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  4. Human plasma kallikrein(KLKB1) ELISA kit

Human plasma kallikrein(KLKB1) ELISA kit

文献引用 (1) 说明书下载
  • 中文名称：
    人血浆激肽释放酶(KLKB1)酶联免疫试剂盒
  • 货号：
    CSB-E14339h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO人血浆激肽释放酶（KLKB1）定量检测试剂盒（货号：CSB-E14339h），基于双抗体夹心法原理设计，可精准检测血清或血浆样本中KLKB1的酶活性水平。KLKB1属于丝氨酸蛋白酶家族，在激肽-激肽释放酶系统中发挥核心作用，参与调节血管舒张、炎症反应及凝血级联等生理过程，其表达异常与血栓形成、遗传性血管性水肿等病理机制密切相关。CUSABIO试剂盒采用高特异性配对抗体，检测灵敏度达9.38 mU/mL，线性范围覆盖9.38-600 mU/mL，适用于不同浓度样本的精准分析，操作流程包含标准品预包被、样本孵育、酶标抗体结合及显色反应等步骤，实验全程约需4小时。该产品特别适用于基础科研领域，可用于研究KLKB1在心血管疾病、炎症反应、纤维化疾病中的功能调控机制，或用于评估药物干预对激肽释放酶系统的影响，为探索相关疾病的分子机制及潜在治疗靶点提供可靠工具，实验需配套酶标仪在450nm波长下进行吸光度测定。
  • 别名：
    Fletcher factor ELISA Kit; kallikrein B plasma ELISA Kit; Kallikrein B plasma (Fletcher factor) 1 ELISA Kit; Kallikrein B; plasma 1 ELISA Kit; Kallikrein B1 ELISA Kit; Kininogenin ELISA Kit; KLK3 ELISA Kit; KLKB1 ELISA Kit; KLKB1_HUMAN ELISA Kit; PKK ELISA Kit; PKKD ELISA Kit; Plasma kallikrein ELISA Kit; Plasma kallikrein B1 ELISA Kit; Plasma kallikrein light chain ELISA Kit; Plasma prekallikrein ELISA Kit; PPK ELISA Kit; Prekallikrein ELISA Kit
  • 缩写：
    KLKB1
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma
  • 检测范围：
    9.38 mU/mL-600 mU/mL
  • 灵敏度：
    2.34 mU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human KLKB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %96
    Range %89-100
    1:200Average %90
    Range %85-98
    1:400Average %87
    Range %83-93
    1:800Average %92
    Range %88-96
  • 回收率：
    The recovery of human KLKB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9480-103
    EDTA plasma (n=4)9686-106
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/mlOD1OD2AverageCorrected
    6002.101 2.207 2.154 2.048
    3001.695 1.687 1.691 1.585
    1501.261 1.278 1.270 1.164
    750.842 0.867 0.855 0.749
    37.50.503 0.514 0.509 0.403
    18.750.345 0.368 0.357 0.251
    9.380.201 0.211 0.206 0.100
    00.105 0.107 0.106 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

产品评价

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KLKB1 antibodies

KLKB1 Proteins

相关问答

■ 常见问题解答
Q:

Does the ELISA detect both the zymogen and the cleaved Klkb1 or only the zymogen?

A:
Thanks for your inquiry.
CSB-E14339h
This kit is designed against full length.
Pls refer to: https://www.uniprot.org/uniprot/P03952.Pls let me know if you have any further questions. Thank you.

