Human leucocyte antigen B27,HLA-B27 ELISA Kit

产品详情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO人类白细胞抗原B27（HLA-B27）酶联免疫试剂盒（货号：CSB-E08607h），采用双抗体夹心法原理实现定量检测，适用于血清、尿液及脑脊液样本中HLA-B27蛋白的科研分析。HLA-B27作为重要的I型人类白细胞抗原，与强直性脊柱炎、反应性关节炎等疾病的免疫调控机制研究密切相关。试剂盒检测灵敏度达0.312 ng/mL，线性范围为0.312-20 ng/mL，可精准覆盖生物样本中HLA-B27的基础浓度至较高表达水平。通过预包被特异性捕获抗体和标记检测抗体的协同作用，实现对目标蛋白的特异性识别与定量分析。该试剂盒可为免疫性疾病机制研究、炎症反应通路探索以及HLA-B27表达水平与疾病相关性分析提供可靠工具，尤其适用于神经免疫、风湿病学等领域的体液样本检测需求，其多样本类型的兼容性为跨组织液比较研究创造了便利条件。
缩写：

HLA-B27
Uniprot No.：

P03989
种属：

Homo sapiens (Human)
样本类型：

serum, urine, cerebrospinal fluid (CSF)
检测范围：

0.312 ng/mL-20 ng/mL
灵敏度：

0.078 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human HLA-B27 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	80-98
1:2	Average %	86
	Range %	80-96
1:4	Average %	96
	Range %	92-100
1:8	Average %	100
	Range %	96-110

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.