Human tumor necrosis factor-related apoptosis-inducing ligand receptor 3,TRAIL-R3 ELISA Kit
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中文名称：人肿瘤坏死因子相关凋亡诱导配体受体3(TRAIL-R3)酶联免疫试剂盒
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货号：CSB-E04748h
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规格：96T/48T
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价格：￥3200/￥2500
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其他：
产品详情
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产品描述：CUSABIO人肿瘤坏死因子相关凋亡诱导配体受体3（TRAIL-R3）酶联免疫试剂盒（货号：CSB-E04748h），采用双抗体夹心法定量检测血清、血浆及组织匀浆样本中的TRAIL-R3蛋白浓度，检测范围为78 pg/mL至5000 pg/mL。TRAIL-R3是肿瘤坏死因子受体超家族成员之一，通过与TRAIL配体结合参与调控细胞凋亡过程，其异常表达可能与肿瘤免疫逃逸等病理过程相关。该试剂盒适用于基础科研领域，例如探究肿瘤发生机制、细胞凋亡信号通路调控等研究场景，可为体外分析不同生物样本中TRAIL-R3的蛋白表达水平提供可靠工具。产品操作流程基于标准ELISA技术，包含预包被特异性抗体的酶标板及配套试剂，支持科研人员在肿瘤生物学、免疫学等领域开展实验研究。
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别名：TNFRSF10C ELISA Kit; DCR1 ELISA Kit; LIT ELISA Kit; TRAILR3 ELISA Kit; TRID ELISA Kit; UNQ321/PRO366 ELISA Kit; Tumor necrosis factor receptor superfamily member 10C ELISA Kit; Antagonist decoy receptor for TRAIL/Apo-2L ELISA Kit; Decoy TRAIL receptor without death domain ELISA Kit; Decoy receptor 1 ELISA Kit; DcR1 ELISA Kit; Lymphocyte inhibitor of TRAIL ELISA Kit; TNF-related apoptosis-inducing ligand receptor 3 ELISA Kit; TRAIL receptor 3 ELISA Kit; TRAIL-R3 ELISA Kit; TRAIL receptor without an intracellular domain ELISA Kit; CD antigen CD263 ELISA Kit
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缩写：TRAIL-R3
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Uniprot No.：
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种属：Homo sapiens (Human)
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样本类型：serum, plasma, tissue homogenates
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检测范围：78 pg/mL-5000 pg/mL
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灵敏度：19.5 pg/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Cancer
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测定原理：quantitative
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测定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean(pg/ml)
625.668
628.643
626.411
627.471
624.154
623.666
SD
0.059
0.048
0.052
0.061
0.055
0.066
CV(%)
5.004
4.057
4.407
5.163
4.673
5.611
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线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human TRAIL R3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
101
Range %
88-105
1:2
Average %
104
Range %
95-109
1:4
Average %
94
Range %
87-98
1:8
Average %
104
Range %
99-108
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回收率：
The recovery of human TRAIL R3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range%
Serum (n=5)
106
100-109
EDTA plasma (n=4)
101
95-105
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标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml
OD1
OD2
Average
Corrected
5000
2.652
2.504
2.578
2.408
2500
2.326
2.242
2.284
2.114
1250
1.736
1.765
1.751
1.581
625
1.170
1.189
1.180
1.010
312
0.716
0.698
0.707
0.537
156
0.515
0.500
0.508
0.338
78
0.280
0.281
0.281
0.111
0
0.168
0.172
0.170
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数据处理：
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货期：3-5 working days
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靶点详情
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功能：Receptor for the cytotoxic ligand TRAIL. Lacks a cytoplasmic death domain and hence is not capable of inducing apoptosis. May protect cells against TRAIL mediated apoptosis by competing with TRAIL-R1 and R2 for binding to the ligand.
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基因功能参考文献：
- High levels of TRAIL-R3 and CCR-2 expression in tumor epithelial cells identified patients with early breast cancer with poor outcomes. PMID: 28420351
- DcR1 levels in serum sample which were significantly lower in AMD patients. PMID: 24534820
- DcR1 upregulation mediates temozolomide resistance. PMID: 25808868
- GATA4 and DcR1 promoter hypermethylation is tumor specific event in glioblastoma but they promoter methylation cannot be considered as a prognostic marker of glioblastoma survival. PMID: 23320456
- Primary EOC is associated to a lower TRAIL-R3 expression. PMID: 22555108
- Data show that about 20% of AML patients highly expressed decoy receptor TRAIL-R3, which was strongly correlated to a shortened overall survival. PMID: 21281967
- Decoy receptors DcR1 and DcR2 on CD8+ T cells, but not on CD4-positive T cells, are positively correlated with patients' DAS scores. PMID: 20799941
- Results suggest that DcR1 expression occurs in a subset of endometrial carcinomas and may contribute to resistance to TRAIL-induced apoptosis. PMID: 19936781
- analysis of the transcription initiation sites and promoter structure of the TRAIL-R3 gene PMID: 12417331
- cloned and characterized a p53 consensus element located within the first intron of the TRAIL-R3 gene PMID: 14623878
- Our results demonstrate that DcR1 and DcR2 genes are frequently methylated in various tumor types, and aberrant methylation was the cause for silencing of DcR1 and DcR2 expression. PMID: 14999791
- The DcR1 had no death domain and was anchored to the membrane via a glycophosphatidyl inositol tail. PMID: 15538968
- Resistance to TRAIL-induced apoptosis in acute myeloid leukemia cells is associated with expression of TRAIL-R3. PMID: 15921376
- tryptophol induces apoptosis through DR5 and the resistance of PBL to tryptophol-induced apoptosis might be due to competition from DcR1 PMID: 17690453
- p53 negatively regulates oxaliplatin-mediated TRAIL-induced apoptotic activity through DcR1 upregulation. PMID: 18345033
- TRAILR3 (TNF-related apoptosis inducing ligand receptor 3) levels were significantly higher in lung parenchyma in subjects with emphysema PMID: 18511705
- Liver metastases with low DcR1/TNFRSF10C mRNA expression were more likely to present with extrahepatic metastases PMID: 18590575
- inactivation of TNFRSF10C by chromosomal deletion and promoter methylation may play an important role in prostate cancer development PMID: 19035483
- Low DCR1 is associated with non-small cell lung cancer. PMID: 19661294
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亚细胞定位：Cell membrane; Lipid-anchor, GPI-anchor.
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组织特异性：Higher expression in normal tissues than in tumor cell lines. Highly expressed in peripheral blood lymphocytes, spleen, skeletal muscle, placenta, lung and heart.
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数据库链接：
