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  4. Mouse Eotaxin 2 ELISA Kit

Mouse Eotaxin 2 ELISA Kit

文献引用 (1) 说明书下载
  • 中文名称：
    小鼠嗜酸粒细胞趋化蛋白Eotaxin 2(Eotaxin 2/CCL24)酶联免疫试剂盒
  • 货号：
    CSB-E07278m
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：
    CUSABIO小鼠嗜酸粒细胞趋化蛋白Eotaxin 2（CCL24）酶联免疫检测试剂盒（货号：CSB-E07278m），采用双抗体夹心法原理实现定量分析，适用于科研领域对血清、血浆及组织匀浆样本中CCL24蛋白含量的精准检测。Eotaxin 2作为CC趋化因子家族成员，在嗜酸粒细胞募集及过敏反应、哮喘等炎症性疾病中发挥关键调控作用，其动态监测对免疫机制研究具有重要意义。该试剂盒检测灵敏度达15.6 pg/ml，线性范围覆盖15.6-1000 pg/ml，可在标准实验条件下稳定检测低丰度目标蛋白，为探索炎症相关疾病的分子机制、评估药物干预效果或构建疾病模型提供可靠工具。实验操作兼容常规ELISA流程，适用于体外培养细胞上清、动物模型体液及组织样本的CCL24表达水平分析，满足科研人员对趋化因子网络调控研究的多元化需求。
  • 别名：
    Ccl24 ELISA Kit; Scya24 ELISA Kit; C-C motif chemokine 24 ELISA Kit; Eosinophil chemotactic protein 2 ELISA Kit; Eotaxin-2 ELISA Kit; Small-inducible cytokine A24 ELISA Kit
  • 缩写：
    CCL24
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/ml - 1000 pg/ml
  • 灵敏度：
    3.9 pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse eotaxin 2/CCL24 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    89

    Range %

    80-96

    1:2

    Average %

    94

    Range %

    91-105

    1:4

    Average %

    96

    Range %

    92-99

    1:8

    Average %

    93

    Range %

    86-98

  • 回收率：

    The recovery of mouse eotaxin 2 /CCL24 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    95

    89-98

    EDTA plasma (n=4)

    97

    94-101

  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    1000

    2.612

    2.634

    2.623

    2.507

    500

    2.200

    2.267

    2.233

    2.117

    250

    1.453

    1.528

    1.491

    1.374

    125

    0.753

    0.729

    0.741

    0.625

    62.5

    0.349

    0.329

    0.339

    0.222

    31.2

    0.242

    0.253

    0.248

    0.131

    15.6

    0.180

    0.184

    0.182

    0.066

    0

    0.113

    0.120

    0.116

    　

  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

产品评价

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Ccl24 Proteins

靶点详情

  • 功能：
    Chemotactic for resting T-lymphocytes, and eosinophils. Has lower chemotactic activity for neutrophils but none for monocytes and activated lymphocytes. Is a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. Binds to CCR3.
  • 基因功能参考文献：
    1. the increased airway eosinophils and mucous cell metaplasia in house dust mite-challenged Cd163-/- mice are mediated by augmented CCL24 production PMID: 26376364
    2. this study shows that TPL-2 inhibits Ccl24 expression in lung dendritic cells, and blockade of Ccl24 prevents the exaggerated airway eosinophilia and lung inflammation in mice given house dust mites-pulsed Map3k8-/- dendritic cells PMID: 27484038
    3. production in M-CSF-induced bone marrow-derived macrophages is synergistically induced by IL-4 and IL-10 PMID: 25267883
    4. Autologous transfer of peritoneal macrophages in to the airways of asthmatic mice reduces eotaxin production. PMID: 24077949
    5. Data indicate that eotaxin 2 (CCL-24), which is known to influence eosinophil migration, was highly up-regulated in broncho-alveolar macrophages (BAMs). PMID: 23685352
    6. The oesophageal production of CCL24 upon IL-13 stimulation is sufficient to promote eosinophil migration. PMID: 20030665
    7. Results demonstrate a cooperative mechanism between interleukin-13 and eotaxin-2, where IL-13 mediates allergen-induced eotaxin-2 expression, and eotaxin-2 mediates IL-13-induced airway eosinophilia. PMID: 15647285
    8. Distinct acidic and basic residues within CCR3 determine both receptor expression and activation by the eotaxins. PMID: 16102831
    9. The eotaxin-2 pathway plays a fundamental role in eosinophil recruitment during ovalbumin-induced experimental asthma. PMID: 16210640
    10. eosinophils via chemokine (C-C) receptor 3 have a central role in chronic allergic airway disease PMID: 17060636
    11. CCL24 seems important for recruitment of eosinophils into BAL. PMID: 18850374

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine beta (chemokine CC) family
  • 组织特异性：
    Highest expression in jejunum and spleen. Lower levels found in liver and lung. No expression detected in kidney, thymus, brain or testis.
  • 数据库链接：

    KEGG: mmu:56221

    STRING: 10090.ENSMUSP00000004936

    UniGene: Mm.31505

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