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  4. Human tryptophanyl-tRNA synthetase,WARS ELISA Kit

Human tryptophanyl-tRNA synthetase,WARS ELISA Kit

文献引用 (3) 说明书下载
  • 中文名称：
    人色氨酰tRNA合成酶(WARS)酶联免疫试剂盒
  • 货号：
    CSB-E11789h
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO人色氨酰tRNA合成酶（WARS）酶联免疫检测试剂盒（货号CSB-E11789h），基于双抗体夹心法原理实现定量检测，适用于血清、血浆、细胞裂解液及组织匀浆等样本中WARS蛋白的精确分析。WARS作为氨酰tRNA合成酶家族成员，负责催化色氨酸与对应tRNA的结合，是蛋白质生物合成的关键起始酶，近年研究显示其在细胞应激反应和疾病发生中可能具有调控作用。该试剂盒检测灵敏度达0.312 ng/mL，线性检测范围为0.312-20 ng/mL，可在3.5小时内完成96孔板规格的检测流程，标准品与预包被板均采用即用型设计，配套提供检测所需缓冲液与终止液，试剂盒保存于2-8℃环境下有效期达6个月。适用于基础科研领域中对细胞应激机制、信号通路调控或疾病模型（如炎症、肿瘤等）中WARS蛋白表达水平变化的定量研究，为探索该酶在细胞生物学过程中的功能提供可靠工具。本产品严格限定于科学研究用途，实验操作需遵循标准生物安全规范。
  • 别名：
    Gamma 2 ELISA Kit; GAMMA-2 ELISA Kit; Gamma2 ELISA Kit; hWRS ELISA Kit; IFI 53 ELISA Kit; IFI53 ELISA Kit; IFP 53 ELISA Kit; IFP53 ELISA Kit; Interferon induced protein 53 ELISA Kit; Interferon-induced protein 53 ELISA Kit; SYWC_HUMAN ELISA Kit; T2-TrpRS ELISA Kit; TrpRS ELISA Kit; Tryptophan tRNA ligase; cytoplasmic ELISA Kit; Tryptophan tRNA ligase 1 cytoplasmic ELISA Kit; Tryptophan tRNA ligase ELISA Kit; Tryptophan--tRNA ligase ELISA Kit; Tryptophanyl tRNA synthetase ELISA Kit; Tryptophanyl tRNA synthetase cytoplasmic ELISA Kit; WARS ELISA Kit; WARS protein ELISA Kit; WRS ELISA Kit
  • 缩写：
    WARS
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell lysates, tissue homogenates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Epigenetics and Nuclear Signaling
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human WARS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 91  
    Range % 88-95  
    1:2 Average % 87  
    Range % 81-93  
    1:4 Average % 97  
    Range % 93-101  
    1:8 Average % 100  
    Range % 94-106  
  • 回收率：
    The recovery of human WARS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 89 84-95  
    EDTA plasma (n=4) 93 88-99  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    20 1.972 2.050 2.011 1.920  
    10 1.446 1.556 1.501 1.410  
    5 1.027 1.101 1.064 0.973  
    2.5 0.638 0.606 0.622 0.531  
    1.25 0.381 0.387 0.384 0.293  
    0.625 0.293 0.275 0.284 0.193  
    0.312 0.195 0.201 0.198 0.107  
    0 0.093 0.089 0.091 　  
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

