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中文名称:猴β淀粉样蛋白1-42(Aβ1-42)酶联免疫试剂盒
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货号:CSB-E14398Mk
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规格:96T/48T
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价格:¥4200/¥3000
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其他:
产品详情
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产品描述:本试剂盒(CSB-E14398Mk)采用双抗体夹心法定量检测猴血清或血浆样本中的β淀粉样蛋白1-42(Aβ1-42)含量,适用于神经退行性疾病机制研究及药物开发评估。Aβ1-42作为阿尔茨海默病的核心病理标志物,其异常聚集形成的淀粉样斑块是神经退行性病变的重要特征,在非人灵长类动物模型中具有高度研究价值。试剂盒灵敏度达0.156 ng/mL,线性检测范围为0.156-10 ng/mL,通过预包被特异性捕获抗体与标记检测抗体的协同作用,实现目标蛋白的高特异性识别。实验流程包含样本预处理、抗体孵育、酶标反应及比色测定等标准化步骤,适配常规酶标仪进行数据读取。该产品可广泛应用于阿尔茨海默病模型构建、Aβ代谢通路研究、神经保护药物筛选等科研场景,特别适用于动态监测灵长类动物外周血中Aβ1-42浓度变化,为病理机制解析和干预策略开发提供可靠工具。配套试剂包含标准品、检测抗体及显色底物,支持96孔板规格批量检测。
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别名:Amyloid beta 42(ABeta 42),amyloid beta peptide 1-42,Aβ1-42,amyloid βpeptide 1-42
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缩写:Aβ1-42
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种属:Monkey
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样本类型:serum, plasma
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检测范围:0.156 ng/mL-10 ng/mL
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灵敏度:0.039 ng/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Neuroscience
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 10 2.692 2.789 2.741 2.601 5 2.014 1.997 2.006 1.866 2.5 1.242 1.301 1.272 1.132 1.25 0.679 0.665 0.672 0.532 0.625 0.503 0.500 0.502 0.362 0.312 0.299 0.305 0.302 0.162 0.156 0.245 0.260 0.253 0.113 0 0.141 0.138 0.140 -
数据处理:
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货期:3-5 working days
引用文献
- Correlations between serum levels of beta amyloid, cerebrospinal levels of tau and phospho tau, and delayed response tasks in young and aged cynomolgus Darusman HS et al,J Med Primatol.,2013