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  4. Mouse Epinephrine/Adrenaline,EPI ELISA Kit

Mouse Epinephrine/Adrenaline,EPI ELISA Kit

文献引用 (15) 说明书下载
  • 中文名称：
    小鼠肾上腺素(EPI)酶联免疫试剂盒
  • 货号：
    CSB-E08679m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO试剂盒采用竞争性酶联免疫吸附法，专为定量检测小鼠血清、血浆及组织匀浆中的肾上腺素（EPI）而设计。肾上腺素作为肾上腺髓质分泌的关键激素，参与调节机体应激反应、能量代谢及心血管功能，是神经内分泌研究的重要靶标。其检测范围覆盖0.78-50 ng/ml，通过样本中的EPI与酶标抗原竞争结合固相抗体的原理实现定量分析：EPI浓度越高，显色信号越弱，通过标准曲线可精准计算目标物含量。该试剂盒适用于多种科研场景，包括应激生理学模型中激素动态变化的监测、药物或环境因素对交感-肾上腺轴影响的评估，以及代谢性疾病或心血管研究中EPI调控机制的探索。试剂盒严格验证了血浆和匀浆样本的兼容性，操作流程稳定高效，可为神经生物学、药理学及基础医学研究提供可靠的数据支持，助力于激素相关分子机制的解析。
  • 缩写：
    EPI
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates.
  • 检测范围：
    0.78 ng/ml-50 ng/ml.
  • 灵敏度：
    0.2 ng/ml.
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse EPI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    84

    Range %

    80-92

    1:2

    Average %

    87

    Range %

    85-100

    1:4

    Average %

    85

    Range %

    82-99

    1:8

    Average %

    89

    Range %

    86-98

  • 回收率：

    The recovery of mouse EPI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    85

    82-90

    EDTA plasma (n=4)

    87

    83-96

  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    50

    0.180

    0.174

    0.177

    25

    0.307

    0.317

    0.312

    12.5

    0.485

    0.508

    0.496

    6.25

    0.750

    0.729

    0.740

    3.12

    1.228

    1.301

    1.265

    1.56

    1.972

    1.953

    1.963

    0.78

    2.565

    2.484

    2.524

    0

    2.916

    2.825

    2.871

  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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