产品评价

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WARS antibodies

WARS Proteins

靶点详情

  • 功能：
    Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression.
  • 基因功能参考文献：
    1. Based on these results, secretion of full-length tryptophanyl-tRNA synthetase appears to work as a primary defence system against infection, acting before full activation of innate immunity. PMID: 27748732
    2. findings establish WARS as a gene whose mutations may cause distal hereditary motor neuropathy and alter canonical and non-canonical functions of tryptophanyl-tRNA synthetase. PMID: 28369220
    3. Overexpression of WARS predicts no recurrence and good survival for triple-negative breast cancer patients. PMID: 26209610
    4. Tryptophanyl-tRNA synthetase expression is up-regulated in patients with rheumatoid arthritis. PMID: 24515434
    5. Genes within recently identified loci associated with waist-hip ratio (WHR) exhibit fat depot-specific mRNA expression, which correlates with obesity-related traits. Adipose tissue (AT) mRNA expression of 6 genes (TBX15/WARS2, STAB1, PIGC, ZNRF3, GRB14) PMID: 23670221
    6. Indoleamine2,3-dioxygenase and tryptophanyl-tRNA synthetase may play critical roles in the immune pathogenesis of chronic kidney disease. PMID: 23651343
    7. Naturally occurring fragments of the two proteins involved in translation, TyrRS and TrpRS, have opposing activities on angiogenesis. PMID: 21442253
    8. Tryptophanyl-tRNA synthetase down-regulation by hypoxia may be a factor responsible for low TrpRS in pancreatic tumors with high metastatic ability. PMID: 21926542
    9. Mini-tryptophanyl-tRNA synthetase inhibited ischemic angiogenesis in rats. PMID: 20963594
    10. Tryptophanyl-tRNA synthetase is a multidomain protein exhibiting excellent allosteric communication, and this research has provided valuable structural as well as functional insights into the protein. PMID: 19768679
    11. Low tryptophanyl-tRNA synthetase is associated with recurrence in colorectal cancer. PMID: 19900940
    12. In this study, we show that a recombinant form of a COOH-terminal fragment of TrpRS is a potent antagonist of vascular endothelial growth factor-induced angiogenesis in a mouse model and of naturally occurring retinal angiogenesis in the neonatal mouse PMID: 11773625
    13. Thus, protein synthesis may be linked to the regulation of angiogenesis by a natural fragment of TrpRS PMID: 11773626
    14. Recognition by tryptophanyl-tRNA synthetases of discriminator base on tRNATrp from three biological domains PMID: 11834741
    15. The recently discovered antiangiogenic and cell-signaling activities of tryptophanyl-tRNA synthetase bioactive fragments are discussed in this review. PMID: 12416978
    16. TrpRS may have a role in the maintenance of vascular homeostasis PMID: 14630953
    17. results suggest that mammalian and bacterial tryptophanyl-tRNA synthetase might use different mechanisms to recognize the substrate and modeling studies indicate that transfer RNA binds with the dimeric enzyme PMID: 14660560
    18. A crystal structure of human tryptophanyl-tRNA synthetase was solved at 2.1 A with a tryptophanyl-adenylate bound at the active site PMID: 14671330
    19. May play an important role in the intracellular regulation of protein synthesis under conditions of oxidative stress. PMID: 15628863
    20. These crystals captured two conformations of the human tryptophanyl-tRNA synthetase and tRNATrp complex, which are nearly identical with respect to the protein and a bound tryptophan. PMID: 16724112
    21. The first crystal structure of human tryptophanyl-tRNA synthetase (hTrpRS) in complex with tRNA(Trp) and Trp which, together with biochemical data, reveals the molecular basis of a novel tRNA binding and recognition mechanism. PMID: 16798914
    22. Results provide the first evidence of the involvement of heme in regulation of TrpRS aminoacylation activity. PMID: 17877375
    23. the annexin II-S100A10 complex, which regulates exocytosis, forms a ternary complex with TrpRS. PMID: 17999956
    24. Analysis of the molecular basis of the mechanisms of the substrate recognition and the activation reaction by tryptophanyl-tRNA synthetase. PMID: 18180246
    25. Indoleamine 2,3-dioxygenase (IDO)-expression in antigen-presenting cells (APCs) may control autoimmune responses by depleting the available tryptophan, whereas tryptophanyl-tRNA synthetase (TTS) may counteract this effect PMID: 19363598

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Class-I aminoacyl-tRNA synthetase family
  • 数据库链接：

    HGNC: 12729

    OMIM: 191050

    KEGG: hsa:7453

    STRING: 9606.ENSP00000347495

    UniGene: Hs.497599

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