在细胞损伤或凋亡时释放至胞外，其水平变化与上皮源性肿瘤、肝纤维化等病理过程密切相关，是研究细胞应激和疾病机制的重要生物标志物。试剂盒检测范围覆盖0.625至15 ng/mL，灵敏度高且特异性强，适用于多种生物样本分析，可满足体外药物毒性评估、肿瘤微环境研究或组织损伤模型的科研需求。操作流程标准化，提供预包被96孔板及完整检测组分，支持科研人员高效开展上皮相关疾病的分子机制探索或生物标志物筛选工作，所有实验数据需严格用于科学研究用途。
  • 别名：
    Cell proliferation inducing gene 46 protein ELISA Kit; Cell proliferation inducing protein 46 ELISA Kit; Cell proliferation-inducing gene 46 protein ELISA Kit; CK 18 ELISA Kit; CK-18 ELISA Kit; CK18 ELISA Kit; CYK 18 ELISA Kit; CYK18 ELISA Kit; Cytokeratin 18 ELISA Kit; Cytokeratin endo B ELISA Kit; Cytokeratin-18 ELISA Kit; K 18 ELISA Kit; K18 ELISA Kit; K1C18_HUMAN ELISA Kit; KA18 ELISA Kit; Keratin 18 ELISA Kit; Keratin 18, type I ELISA Kit; Keratin D ELISA Kit; keratin, type I cytoskeletal 18 ELISA Kit; Keratin-18 ELISA Kit; Krt18 ELISA Kit
  • 缩写：
    KRT18
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates, cell culture supernates
  • 检测范围：
    0.625 ng/mL-15 ng/mL
  • 灵敏度：
    0.25 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<10%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CK-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 90-100  
    1:2 Average % 92  
    Range % 88-99  
    1:4 Average % 97  
    Range % 92-106  
    1:8 Average % 94  
    Range % 86-103  
  • 回收率：
    The recovery of human CK-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 89-102  
    EDTA plasma (n=4) 97 92-105  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    15 1.932 1.958 1.945 1.898  
    7.5 1.318 1.357 1.338 1.291  
    3.75 0.786 0.798 0.792 0.745  
    1.25 0.375 0.362 0.369 0.322  
    0.625 0.216 0.223 0.220 0.173  
    0 0.046 0.047 0.047 　  
  • 本试剂盒所含材料：
    • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-glucagon antibody.
    • Six vials standard (5ml/bottle)--- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial HRP-conjugated glucagon antibody (6 ml/bottle) --- Bind to the glucagon in the samples or standards and react with the substrate to make the solution chromogenic.
    • One vial Wash Buffer (20x concentrate) (15 ml/bottle) --- Wash away unbound or free substances.
    • One vial Substrate A (7ml/bottle) --- Mix with substrate B and then interact with TMB to elicit a chromogenic reaction.
    • One vial Substrate B (7ml/bottle) --- Mix with substrate A and interact with TMB to elicit a chromogenic reaction.
    • One vial Stop Solution (7ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

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  • 本试剂盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm or 630 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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KRT18 antibodies

KRT18 Proteins

相关问答

■ 常见问题解答
Q:

Could you please advice if you supply the quality control for this kit?Hope to hear from you soon.I am referring to the high and low control. Is it included in the kit?

A:
Thanks for your inquiry. Sorry the kit does not include the mentioned control.But it contains the standard in it which is used to validate and analyze the whole reagent system and can work as controls.

