-
中文名称：人白介素18结合蛋白(IL-18BP)酶联免疫试剂盒
-
货号：CSB-E13687h
-
规格：96T/48T
-
价格：￥3600/￥2500
-
其他：
产品详情
-
产品描述：人白介素18结合蛋白(IL-18BP)酶联免疫试剂盒（CSB-E13687h）为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的IL18BP含量。IL18BP即白介素18结合蛋白，它可与IL-18结合阻断其活性。IL-18参与机体免疫调节、炎症反应等。IL18BP作为内源性抑制剂，调控IL-18活性，有潜力用于炎症相关疾病如关节炎、感染性疾病的治疗研究。试剂盒检测范围为15.6 pg/ml-1000 pg/ml，灵敏度为3.9 pg/ml。适用于基础医学研究领域，例如探究炎症相关疾病的分子机制、评估免疫调节药物对IL - 18/IL - 18BP信号通路的影响，或作为生物标志物在心血管疾病、代谢综合征等病理模型中的动态监测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
-
别名：I18BP_HUMAN ELISA Kit; IL-18BP ELISA Kit; IL18 binding protein ELISA Kit; IL18 BP ELISA Kit; IL18 BPa ELISA Kit; IL18BP ELISA Kit; IL18BPa ELISA Kit; Interleukin 18 binding protein ELISA Kit; Interleukin-18-binding protein ELISA Kit; MC51L 53L 54L homolog gene product ELISA Kit; Tadekinig alfa ELISA Kit; Tadekinig-alfa ELISA Kit
-
缩写：
-
Uniprot No.：
-
种属：Homo sapiens (Human)
-
样本类型：serum, plasma
-
检测范围：15.6 pg/ml-1000 pg/ml
-
灵敏度：3.9 pg/ml
-
反应时间：1-5h
-
样本体积：50-100ul
-
检测波长：450 nm
-
研究领域：Immunology
-
测定原理：quantitative
-
测定方法：Sandwich
-
精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean(pg/ml)
128.430
124.396
125.740
124.844
128.205
126.861
SD
0.032
0.035
0.037
0.050
0.046
0.047
CV(%)
4.819
5.418
5.675
7.716
6.938
7.154
-
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human IL-18BP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:10
Average %
100
Range %
95-104
1:20
Average %
84
Range %
80-88
1:40
Average %
98
Range %
92-103
1:80
Average %
90
Range %
85-97
-
回收率：
The recovery of human IL-18BP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
95
90-100
EDTA plasma (n=4)
93
89-98
-
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
1000
2.412
2.624
2.518
2.412
500
1.845
1.952
1.899
1.793
250
1.181
1.124
1.153
1.047
125
0.685
0.645
0.665
0.559
62.5
0.371
0.393
0.382
0.276
31.2
0.234
0.223
0.229
0.123
15.6
0.169
0.162
0.166
0.060
0
0.107
0.105
0.106
-
数据处理：
-
货期：3-5 working days
引用文献
产品评价
平均分:
样品类型：血浆（抗凝剂） EDTA
样品信息：人
稀释比：其他 1:20
产品评价： 我用CSB-E13687h检测系统性红斑狼疮病人血浆样本中的IL-18BP, 标准品OD范围是0.06-1.75. 血浆样本OD值范围是0.8-1.5，标准曲线R平方约0.99, 依据标准曲线算出的样本浓度范围是8-17ng/mL, 符合预期结果。
By 唐老师
相关产品
靶点详情
-
功能：Isoform A binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response.
