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  4. Mouse Angiotensin 1-7(Ang1-7)ELISA Kit

Mouse Angiotensin 1-7(Ang1-7)ELISA Kit

文献引用 (15) 说明书下载
  • 中文名称：
    小鼠血管紧张素1-7（Ang1-7）酶联免疫试剂盒
  • 货号：
    CSB-E13763m
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    小鼠血管紧张素1-7（Ang1-7）酶联免疫试剂盒（CSB-E13763m）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的Ang1-7含量。Ang1-7是有研究价值的靶点。它在心血管、肾脏等多个系统发挥作用，具有舒张血管、抗增殖、抗炎等效应。研究机制方面，主要通过与Mas受体结合，激活下游信号通路，调节细胞功能，改善器官病理生理状态。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于实验室对小鼠疾病模型中Ang1-7的动态监测，如高血压、心肌肥厚、肾脏损伤等研究的生物标志物分析，或体外药物筛选实验中细胞因子分泌水平的定量评估。Ang1-7水平变化常被用于心血管疾病模型研究、代谢综合征机制探索及药物干预效果评估。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 缩写：
    Ang1-7
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    6.25 pg/mL-400 pg/mL
  • 灵敏度：
    1.56 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse Ang1-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %91
    Range %86-95
    1:2Average %93
    Range %87-99
    1:4Average %95
    Range %89-102
    1:8Average %95
    Range %87-98
  • 回收率：
    The recovery of mouse Ang1-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9692-98
    EDTA plasma (n=4)9591-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    4002.745 2.721 2.733 2.597
    2002.316 2.245 2.281 2.145
    1001.923 1.898 1.911 1.775
    501.467 1.366 1.417 1.281
    250.796 0.788 0.792 0.656
    12.50.458 0.447 0.453 0.317
    6.250.258 0.242 0.250 0.114
    00.138 0.134 0.136 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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