Mouse Collagen Type Ⅰ,Col ⅠELISA Kit 酶联免疫试剂盒-武汉华美生物

Mouse Collagen Type Ⅰ,Col ⅠELISA Kit

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CUSABIO小鼠Ⅰ型胶原（Col Ⅰ）酶联免疫检测试剂盒（货号：CSB-E08083m），采用双抗体夹心法原理，可定量检测血清、血浆、细胞培养上清液及组织匀浆样本中Col Ⅰ的含量，检测灵敏度达0.156 ng/mL，线性范围为0.156-10 ng/mL。Ⅰ型胶原是细胞外基质的主要成分之一，广泛参与组织结构的维持、纤维化进程及损伤修复调控，其表达水平与骨代谢异常、器官纤维化、皮肤修复等病理生理过程密切相关。本试剂盒通过预包被特异性抗体捕获目标蛋白，结合生物素标记的检测抗体及HRP-链霉亲和素系统进行信号放大，最终通过显色反应实现精准定量分析，适用于体外培养细胞模型、动物组织样本或体液中的Col Ⅰ蛋白表达研究，可为纤维化疾病机制探索、骨/软骨再生研究、创伤修复模型构建等科研领域提供可靠数据支持。试剂盒包含标准品、预包被酶标板及检测所需缓冲液组分，操作流程标准化，实验重复性优异，严格遵循科研级质量控制标准。
别名：

Collagen Type 1,Col 1,_x000D_ Collagen Type I,Col I
缩写：

Col Ⅰ
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ColⅠ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	92
	Range %	87-97
1:200	Average %	96
	Range %	91-104
1:400	Average %	97
	Range %	94-101
1:800	Average %	98
	Range %	89-102

回收率：

The recovery of mouse ColⅠ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	98-107
EDTA plasma (n=4)	92	87-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.595	2.494	2.545	2.355
5	1.904	1.803	1.854	1.664
2.5	1.148	1.128	1.138	0.948
1.25	0.660	0.671	0.666	0.476
0.625	0.432	0.442	0.437	0.247
0.312	0.304	0.315	0.310	0.120
0.156	0.249	0.258	0.254	0.064
0	0.189	0.190	0.190

本试剂盒所含材料：
- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-mouse Col I antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled Col I antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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本试剂盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator that can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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产品评价

平均分:

5.0分 - 2 个评价

样品类型：血清

样品信息：小鼠

稀释比：其他 1:10

产品评价：我用CSB-E08083m检测小鼠血清MCAF水平，试剂盒样本量充足，样本和稀释液颜色对应直观，使用说明简洁明了，操作容易上手，数据准确，满意。

By 刘老师

样品类型：血清

样品信息：小鼠

稀释比：其他 1:10

产品评价：我用CSB-E08083m检测小鼠血清胶原蛋白水平，结果如图所示，试剂盒样本充足，样本和稀释液颜色对应直观，使用说明简洁明了，操作容易上手，数据准确，满意。

By 刘老师

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.