Monkey Complement 3(C3)ELISA Kit
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中文名称：
猴子补体蛋白3(C3)酶联免疫试剂盒
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货号：
CSB-E16528Mk
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规格：
96T/48T
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价格：
￥4200/￥3000
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其他：
产品详情
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产品描述：
CUSABIO针对猴源补体蛋白3（C3）的定量检测酶联免疫试剂盒（货号：CSB-E16528Mk），采用双抗体夹心法原理，可精准检测血清、血浆及细胞培养上清液样本中的C3蛋白水平，检测范围为4.68 ng/mL至300 ng/mL。补体蛋白C3是补体系统的核心成分之一，在先天免疫应答中发挥关键作用，参与调理吞噬、炎症反应及膜攻击复合物形成等过程，其水平变化常与感染、炎症或免疫相关疾病的病理机制研究密切相关。本试剂盒提供标准化的预包被板、检测抗体及操作试剂，适用于科研领域中对猴模型（如食蟹猴、恒河猴等）的免疫学研究，例如评估疾病模型中补体系统的激活状态、药物或疫苗干预后免疫应答的动态变化，或体外实验中细胞因子诱导的补体通路调控分析。实验操作简便，需使用酶标仪在450 nm波长下读取吸光度值，结合标准曲线实现目标蛋白的定量分析。
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别名：
C3Complement C3 [Cleaved into: Complement C3 beta chain; Complement C3 alpha chain; C3a anaphylatoxin; C3-beta-c ELISA kit; C3bc); Acylation stimulating protein ELISA kit; ASP ELISA kit; C3adesArg); Complement C3b alpha' chain; Complement C3c alpha' chain fragment 1; Complement C3dg fragment; Complement C3g fragment; Complement C3d fragment; Complement C3f fragment; Complement C3c alpha' chain fragment 2] ELISA kit
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缩写：
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Uniprot No.：
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种属：
Monkey
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样本类型：
serum, plasma, cell culture supernates
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检测范围：
4.68 ng/mL-300 ng/mL
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灵敏度：
1.17 ng/mL
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反应时间：
1-5h
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样本体积：
50-100ul
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检测波长：
450 nm
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研究领域：
Cancer
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测定原理：
quantitative
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测定方法：
Sandwich
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精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 300 |
2.276 |
2.296 |
2.286 |
2.144 |
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| 150 |
1.896 |
1.854 |
1.875 |
1.733 |
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| 75 |
1.520 |
1.539 |
1.530 |
1.388 |
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| 37.5 |
1.195 |
1.174 |
1.185 |
1.043 |
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| 18.75 |
0.864 |
0.883 |
0.874 |
0.732 |
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| 9.37 |
0.531 |
0.557 |
0.544 |
0.402 |
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| 4.68 |
0.345 |
0.365 |
0.355 |
0.213 |
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| 0 |
0.141 |
0.143 |
0.142 |
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数据处理：
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货期：
3-5 working days
靶点详情
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功能：
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.; Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. In chronic inflammation, acts as a chemoattractant for neutrophils.; Acts as a chemoattractant for neutrophils in chronic inflammation.; adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-internalization and recycling of C5AR2.
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基因功能参考文献：
- The structure of bovine C3 clearly demonstrates that the main chain around the thioester undergoes a helical transition upon activation, a rearrangement that is proposed to be the basis for the high level of reactivity of the thioester group. PMID: 16831446
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亚细胞定位：
Secreted.
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数据库链接：
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