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Monkey Interferon γ,IFN-γ ELISA Kit

  • 中文名称:
    猴γ干扰素(IFN-γ)酶联免疫试剂盒
  • 货号:
    CSB-E10049Mo
  • 规格:
    96T/48T
  • 价格:
    ¥4200/¥3000
  • 其他:

产品详情

  • 产品描述:
    猴γ干扰素(IFN-γ)酶联免疫试剂盒(CSB-E10049Mo)为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的IFNG含量。IFNG即干扰素-γ,在免疫调节等方面背景重要。它是由活化的T细胞和NK细胞产生的细胞因子。研究机制上,可激活巨噬细胞、增强MHC分子表达、调节Th1/Th2平衡等,在机体免疫防御、抗肿瘤等过程中发挥关键作用。试剂盒检测范围为31.25 pg/mL-2000 pg/mL,支持科研人员开展非人灵长类动物模型的免疫机制研究、感染性疾病模型评估或药物干预实验中的细胞因子动态监测本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    IFNG ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit
  • 缩写:
  • Uniprot No.:
  • 种属:
    Monkey
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    31.25 pg/mL-2000 pg/mL
  • 灵敏度:
    7.8 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20001.830 1.990 1.910 1.805
    10001.496 1.398 1.447 1.342
    5001.133 1.138 1.136 1.031
    2500.932 0.902 0.917 0.812
    1250.544 0.535 0.540 0.435
    62.50.212 0.226 0.219 0.114
    31.250.117 0.111 0.114 0.009
    00.106 0.104 0.105  
  • 数据处理:
  • 货期:
    3-5 working days

产品评价

靶点详情

  • 功能:
    Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL. Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation.
  • 基因功能参考文献:
    1. IFNgamma expression in colonic epithelial cells was regulated by TGFB1. PMID: 25769244
    2. Higher KIR2DL4 copy numbers is associated with an increased IFN-gamma production in NK cell subsets in SIV-infected Mamu-A*01-negative rhesus macaques. PMID: 23449795
    3. Data show that viral set-point in simian immunodeficiency virus diseasewas is associated with expression of interferon gamma -stimulated genes. PMID: 17719201
    4. Low Sociable animals showed alterations in lymph node expression of the immunoregulatory cytokine interferon-gamma. PMID: 18068331
  • 亚细胞定位:
    Secreted.
  • 蛋白家族:
    Type II (or gamma) interferon family
  • 组织特异性:
    Released primarily from activated T lymphocytes.
  • 数据库链接: