Human hepatitis D virus(HDV) antibody(IgG) ELISA Kit
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中文名称：
人丁型肝炎(HDV)抗体(IgG)酶联免疫试剂盒
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货号：
CSB-E04809h
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规格：
96T/48T
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价格：
￥3200/￥2500
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其他：
产品详情
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产品描述：
CUSABIO丁型肝炎病毒（HDV）IgG抗体酶联免疫检测试剂盒（货号：CSB-E04809h），专为科研领域设计，适用于血清样本中HDV特异性IgG抗体的定性或半定量检测。HDV是一种依赖乙型肝炎病毒（HBV）进行复制的缺陷型RNA病毒，其IgG抗体可反映既往感染或持续感染状态，对研究病毒与宿主相互作用、感染病理机制及流行病学调查具有重要意义。CUSABIO试剂盒采用固相抗原包被技术，通过酶标二抗与样本中目标抗体的特异性结合，经显色反应实现检测，操作流程标准化，灵敏度与特异性经严格验证。检测范围请通过官方渠道查询具体参数。该产品可用于评估动物模型免疫应答、追踪感染人群抗体动态变化、筛查高危地区血清样本等科研场景，为病毒学、免疫学及公共卫生研究提供可靠工具。实验需配合标准实验室设备及规范操作，所有组分均经过稳定性测试，确保实验结果一致性。
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别名：
N/A
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缩写：
HDV Ab (IgG)
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种属：
Homo sapiens (Human)
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样本类型：
serum
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检测范围：
Request Information
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灵敏度：
Request Information
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反应时间：
1-5h
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样本体积：
50-100ul
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检测波长：
450 nm
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研究领域：
Microbiology
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精密度：
Intra-assay Precision (Precision within an assay): CV%<15% | | | |
Three samples of known concentration were tested twenty times on one plate to assess. | |
Inter-assay Precision (Precision between assays): CV%<15% | | | |
Three samples of known concentration were tested in twenty assays to assess. | | |
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标准曲线：
Test parameter | specification | test result |
Positive control | >0.30 | 0.842 |
Negative control | <0.10 | 0.051 |
Positive rate | 10，Positive | 100% |
Negative rate | 10，Negative | 100% |
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本试剂盒所含材料：
- A 96-well Assay plate --The 96-well plate has been pre-coated with HDV antigen.
- Negative Control (1 x 1ml) --Eliminate false positive
- Positive Control (1 x 1ml) --Used to evaluate the validity, stability, and comparability of experimental results.
- HRP-conjugated IgG antibody(1 x 12ml) --Bind to the anti-HDV IgG antibody, and HRP catalyzes the TMB to elicit a chromogenic reaction.
- Sample Diluent (1x 12 ml) --Dilute the sample to an appropriate concentration.
- Wash Buffer (20x concentrate) (1 x 50 ml) --Wash away unbound or free substances.
- Substrate A (1 x 6 ml) --Mix with substrate B and interact with HRP, eliciting a Chromogenic reaction.
- Substrate B (1 x 6 ml) --Mix with substrate A and interact with HRP, eliciting a Chromogenic reaction.
- Stop Solution (1 x 6ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells)--Cover the microplate when incubation.
- An Instruction manual
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本试剂盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 630 nm.
- An incubator that can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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货期：
3-5 working days
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