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中文名称：人1,4,5-三磷酸肌醇(IP3)酶联免疫试剂盒
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货号：CSB-E12636h
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规格：96T/48T
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价格：￥3600/￥2500
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其他：
产品详情
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产品描述：人1,4,5-三磷酸肌醇(IP3)酶联免疫试剂盒（CSB-E12636h）为竞争法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IP3含量。IP3即三磷酸肌醇，是细胞内重要的第二信使。其背景在于它在细胞信号转导中扮演关键角色。研究机制上，当细胞外信号激活相应受体，通过G蛋白激活磷脂酶C，水解磷脂酰肌醇生成IP3，它能促使内质网释放Ca²⁺，引发一系列细胞反应。试剂盒检测范围为5 pg/mL-1000 pg/mL，尤其适用于体外研究细胞信号通路激活程度、药物靶点作用机制或疾病模型中IP3动态变化的分析；为探究G蛋白偶联受体介导的磷酸肌醇代谢、细胞应激反应调控等基础研究提供可靠工具，广泛适配于神经生物学、药理学及病理生理学等领域的科研需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
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缩写：IP3
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种属：Homo sapiens (Human)
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样本类型：serum, plasma, cell culture supernates, tissue homogenates
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检测范围：5 pg/mL-1000 pg/mL
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灵敏度：2.5 pg/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Signal Transduction
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测定原理：quantitative
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测定方法：Competitive
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精密度：
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human IP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 95 Range % 90-105 1:2 Average % 94 Range % 89-100 1:4 Average % 94 Range % 87-102 1:8 Average % 95 Range % 90-102 -
回收率：
The recovery of human IP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 91-100 EDTA plasma (n=4) 92 87-96 -
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average 1000 0.202 0.211 0.207 250 0.350 0.349 0.350 80 0.600 0.594 0.597 20 1.029 1.018 1.024 5 1.326 1.345 1.336 0 2.102 2.130 2.116 -
数据处理：
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货期：3-5 working days
引用文献
- Second messenger role for Mg21 revealed by human T-cell immunodeficiency Feng-Yen Li,Nature,2011
- C6-ceramide induces salivary adenoid cystic carcinoma cell apoptosis via IP3R-activated UPR and UPR-independent pathways L Qiu,Biochem. Biophys. Res. Commun,2020
- High-dose levocetirizine for the treatment of refractory chronic spontaneous urticaria and the effect on the serum inositol triphosphate level Huang X, et al,Journal of International Medical Research,2019
- Activation of calcium signaling in human gingival fibroblasts by recombinant Porphyromonas gingivalis RgpB protein Li S,european journal of oral sciences,2019
- Inositol 1,4,5-trisphosphate (IP3)-dependent Ca2+ signaling mediates delayed myogenesis in Duchenne muscular dystrophy fetal muscle Farini A.et al,Development.,2016
- Combined assessment of polymorphisms in the LHCGR and FSHR genes predict chance of pregnancy after in vitro fertilization Lindgren I. et al,Hum Reprod,2016
- Cilostazol Induces PGI 2 Production via Activation of the Downstream Epac-1/Rap1 Signaling Cascade to Increase Intracellular Calcium by PLC(Epsilon) and to Activate p44/42 MAPK in Human Aortic Endothelial Cells. Hashimoto A et al,PLoS One,2015
- Octopamine Regulates Antennal Sensory Neurons via Daytime-Dependent Changes in cAMP and IP3 Levels in the Hawkmoth Manduca sexta. Schendzielorz T et al,PLoS One,2015
- Cytochrome C dysregulation induced by HIV infection of astrocytes results in bystander apoptosis of uninfected astrocytes by an IP3 and calcium-dependent mechanism Eugenin EA et al,J Neurochem,2013
- Second messenger role for Mg2+ revealed by human T-cell immunodeficiency /,Nature,2011
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相关问答
■ 常见问题解答
Q:
I would like to receive more information about this assay, particularly regarding specificity for IP3.
Please provide some more information about the following.
a. capture antibody or capture antigen
b. Detection antibody (if applicable)
c. standard – if recombinant protein please specify sequence (including species source and accession no. if available) and expression host.
Are you aware of any product specific references for this assay? If yes please can you provide links to these references. If you have any other information about validation of the antibodies in other applications e.g. WB this information would be helpful also.
A:
Thanks for your inquiry.
CSB-E12636h
This is a competitive kit.
The plate is coated with goat-anti-rabbit antibody.
The specific antibody is rabbit polyclonal antibody.
The standard is purified IP3.
The raw materials of the kit have been screened strictly and passed quality control. Sorry it is not convenient for us to provide validation information of the antibodies because it is only for our internal tracing. There are many references about this kit. Pls find following links:
1. https://www.ncbi.nlm.nih.gov/pubmed/?term=26884398 https://academic.oup.com/humrep/article/31/3/672/2384908
3. https://www.ncbi.nlm.nih.gov/pubmed/26181635
4.https://www.ncbi.nlm.nih.gov/pubmed/25785721
5. https://www.ncbi.nlm.nih.gov/pubmed/23992092
6. https://www.nature.com/articles/nature10246?WT.ec_id=NATURE-20110728
Pls let me know if you have any further questions. Thank you.
CSB-E12636h
This is a competitive kit.
The plate is coated with goat-anti-rabbit antibody.
The specific antibody is rabbit polyclonal antibody.
The standard is purified IP3.
The raw materials of the kit have been screened strictly and passed quality control. Sorry it is not convenient for us to provide validation information of the antibodies because it is only for our internal tracing. There are many references about this kit. Pls find following links:
1. https://www.ncbi.nlm.nih.gov/pubmed/?term=26884398 https://academic.oup.com/humrep/article/31/3/672/2384908
3. https://www.ncbi.nlm.nih.gov/pubmed/26181635
4.https://www.ncbi.nlm.nih.gov/pubmed/25785721
5. https://www.ncbi.nlm.nih.gov/pubmed/23992092
6. https://www.nature.com/articles/nature10246?WT.ec_id=NATURE-20110728
Pls let me know if you have any further questions. Thank you.