靶点详情

  • 功能：
    Involved in the uptake of thrombin-antithrombin complexes by hepatic cells. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
  • 基因功能参考文献：
    1. the present study demonstrated that CK18 might be served as a novel biomarker to predict clinicopathological features and the outcome of breast cancer. PMID: 29437899
    2. This study analyzed phase III data comparing basal insulin peglispro (BIL) and insulin glargine in type 1 (T1D), and type 2 diabetes (T2D) (insulin-naive and insulin-treated). Alanine aminotransferase (ALT), K-18, enhanced liver fibrosis scores and liver fat content. PMID: 29167192
    3. Results found that CK18, MMP-9, and TIMP1 averages of those with positive clinical lymph nodes and those in clinical stage 3 were higher than the averages of those with negative clinical lymph nodes and those in clinical stage 2. PMID: 29651326
    4. non-surviving severe malignant middle cerebral artery infarction patients had higher serum CCCK-18 levels than surviving patients PMID: 29573748
    5. Plasma M30-M65 levels are higher in serum of patients with placental abruption. PMID: 27893299
    6. results show that the caspase digestion-resistant K18 helps to maintain keratin filament organization and delays apoptosis, thereby resulting in protection from liver injury. PMID: 28606991
    7. The plasma keratin-18 (K18) values of nonsurviving alcoholic hepatitis (AH) patients were significantly elevated above their surviving counterparts and healthy controls PMID: 28770701
    8. Evidence suggested that the modification of histone H3 was highly correlated with the modulation of cytokeratin 18 and probably plays an important role in tumorigenesis of hepatocytes PMID: 28647696
    9. K8/K18 interact with Notch1 and regulate Notch1 signalling activity during differentiation of the colonic epithelium. PMID: 28475172
    10. FIB-4 index and CK-18F have good diagnostic abilities not only for nonalcoholic steatohepatitis (NASH) overall, but also for NASH with mild fibrosis. PMID: 29040984
    11. In children with nonalcoholic fatty liver disease, CK18 levels were found to be significantly higher in subjects with any fibrosis compared with those without fibrosis (304.6 +/- 124.8 vs 210.4 +/- 70.9, P < 0.001). PMID: 26835904
    12. CK18 combined with uric acid measurement is a promising non-invasive biomarker for prediction of disease severity in NASH patients PMID: 28472039
    13. Results suggested that serum M65 is a useful indicator of liver inflammation in chronic hepatitis B patients. Serum M65, not M30, is valuable in the grading of liver fibrosis. PMID: 28514295
    14. The results suggest that increased circulating CK-18 and FGF-21 are associated with Nonalcoholic Fatty Liver Disease and may be used for initial assessment, but not enough PMID: 28326329
    15. The purpose of this study is to investigate the immunohistochemical expression of cytokeratin 18 (CK18) and the reactivity to GDF5 (CDMP-1), called the morphogenetic protein-1, cartilage-derived, in lingual squamous cell carcinoma. PMID: 27151703
    16. In patients with anterior STEMI treated with primary PCI, the apoptosis marker M30 might be useful for predicting LV remodeling and subsequent LV systolic dysfunction. PMID: 27479531
    17. Serum concentrations of CK-18 fragments and transgelin-2 correlate with the severity of NAFLD, but not with obesity. PMID: 27740519
    18. High KRT18 serum levels are associated with non-small-cell lung cancer. PMID: 27468866
    19. Serum HGF and CK18 levels were significantly up-regulated and positively correlated with metastasis stage, tumor stage, and disease stage of esophageal squamous cell carcinoma. PMID: 27706656
    20. CK18 plays an important role in lung cancer progression and may be a therapeutic target for non small cell lung cancer. PMID: 27601168
    21. these results suggest that cytokeratin 18 critically contributes to initiating TGF-beta1-induced EMT via the smad 2/3-mediated regulation of snail and slug expression in breast epithelial cells. PMID: 27734227
    22. Study demonstrated that the downregulation of CK18 is associated with the acquisition of paclitaxel-resistance and tumor aggressiveness in prostate cancer. PMID: 26892177
    23. Data indicate that O-GlcNAcylation at serine Ser30 determines the occurrence of Pposphorylation at Ser33 on Keratin 18 (K18). PMID: 27059955
    24. Data show that adipose-derived stem cells (ADSC) differentiate into epithelial-like cells with the expression of epithelial surface marks cytokeratin 18 and 19 and form tube-like structures. PMID: 26416346
    25. High circulating CCCK-18 levels were associated with injury severity and a poor clinical outcome after aSAH and CCCK-18 had the potential to be a good prognostic biomarker for aSAH. PMID: 26671131
