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  4. Mouse 8-Hydroxy-desoxyguanosine,8-OHdG ELISA Kit

Mouse 8-Hydroxy-desoxyguanosine,8-OHdG ELISA Kit

文献引用 (22) 说明书下载
  • 中文名称：
    小鼠8羟基脱氧鸟苷(8-OHdG)酶联免疫试剂盒
  • 货号：
    CSB-E10527m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    小鼠8羟基脱氧鸟苷(8-OHdG)酶联免疫试剂盒（CSB-E10527m）为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的8-OHdG含量。8-OHdG 是 DNA 氧化损伤的生物标志物。其背景在于机体受内外界因素影响产生氧化应激，导致 DNA 中鸟嘌呤氧化形成 8-OHdG。研究机制多围绕检测其含量来评估氧化应激水平、DNA 损伤程度，及与肿瘤、神经退行性疾病等的关联。试剂盒检测范围为0.78 pg/mL-50 pg/mL，适用于神经退行性疾病模型、衰老机制研究、环境毒理学评估及代谢性疾病等科研领域，可为氧化损伤相关机制研究、药物筛选及抗氧化剂功效评价提供数据支持，广泛应用于基础医学、药理学及生物化学等科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 缩写：
    8-OHdG
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.78 pg/mL-50 pg/mL
  • 灵敏度：
    0.195 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse 8-OHdG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 90  
    Range % 87-97  
    1:200 Average % 97  
    Range % 93-104  
    1:400 Average % 85  
    Range % 82-91  
    1:800 Average % 90  
    Range % 85-97  
  • 回收率：
    The recovery of mouse 8-OHdG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 89 85-93  
    EDTA plasma (n=4) 92 88-94  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    50 0.071 0.074 0.073    
    25 0.120 0.130 0.125    
    12.5 0.200 0.206 0.203    
    6.25 0.335 0.340 0.338    
    3.12 0.573 0.554 0.564    
    1.56 0.858 0.841 0.850    
    0.78 1.129 1.139 1.134    
    0 2.215 2.185 2.200    
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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