Mouse Collagen Type Ⅲ,Col Ⅲ ELISA KIT

产品详情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO小鼠Ⅲ型胶原（Col Ⅲ）酶联免疫检测试剂盒（货号：CSB-E07925m），通过双抗体夹心法定量检测血清或血浆样本中的Col Ⅲ蛋白，检测灵敏度达3.12 pg/mL，定量范围为3.12-200 pg/mL。Ⅲ型胶原是细胞外基质核心成分，与Ⅰ型胶原共同构成纤维网络，参与组织修复和纤维化进程，其异常表达与肝纤维化、肺纤维化等病理过程密切相关。CUSABIO试剂盒采用高特异性抗体对靶标进行捕获与检测，适用于小鼠血清或血浆样本的体外定量分析，能够满足纤维化疾病机制研究、药物干预效果评估等科研需求。操作流程标准化，实验耗时约4.5小时，包含预包被微孔板及全套检测组分，支持96孔板规格批量检测，为研究者提供稳定可靠的实验工具。该试剂盒可广泛应用于心血管疾病模型、器官纤维化模型等研究中Col Ⅲ动态变化的监测，为探索纤维化相关分子机制提供数据支持。
别名：

Collagen Type 3,Col 3,_x000D_ Collagen Type III,Col III
缩写：

Col Ⅲ
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma
检测范围：

3.12 pg/mL-200 pg/mL
灵敏度：

0.78 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Col Ⅲ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	89
	Range %	82-96
1:40	Average %	98
	Range %	94-102
1:80	Average %	97
	Range %	90-104
1:160	Average %	86
	Range %	82-90

回收率：

The recovery of mouse Col Ⅲ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-99
EDTA plasma (n=4)	100	96-104

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.