Mouse Carnitine O-palmitoyltransferase 1, liver isoform(CPT1A) ELISA kit
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中文名称：小鼠肉毒碱-棕榈酰基转移酶1，肝脏亚型(CPT1A)酶联免疫试剂盒
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货号：CSB-EL005922MO
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规格：96T/48T
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价格：￥3600/￥2500
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其他：
产品详情
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产品描述：CUSABIO小鼠肉毒碱-棕榈酰基转移酶1肝脏亚型（CPT1A）酶联免疫检测试剂盒（货号：CSB-EL005922MO），基于双抗体夹心法原理实现定量分析，适用于检测血清、血浆、细胞裂解液及组织匀浆等多种样本中的CPT1A蛋白水平。CPT1A是线粒体脂肪酸β氧化的限速酶，通过调控长链脂肪酸的跨膜转运参与能量代谢调节，其表达水平与肝脏脂质代谢、胰岛素敏感性及代谢性疾病机制研究密切相关。本试剂盒检测灵敏度高，线性范围为6.25-400 pg/mL，可在标准曲线范围内精准测定样本中CPT1A的浓度。该产品可应用于基础医学研究中肝脏代谢功能评估、肥胖或糖尿病动物模型能量代谢通路分析、药物干预对脂肪酸氧化影响的体外实验等领域，为探索脂质代谢调控机制及相关疾病发病机理提供可靠工具。试剂盒包含预包被微孔板、标准品、检测抗体及显色系统等核心组分，操作流程标准化，满足科研实验中对CPT1A蛋白定量检测的需求。
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别名：Cpt1a; Cpt-1; Cpt1; Carnitine O-palmitoyltransferase 1, liver isoform; CPT1-L; Carnitine O-palmitoyltransferase I, liver isoform; CPT I; CPTI-L; Carnitine palmitoyltransferase 1A
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缩写：
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Uniprot No.：
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种属：Mus musculus (Mouse)
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样本类型：serum, plasma, cell lysates, tissue homogenates
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检测范围：6.25 pg/mL-400 pg/mL
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灵敏度：1.56 pg/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Metabolism
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测定原理：quantitative
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测定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CPT1A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 89 Range % 83-100 1:2 Average % 96 Range % 87-102 1:4 Average % 90 Range % 85-99 1:8 Average % 93 Range % 87-99 -
回收率：
The recovery of mouse CPT1A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 97 86-104 EDTA plasma (n=4) 93 89-97 -
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 400 2.006 2.034 2.020 1.843 200 1.372 1.385 1.379 1.202 100 0.735 0.765 0.750 0.573 50 0.431 0.442 0.437 0.260 25 0.286 0.291 0.289 0.112 12.5 0.237 0.248 0.243 0.066 6.25 0.215 0.223 0.219 0.042 0 0.174 0.179 0.177 -
数据处理：
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货期：3-5 working days
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靶点详情
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功能：Catalyzes the transfer of the acyl group of long-chain fatty acid-CoA conjugates onto carnitine, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion. Plays an important role in hepatic triglyceride metabolism.
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基因功能参考文献：
- Alogliptin increased hepatic mRNA expression levels associated with fatty acid oxidation. In addition, the results of the present study suggested that ALO promotes CPT1a expression via Thr172 phosphorylation of AMPKalpha. PMID: 29512720
- Furthermore, given the already low abundance of Cpt1b in white adipose tissue, it is unlikely that decreases in its expression can quantitatively decrease whole body energy expenditure enough to contribute to an obese phenotype. PMID: 28330968
- beneficial effects of muscle CPT1mt expression suggest that a direct modulation of the malonyl-CoA/CPT1 partnership in skeletal muscle could represent a potential strategy to prevent obesity-induced insulin resistance PMID: 27507552
- The data suggest that AMPK is not required for the regulation of the intermediate filament interaction with CPT-I during exercise. PMID: 27941154
- These results suggest that n-3 polyunsaturated fatty acids could improve lipid oxidation-related enzymes in liver subjected to hemorrhagic shock/resuscitation. PMID: 27110821
- these results demonstrated that L-carnitine ameliorated liver inflammation and serum pro-inflammatory markers in cancer cachexia through regulating CPT I-dependent PPARg signaling PMID: 26647854
- Data show that peroxisome-proliferator-activated receptor beta/delta (PPARbeta/delta) activation restored the lipid-induced endothelial dysfunction by up-regulation of carnitine palmitoyltransferase)-1 (CPT-1). PMID: 26253087
- Our results indicated that exercise-induced CPT1b expression was at least in part mediated by HDAC5/MEF2A interaction. PMID: 25213552
- Data indicate that muscle mutated carnitine palmitoyltransferase 1 (CPT1mt) expression enhanced mitochondrial fatty acid oxidation (mFAO) capacity. PMID: 25713059
- Potential epigenetic mechanisms underlying transcriptional regulation of Cpt1alpha in hepatic steatosis occur in response to binge ethanol administration. PMID: 23905631
- an environment-dependent structural switch underlies the regulation of carnitine palmitoyltransferase 1A PMID: 21990363
- miR-370 acting via miR-122 may have a causative role in the accumulation of hepatic triglycerides by modulating initially the expression of SREBP-1c, DGAT2, and Cpt1alpha. PMID: 20124555
- Results show that CREB and HNF4alpha bind the CPT promoter, that PGC-1 is involved in liver carnitine palmitoyltransferase I (CPT) gene activation by cylic AMP, and that PGC-1 acts to coordinate the process of metabolic adaptation in the liver. PMID: 12107181
- role of thyroid hormone response unit formed between the promoter and first intron in mediating liver-specific induction by thyroid hormone PMID: 12493735
- Malonyl-CoA/CPTI interaction is a component of a metabolic signaling network that controls insulin secretion. PMID: 15677504
- modulation of CPT1A expression by PI3K-dependent signaling is the major mechanism by which cells suppress beta-oxidation during anabolic growth PMID: 17030509
- These results suggested that astaxanthin promoted lipid metabolism rather than glucose utilization during exercise via CPT I activation, which led to improvement of endurance and efficient reduction of adipose tissue with training. PMID: 18082622
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亚细胞定位：Mitochondrion outer membrane; Multi-pass membrane protein.
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蛋白家族：Carnitine/choline acetyltransferase family
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数据库链接：
