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Human hepatitis D virus(HDV) antibody(IgG) ELISA Kit

  • 中文名称:
    人丁型肝炎(HDV)抗体(IgG)酶联免疫试剂盒
  • 货号:
    CSB-E04809h
  • 规格:
    96T/48T
  • 价格:
    ¥3200/¥2500
  • 其他:

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  • 产品描述:
    CUSABIO丁型肝炎病毒(HDV)IgG抗体酶联免疫检测试剂盒(货号:CSB-E04809h),专为科研领域设计,适用于血清样本中HDV特异性IgG抗体的定性或半定量检测。HDV是一种依赖乙型肝炎病毒(HBV)进行复制的缺陷型RNA病毒,其IgG抗体可反映既往感染或持续感染状态,对研究病毒与宿主相互作用、感染病理机制及流行病学调查具有重要意义。CUSABIO试剂盒采用固相抗原包被技术,通过酶标二抗与样本中目标抗体的特异性结合,经显色反应实现检测,操作流程标准化,灵敏度与特异性经严格验证。检测范围请通过官方渠道查询具体参数。该产品可用于评估动物模型免疫应答、追踪感染人群抗体动态变化、筛查高危地区血清样本等科研场景,为病毒学、免疫学及公共卫生研究提供可靠工具。实验需配合标准实验室设备及规范操作,所有组分均经过稳定性测试,确保实验结果一致性。
  • 别名:
    N/A
  • 缩写:
    HDV Ab (IgG)
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum
  • 检测范围:
    Request Information
  • 灵敏度:
    Request Information
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Microbiology
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<15%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<15%

    Three samples of known concentration were tested in twenty assays to assess.

  • 标准曲线:

    Test parameter

    specification

    test result

    Positive control

    >0.30

    0.842

    Negative control

    <0.10

    0.051

    Positive rate

    10,Positive

    100%

    Negative rate

    10,Negative

    100%

  • 本试剂盒所含材料:
      • A 96-well Assay plate --The 96-well plate has been pre-coated with HDV antigen.
      • Negative Control (1 x 1ml) --Eliminate false positive
      • Positive Control (1 x 1ml) --Used to evaluate the validity, stability, and comparability of experimental results.
      • HRP-conjugated IgG antibody(1 x 12ml) --Bind to the anti-HDV IgG antibody, and HRP catalyzes the TMB to elicit a chromogenic reaction.
      • Sample Diluent (1x 12 ml) --Dilute the sample to an appropriate concentration.
      • Wash Buffer (20x concentrate) (1 x 50 ml) --Wash away unbound or free substances.
      • Substrate A (1 x 6 ml) --Mix with substrate B and interact with HRP, eliciting a Chromogenic reaction.
      • Substrate B (1 x 6 ml) --Mix with substrate A and interact with HRP, eliciting a Chromogenic reaction.
      • Stop Solution (1 x 6ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells)--Cover the microplate when incubation.
      • An Instruction manual

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  • 本试剂盒不含材料:
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 630 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 货期:
    3-5 working days

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