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中文名称：小鼠γ分泌酶酶联免疫试剂盒
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货号：CSB-E12142m
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规格：96T/48T
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价格：￥3600/￥2500
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其他：
产品详情
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产品描述：小鼠γ分泌酶酶联免疫试剂盒（CSB-E12142m）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的APH1B含量。APH1B 是一种重要靶点。它在γ-分泌酶复合物中起关键作用。研究发现其与阿尔茨海默病等神经退行性疾病机制相关，可能参与调节淀粉样蛋白生成。目前围绕它探索新治疗策略，深入了解其作用机制或为相关疾病防治带来新思路。试剂盒检测范围为7.8 pg/mL-500 pg/mL，适用于脑组织样本分析、疾病模型构建、神经退行机制探究等科研领域，可支持药物开发中酶活性抑制剂的功效评估、转基因动物模型验证及病理状态下γ分泌酶表达水平动态监测等实验需求，为探索γ分泌酶在细胞信号传导和疾病发生中的功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
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别名：Aph1b; Gamma-secretase subunit APH-1B; APH-1b; Aph-1beta
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缩写：
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Uniprot No.：
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种属：Mus musculus (Mouse)
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样本类型：serum, plasma, tissue homogenates
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检测范围：7.8 pg/mL-500 pg/mL
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灵敏度：1.95 pg/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Neuroscience
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测定原理：quantitative
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测定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of mouse γ-Secretase in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 96 Range % 90-102 1:2 Average % 102 Range % 97-107 1:4 Average % 89 Range % 87-93 1:8 Average % 94 Range % 90-98 -
回收率：
The recovery of mouse γ-Secretase spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 103 99-106 EDTA plasma (n=4) 95 89-102 -
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 500 2.234 2.356 2.295 2.130 250 1.367 1.378 1.373 1.208 125 0.810 0.824 0.817 0.652 62.5 0.492 0.452 0.472 0.307 31.2 0.313 0.306 0.310 0.145 15.6 0.236 0.250 0.243 0.078 7.8 0.205 0.209 0.207 0.042 0 0.160 0.169 0.165 -
数据处理：
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货期：3-5 working days
引用文献
- Pigment Epithelium-Derived Factor Plays a Role in Alzheimer’s Disease by Negatively Regulating AB42 Mao Huang.et al,Neurotherapeutics,2018
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靶点详情
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功能：Probable subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (amyloid-beta precursor protein). It probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex. Probably present in a minority of gamma-secretase complexes compared to APH1A.
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基因功能参考文献：
- Loss of Aph1B is associated with memory deficit. PMID: 26942869
- Prostaglandin I2 accumulation in neuronal cells activates PKA/CREB and JNK/c-Jun signaling pathways by phosphorylation, which results in APH-1alpha/1beta expression. PMID: 27240539
- Abeta oligomers in the cerebrospinal fluid (CSF) further promoted the expression of APH-1alpha/-1beta (by >2.5-fold), which enhances the gamma-cleavage of APP and Abeta deposition during AD progression PMID: 26293690
- analysis of hippocampal circuitry via Aph1b-gamma-secretase/neuregulin 1 signalling PMID: 24891237
- Upregulation of PS1/gamma-secretase activity may be a risk factor for late onset sporadic Alzheimer's disease. PMID: 22140537
- study shows gamma-secretase complexes containing different Aph1 subunits have heterogeneous properties; inactivation of Aph1B gamma-secretase in an Alzheimer disease model led to improvement of disease-relevant features without Notch-related side effects PMID: 19299585
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亚细胞定位：Membrane; Multi-pass membrane protein.
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蛋白家族：APH-1 family
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数据库链接：