    26. NAFLD patients, serum CK-18 levels reflect disease activity scores and correlate with histological changes. PMID: 26436355
    27. Data show that the decrease of keratin 18 (K18) ser33 and ser52 phosphorylation inhibited autophagy and decreased apoptosis of HCT116 cells. PMID: 26728375
    28. As an apoptosis-related biomarker, serum M30 [M30 cytokeratin-18 peptide, human ] level has a merit to be the most promising test for prediction or differential diagnosis of HELLP syndrome in PE patients. PMID: 26138305
    29. Serum caspase-cleaved cytokeratin-18 levels are associated with 30-day mortality and could be used as a prognostic biomarker in patients with severe traumatic brain injury. PMID: 25822281
    30. The results suggest that oblongifolin C inhibits metastasis through the induction of the expression of keratin 18 and may be useful in cancer therapy. PMID: 25973684
    31. Our results suggest that reliable detection of apoptosis via the M30 assay only works when sufficient levels of cytokeratin 18 are present in the cells. This means that the M30 assay may result in false negative results for apoptosis PMID: 25846732
    32. No evidence of association between polymorphisms in KRT18 and sporadic amyotrophic lateral sclerosis in Han Chinese PMID: 25677198
    33. Variants in highly conserved residues of K18 Asp89His (isoniazid-related) were found in patients with fatal Drug-induced liver injury. PMID: 26286715
    34. Serum caspase-cleaved cytokeratin-18 levels were associated with mortality in severe septic patients. PMID: 25290885
    35. Although only serum M65 levels were found to be of diagnostic value, neither M30 nor M65 serum levels played a prognostic role in the outcome in melanoma patients. PMID: 23812330
    36. Under the present study design propofol or sevoflurane anaesthesia did not induce apoptosis or affected the liver function as assessed by the M30, M65 markers and liver enzymes in patients undergoing mastectomy or thyroidectomy under general anaesthesia. PMID: 25579144
    37. There were no statistically significant correlations between serum M30 and M65 concentrations and clinicodemographical variables. Serum M30 and M65 concentrations were found to have a diagnostic value in nasopharyngeal cancer PMID: 25326440
    38. The broken dialog between ANXA1 and CK18 in normal breast tissues may play a critical role in breast cancer development. PMID: 25028131
    39. The distribution of CK18 in a large cohort of older people with type 2 diabetes is associated with an adverse metabolic risk factor profile. PMID: 24237940
    40. placental expression significantly increased in preeclampsia compared to normotensive pregnancies PMID: 24857367
    41. Full length and caspase cleaved cytokeratin 18 (detected as M65 and M30 antigens) are associated with prognosis in hepatitis B virus-related acute-on-chronic liver failure. PMID: 24605039
    42. KRT18 expression is directly regulated by EGR1, and contributes to decrease malignancy of non-small cell lung carcinoma. PMID: 24990820
    43. we identified four proteins with different expression in paclitaxel resistant cells, i.e., serpin B3, serpin B4, heat shock protein 27 (all three upregulated) and cytokeratin 18 (downregulated). PMID: 24898082
    44. effect between the CK-18 concentration and the severity of NAFLD was found in the T2DM patients PMID: 24667697
    45. Due to promising results of various studies, CK-18 cell death markers could be applied in clinical routine soon. PMID: 24622872
    46. Median serum concentration of M30-CK18 was higher in chronic hepatitis C patients and was associated with inflammatory activity and liver fibrosis. PMID: 23981197
    47. A new model that combines ALT, platelets, CK-18 fragments, and triglycerides was established by logistic regression among nonalcoholic fatty liver disease patients. PMID: 24324749
    48. Serum M65 has the potential as a new diagnostic parameter for hepatocellular carcinoma (HCC) and serum CD163 is a new prognostic parameter in HCC patients. PMID: 23792028
    49. M 30 serum levels increase in patients with active ulcerative colitis. PMID: 23945185
    50. These results provide an explanation for the elevated serum levels of HSP-27, HSP-70, and ccCK-18 found in COPD patients, indicating that hypoxic conditions can trigger the release of the aforementioned factors. PMID: 24660549

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  • 相关疾病：
    Cirrhosis (CIRRH)
  • 亚细胞定位：
    Cytoplasm, perinuclear region. Nucleus, nucleolus.
  • 蛋白家族：
    Intermediate filament family
  • 组织特异性：
    Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
  • 数据库链接：

    HGNC: 6430

    OMIM: 148070

    KEGG: hsa:3875

    STRING: 9606.ENSP00000373487

    UniGene: Hs.406013

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