Mouse C-C motif chemokine 25(CCL25) ELISA kit
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中文名称:小鼠C-C趋化因子25(CCL25)酶联免疫试剂盒
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货号:CSB-EL004789MO
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规格:96T/48T
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价格:¥3600/¥2500
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其他:
产品详情
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产品描述:CUSABIO试剂盒采用双抗体夹心ELISA原理,可定量检测小鼠血清、血浆及组织匀浆中的CCL25蛋白浓度,检测范围为78 pg/ml至5000 pg/ml。C-C趋化因子25(CCL25)属于趋化因子CC亚家族成员,通过与受体CCR9结合参与肠道黏膜免疫调节及淋巴细胞的定向迁移,在肠道相关免疫应答、炎症反应及组织稳态维持中发挥重要作用。本产品通过预包被特异性捕获抗体与标记检测抗体的组合,实现对目标蛋白的高灵敏度定量分析,适用于多种科研场景,例如探索肠道炎症性疾病模型(如结肠炎)中趋化因子的表达调控机制、评估免疫细胞迁移相关信号通路的功能研究,或用于药物开发过程中靶向CCL25/CCR9轴的治疗性化合物体外效果验证。试剂盒操作流程标准化,可兼容常规实验室设备,为研究者提供稳定可靠的数据支持。
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别名:Ccl25 ELISA Kit; Scya25 ELISA Kit; Teck ELISA Kit; C-C motif chemokine 25 ELISA Kit; Chemokine TECK ELISA Kit; Small-inducible cytokine A25 ELISA Kit; Thymus-expressed chemokine ELISA Kit
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缩写:
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Uniprot No.:
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种属:Mus musculus (Mouse)
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样本类型:serum, plasma, tissue homogenates
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检测范围:78 pg/ml - 5000 pg/ml
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灵敏度:19.5 pg/ml
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Immunology
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CCL25 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
101
Range %
98-105
1:2
Average %
99
Range %
96-103
1:4
Average %
89
Range %
85-94
1:8
Average %
97
Range %
92-103
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回收率:
The recovery of mouse CCL25 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
95
91-98
EDTA plasma (n=4)
88
83-92
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标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
5000
2.357
2.366
2.362
2.231
2500
1.825
1.812
1.819
1.688
1250
1.260
1.366
1.313
1.182
625
0.751
0.745
0.748
0.617
312
0.478
0.498
0.488
0.357
156
0.313
0.322
0.318
0.187
78
0.217
0.221
0.219
0.088
0
0.128
0.133
0.131
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数据处理:
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货期:3-5 working days
相关产品
靶点详情
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功能:Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.
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基因功能参考文献:
- these results showed a site- and cell-specific expressions of Ccl25 and its receptor Ccr9 within both maternal and embryonic compartments of implantation sites and suggested a role in inducing specific Ccr9+ leukocytes at the maternal-fetal interface during the initial steps of embryo implantation in the mouse model PMID: 29154408
- MicroRNA-205 maintains T cell development following stress by regulating Foxn1 and its two regulated targets, stem cell factor and ccl25, following stress. PMID: 27646003
- CCR9 and CCL25 expressions are induced in the early stages of airway inflammation and they have an important role modulating eosinophils and lymphocytes recruitment at the first stages of inflammatory process PMID: 27795621
- TECK was shown to be expressed by osteoblasts, and its receptor, CCR9, by osteoclast precursors. TECK increased P. gingivalis LPS-induced osteoclast numbers in an in vitro osteoclast formation assay using osteoclast precursors. T PMID: 26921718
- CCR9/CCL25 is involved in acute skin transplantation rejection and anti-CCL25 strategies might be useful in preventing acute rejection. PMID: 23456208
- Accumulated CD11b(+) macrophages are critical for activating hepatic stellate cells through the CCR9/CCL25 axis and therefore promote liver fibrosis. PMID: 23460364
- The results revealed that during an allergic reaction, CCL25 drives IL-17 gammadelta T-cell mobilization to inflamed tissue via alpha4beta7 integrin and modulates IL-17 levels. PMID: 22539297
- CCL25/CCR9 interactions regulate inflammatory immune responses in the large intestinal mucosa by balancing different subsets of dendritic cells. PMID: 21283540
- Characterization of mouse CCX-CKR, a receptor for the lymphocyte-attracting chemokines TECK/mCCL25, SLC/mCCL21 and MIP-3beta/mCCL19: comparison to human CCX-CKR. (CCX-CKR) PMID: 11981810
- in vivo neutralization of the CCR9 ligand, CCL25, reduced the ability of activated CCR9(+) CD8alphabeta(+) lymphocytes cells to populate the small-intestinal epithelium PMID: 12393847
- role of CCL25 in the generation of the small-intestinal CD8alpha alpha(+)CD3(+) intraepithelial lymphocyte compartment PMID: 12442331
- chemokine (C-C motif) ligand 25 may thus play an important role in the adherence of mucosal lymphocytes to the microvessels of the small intestine but not the colon under uninflamed as well as inflamed conditions PMID: 14592943
- Neonatal CD8+ splenocytes uniformly express alpha(E) integrin and exhibit a high responsiveness to CC chemokine ligand 25. With increasing age, the frequency of CD8+ alpha(E) integrin(+) splenocytes decreases, roughly correlating with thymic involution. PMID: 15187103
- Epithelial cells and venular endothelium of small intestine are immunologically positive for CCL25, a chemokine which plays a direct role in intestinal homing of IgA antibody-secreting cells by mediating their extravasation into intestinal lamina propria. PMID: 15356112
- Chemokine CCL25 enhances CD103-mediated adhesion to E-cadherin. PMID: 15681774
- demonstrate a unique pattern of regulation for CCL25 and suggest a role for caudal type homeo box proteins in regulating CCL25 transcription PMID: 16517733
- Intracellular signaling required for Ccl25-stimulated T cell adhesion mediated by the integrin alpha4beta1. PMID: 17510295
- CCL25 is indispensable for CD8 T cells trafficking to the small intestine. PMID: 17548595
- Demonstrate development of intestinal inflammation in Tnf(DeltaARE) mice is critically dependent on beta7 integrin-mediated T-lymphocyte recruitment. the function of the CCL25/CCR9 axis appears dispensable in this model. PMID: 18439426
- CCL25 increases thymopoiesis after androgen withdrawal PMID: 18694999
- Ovarian CC chemokine thymus-expressed chemokine (TECK) appears to be a chemoattractant for CD8 alpha alpha-positive cells. PMID: 19109193
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亚细胞定位:Secreted.
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蛋白家族:Intercrine beta (chemokine CC) family
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组织特异性:Specifically expressed by thymic dendritic cells. High levels in thymus and small intestine.
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数据库链接: