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Human irisin ELISA kit

  • 中文名称:
    人鸢尾素酶联免疫试剂盒
  • 货号:
    CSB-EQ027943HU
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

产品详情

  • 产品描述:
    人鸢尾素酶联免疫试剂盒(CSB-EQ027943HU)为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的irisin含量。Irisin是一种由运动诱导产生的肌肉因子。背景上,它被视为运动益处的重要介质。研究机制方面,Irisin能促进白色脂肪棕色化,增加能量消耗,还参与调节葡萄糖代谢、骨代谢等过程,在代谢性疾病等领域有潜在治疗价值。试剂盒检测范围为3.12 ng/mL-200 ng/mL,科研人员可将其应用于运动生理学、代谢调控机制、脂肪组织功能研究等领域,通过定量分析不同干预条件下(如运动训练、药物处理)样本中鸢尾素浓度变化,为探索代谢相关疾病的分子机制提供可靠数据支持。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 缩写:
    irisin
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    3.12 ng/mL-200 ng/mL
  • 灵敏度:
    0.78 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Others
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human irisin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
      Sample Serum(n=4)    
    1:1 Average % 90    
    Range % 84-96    
    1:2 Average % 97    
    Range % 91-103    
    1:4 Average % 103    
    Range % 97-109    
    1:8 Average % 89    
    Range % 83-95    
  • 回收率:
    The recovery of human irisin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 100 94-106    
    EDTA plasma (n=4) 106 101-112    
                   
                   
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average Corrected    
    200 2.690 2.752 2.721 2.616    
    100 2.402 2.371 2.387 2.282    
    50 1.965 1.853 1.909 1.804    
    25 1.355 1.247 1.301 1.196    
    12.5 0.764 0.735 0.750 0.645    
    6.25 0.495 0.464 0.480 0.375    
    3.12 0.251 0.266 0.259 0.154    
    0 0.106 0.103 0.105      
  • 数据处理:
  • 货期:
    3-5 working days

引用文献

产品评价

靶点详情

  • 最新研究进展:
    Irisin是一种由肌肉细胞产生的激素,在身体代谢调节中发挥着重要作用。最近的研究表明,Irisin对改善糖尿病、肥胖和心血管疾病等慢性病的预防和治疗具有潜在价值。此外,研究人员正在探索Irisin对其他疾病的影响,如阿尔茨海默病、肌肉萎缩和骨质疏松症等。虽然Irisin的作用机制还没有完全理解,但这种激素已经成为新的药物开发领域的热门研究方向之一。