-
基因功能参考文献：
- these results were corroborated in female osteoporotic subjects where decreased serum IL-18BP levels and enhanced serum IL-18 levels were observed. Our study forms a strong basis for using humanized IL-18BP towards the treatment of postmenopausal osteoporosis. PMID: 27649785
- epigenetic silencing by single CpG methylation determines differential IL18BP inducibility in monocytic versus epithelial cells. T PMID: 29409936
- a large proportion blood basophils from patients with asthma express IL-18 and IL-18BP. mast cells and basophils are implicated in the pathogenesis of asthma via an IL-18-related mechanism. PMID: 29505668
- The maintenance of normal production of IL-18BP may contribute, at least in part, to the ability ofLong Term Non-Progressors to delay AIDS progression. PMID: 27863336
- Platelets also contain the IL-18 antagonist, the IL-18-Binding Protein (IL-18BP); however, it is not synthesized in them de novo, is present in pre-made form and is released irrespective of platelet activation. PMID: 27914933
- The serum levels of IL-37, which were correlated with antibody production and the serum levels of total IL-18 and IL-18BP, were elevated in the patients with primary Sjogren's syndrome. PMID: 28057714
- an imbalance in the production of IL-18 and its antagonist (an increase in the production of IL-18 with a decrease, no increase or an insufficient increase in the production of IL-18BP) has been described in many chronic inflammatory diseases in humans. PMID: 26898120
- IL-18, IL-18R and IL-18BP expression in eosinophil are involved in the inflammatory reaction of asthma. PMID: 28395725
- the inhibition of IL-18 signaling by IL-18BPa may be involved in the development of pulmonary vascular involvement leading to pulmonary hypertension and modulate the systemic inflammation in systemic sclerosis PMID: 26777734
- The natural IL-18 inhibitor IL-18BP further regulates IL-18 activity in the extracellular environment. Review. PMID: 25548255
- imbalance of IL-18/IL-18BP ratio in IgA nephropathy especially in patients with arteriolar lesions PMID: 25807634
- IL-18, IL-18BP, and IL-18R may have roles in the pathogenesis of epithelial ovarian carcinoma PMID: 24963217
- HPV16 E7 oncoprotein increases production of the IL18BP in keratinocytes. PMID: 24478434
- these data indicate that IL-18BP, which is produced in EOC in response to microenvironmental factors, may inhibit endogenous or exogenous IL-18 activity PMID: 23873689
- Data suggest that the imbalance of IL-18/IL-18BP might play an important role in the pathogenesis of systemic juvenile idiopathic arthritis (SJIA) and it might go hand-in-hand with the severity of SJIA. PMID: 23419721
- Due to highly significant increases in circulating IL-18BP in schizophrenia compared to controls, the levels of free IL-18 are not significantly different between schizophrenic groups. PMID: 22913567
- Imbalance between IL-18 and IL-18BP may play an important role in pathogenesis of idiopathic thrombocytopenic purpura. PMID: 21867627
- High IL-18 BP levels indicate the severity of existing glomerular injury in systemic lupus erythematosus. PMID: 21656327
- The balance between interleukin-18 (IL-18) and its endogenous antagonist, IL-18 binding protein (IL-18BP), was evaluated in children with Henoch-Schonlein purpura. PMID: 22289535
- IL-18/IL-18BP imbalance plays an important role in pathogenesis of primary immune thrombocytopenia. PMID: 21418867
- In T2DM patients, 18 BP began to increase after IL-18 increased and reached a threshold, in which case kidney dysfunction would have developed. PMID: 21440322
- increased levels of both IL-18 and its natural inhibitor IL-18BP, characterise SLE PMID: 21126942
- In lines of intestinal epithelial cells from inflammatory bowel disease patients, interferon-gamma selectively up-regulated IL-18 binding protein. PMID: 21078084
- lupus nephritis patients present lower IL-18BP mRNA expression and higher serum levels of IL-18 than those in normal controls PMID: 20140691
- Altogether, data presented herein indicate that direct action of STAT1 on the IL-18BP promoter at the proximal GAS element is key to IL-18BP expression by IFN-gamma-stimulated DLD-1 colon carcinoma cells PMID: 19046253
- High circulating levels in patients with active Systemic Lupus Erythematosus PMID: 19699611
- Endometrial interleukin-18 binding protein mRNA expression may possibly be responsible for the pathologic process of adenomyosis. PMID: 19394601
- Suggest that an early rise in IL-18 levels may play a role in reverse remodeling process following aortic valve replacement. PMID: 19961286
- Since IL-18 plays a major role in perpetuating hemophagocytosis, the failure of IFNgamma to induce IL-18BP may constitute a fundamental pathogenetic mechanism PMID: 20072626
- trancriptional activation and release of IL-18 binding protein in response to IFN-gamma PMID: 11739524
- IL-18BP messenger transcript and protein are significantly increased in surgically resected specimens from active Crohn's disease compared with control patients, correlating with an up-regulation of IL-18. PMID: 11907126
- after binding to IL-18BP, IL-1F7b forms a complex with IL-18Rbeta, depriving the beta-chain of forming a functional receptor complex with IL-18Ralpha and thus inhibiting IL-18 activity PMID: 12381835
- IRF-1-CEBPbeta complex activate the promoter of IL-18 binding protein. PMID: 12482935
- Administration of recombinant human IL-18BP not only reduces symptoms of murine contact hypersensitivity (CHS) during the elicitation phase but also significantly decreases inflammation in mice that had previously undergone CHS without treatment. PMID: 12874202
- High endogenous levels of IL-18BP in trangenic mice effectively neutralize IL-18 and are protective in response to different inflammatory stimuli. PMID: 12960225
- production of IL-18BP in response to IL-12 and IL-18 was regulated differently in blood and synovial cells. PMID: 15188356
- IL-18 levels, which are determined in part by variation in IL18/IL18BP, play a role in coronary heart disease development and postsurgery outcome. PMID: 17951325
- Despite the elevated IL-18BP levels during active disease, free IL-18 remained higher than in the inactive disease stages, suggesting a potential benefit of administration of exogenous IL-18BP as a therapeutic approach for active Wegener's granulomatosis. PMID: 18594952
收起更多
-
亚细胞定位：Secreted.
-
组织特异性：Strongly expressed in heart, lung, placenta and spleen.
-
数据库链接：
