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  4. Mouse Corticosterone,CORT ELISA Kit

Mouse Corticosterone,CORT ELISA Kit

文献引用 (39) 评价 (1) 说明书下载
  • 中文名称：
    小鼠皮质酮(CORT)酶联免疫试剂盒
  • 货号：
    CSB-E07969m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    小鼠皮质酮/肾上腺酮(CORT)酶联免疫试剂盒（CSB-E07969m）为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CORT含量。试剂盒检测范围为15.6 ng/ml-1000 ng/ml，适用于体外分析多种生物样本中的CORT水平，尤其适用于评估应激反应模型、慢性代谢疾病研究或药物干预实验中的肾上腺功能状态，可满足实验室对小鼠模型应激水平、激素代谢动态及病理生理机制研究的精准检测需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    N/A
  • 缩写：
    CORT
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 ng/ml-1000 ng/ml.
  • 灵敏度：
    3.9 ng/ml.
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CORT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    84

    Range %

    80-92

    1:2

    Average %

    87

    Range %

    81-95

    1:4

    Average %

    85

    Range %

    82-93

    1:8

    Average %

    88

    Range %

    82-98

  • 回收率：

    The recovery of mouse CORT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    90

    86-98

    EDTA plasma (n=4)

    88

    83-96

  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    1000

    0.150

    0.144

    0.147

    500

    0.185

    0.182

    0.183

    250

    0.261

    0.248

    0.254

    125

    0.389

    0.400

    0.395

    62.5

    0.642

    0.621

    0.632

    31.25

    1.004

    1.053

    1.028

    15.6

    1.410

    1.384

    1.397

    0

    2.166

    2.125

    2.145

  • 本试剂盒所含材料：
      • A micro ELISA plate --- The 96-well plate has been pre-coated with goat anti-rabbit CORT antibody.
      • Six vials lyophilized standard (0.5 ml/bottle) --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • Anti-mouse CORT Antibody (1 x 6 ml) --- Act as the detection antibody.
      • HRP-conjugate (1 x 6 ml) --- HRP catalyzes TMB substrate to elicit a chromogenic reaction.
      • Wash Buffer (20 x concentrate) (1 x 15 ml) --- Wash away unbound or free substances.
      • Substrate A (1 x 7 ml) --- Mix with substrate B, and the TMB is catalyzed by HRP to elicit a chromogenic signal.
      • Substrate B (1 x 7 ml) --- Mix with substrate A, and the TMB is catalyzed by HRP to elicit a chromogenic signal.
      • Stop Solution 1 x 7 ml --- Stop the color development, and the eventual solution color turns yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microwell plate when incubation.
      • An Instruction manual

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  • 本试剂盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm to 630 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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产品评价

平均分:
5.0分 - 1 个评价

样品类型：血清

样品信息：小鼠

稀释比：其他 1:25

产品评价： 使用CSB-E07969m试剂盒检测小鼠血清CORT水平，稀释25倍后大部分均在标曲范围内，但如果样品充足可考虑1:10稀释。全部实验在3.5小时内完成，检测结果较稳定，标曲良好.

By 兰老师

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靶点详情

  • 最新研究进展：
    CORT是一种主要由肾上腺皮质合成的类固醇激素，具有重要的生理功能，例如调节葡萄糖代谢、免疫系统和心血管系统等。最新研究表明，CORT在多种疾病的发生和发展中也发挥着重要作用。例如，CORT与焦虑和抑郁等心理疾病的发病机制有关。此外，一些研究还发现CORT在自身免疫性疾病、肿瘤、神经系统疾病和心血管疾病等方面发挥着重要作用。

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