靶点详情

  • 功能：
    The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
  • 基因功能参考文献：
    1. We further define the interactions of keratinocyte PKK with TP63 and NF-kappaB signaling, highlighting the importance of this protein as a tumor suppressor in skin squamous cell carcinoma development. PMID: 29186361
    2. Plasma kallikrein (PLK)-dependent transforming growth factor-beta (TGF-beta) activation could be detected in isolated pancreatic stellate cells (PSCs) from chronic pancreatitis (CP) and pancreatic cancer. PMID: 28187111
    3. High molecular weight kinin functions as a cofactor for PK activation in the presence of nucleic acids in a manner consistent with classic models of contact activation. PMID: 28124063
    4. genetic polymorphism is associated with C1 Inhibitor deficiency angioedema age of onset in European families PMID: 29130992
    5. these data indicate that KLKB1 induces inflammatory reactions in human dental tissues via protease-activated receptor 1 PMID: 26566265
    6. von Willebrand factor activity and concentration of prekallikrein may both be of importance regarding the evolution of thrombus in abdominal aortic aneurysm and possible biomarkers for aneurysm growth. PMID: 27581227
    7. KLKB1 mRNA expression is a putative molecular biomarker in chronic lymphocytic leukemia. PMID: 25891023
    8. Genotyping these subjects revealed that the carriers of the minor alleles at the two loci- F12 and KLKB1 had a significant association with reduced levels of active plasma renin. PMID: 26969407
    9. 2 genetic loci (kininogen 1 and kallikrein B) influencing key components of the RAAS, consistent with the close interrelation between the kallikrein-kinin system and the RAAS. PMID: 25477429
    10. PRCP1 interacts with plasma kallikrein (PK) at multiple sites for PK activation. PMID: 25324000
    11. Prekallikrein deficiency in a family is due to a new mutation (Arg541Gln) in exon 14. PMID: 25075649
    12. FXI may have a role in risk of ischemic stroke, but not myocardial infarct; FXII and prekallikrein may not have a role in either PMID: 24977287
    13. The acquisition of an active site in prekallikrein as a result of binding to high-molecular-weight kininogen (HK) is a new concept for bradykinin formation and might be relevant in the pathogenesis of hereditary angiodema types I and II. PMID: 23672780
    14. Investigate the relationship between circulating PK levels in children with metabolic syndrome and CVD risk factors because PK may be involved in the progression of the disease state. PMID: 20725119
    15. Case Report: Fresh frozen plasma transfusion before coronary artery bypass corrects prekallikrein deficiency. PMID: 20135073
    16. plasma kallikrein and FXIa activate pro-HGF in vitro PMID: 12372819
    17. the effect of kininogen degradation by human neutrophil elastase (HNE) on kinin generation by tissue and plasma kallikreins. PMID: 12887060
    18. prekallikrein preferential binding to endothelial cells contributes to their anticoagulant nature PMID: 12944405
    19. Crystallization and x-ray crystal structure determination have yielded the first three-dimensional views of the catalytic domain of plasma kallikrein PMID: 16199530
    20. Our findings suggested that common genetic variation in the KLKB1 gene might contribute to the risk of hypertension in the northern Han Chinese population. PMID: 17318641
    21. study demonstrates by immunohistochemistry that plasma kallikrein (PK) is localised in cells of different embryologically derived human tissues PMID: 17696780
    22. three-dimensional structural data for prekallikrein (PK); interaction between PK and high-molecular-weight kininogen is mediated by two discrete surfaces formed by the PK A1, A2 and A4 domains with charge likely to be a critical component of the binding PMID: 17922805
    23. Kallikrein can directly activate prothrombin; there is a shortcut in the intrinsic hemostasis system that generates catalytic amounts of thrombin without following the known intrinsic clotting pathway PMID: 18160576
    24. Kallikrein is responsible for the cleavage of factor H. PMID: 18413232
    25. prekallikrein is an enzyme that can cleave high-molecular-weight kininogen to release bradykinin, and this reaction is inhibited by C1 inhibitor. PMID: 19342086
    26. NPY3-35 is a new peptide generated by plasma kallikrein PMID: 19620246

    收起更多

  • 相关疾病：
    Prekallikrein deficiency (PKK deficiency)
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Peptidase S1 family, Plasma kallikrein subfamily
  • 数据库链接：

    HGNC: 6371

    OMIM: 229000

    KEGG: hsa:3818

    STRING: 9606.ENSP00000264690

    UniGene: Hs.237642

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