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Mouse Factor-related Apoptosis,FAS ELISA Kit

  • 中文名称:
    小鼠凋亡相关因子(FAS/CD95)酶联免疫试剂盒
  • 货号:
    CSB-E04543m
  • 规格:
    96T/48T
  • 价格:
    ¥3200/¥2500
  • 其他:

产品详情

  • 产品描述:
    CUSABIO定量检测小鼠血清、血浆、细胞培养上清液或组织匀浆样本中凋亡相关因子(FAS/CD95)的酶联免疫试剂盒,采用双抗体夹心法原理,检测灵敏度达0.625 ng/mL,线性范围为0.625-40 ng/mL,可精准分析样本中FAS/CD95蛋白的动态变化。FAS/CD95是肿瘤坏死因子受体超家族成员,通过介导细胞凋亡信号参与免疫调节、组织稳态维持及病理过程,其异常表达与自身免疫疾病、肿瘤发生及神经退行性疾病密切相关。该试剂盒采用高特异性配对抗体,支持多种生物样本类型检测,适用于体外细胞凋亡机制研究、药物干预效果评估或动物模型中FAS信号通路的动态监测,为探索细胞程序性死亡调控机制、免疫微环境分析及靶向治疗开发提供可靠工具,实验全程无需特殊设备,操作流程标准化,重复性良好。
  • 别名:
    Fas; Apt1; Tnfrsf6; Tumor necrosis factor receptor superfamily member 6; Apo-1 antigen; Apoptosis-mediating surface antigen FAS; FASLG receptor; CD antigen CD95
  • 缩写:
  • Uniprot No.:
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围:
    0.625 ng/mL-40 ng/mL
  • 灵敏度:
    0.156 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cell Biology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse FAS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:1 Average % 104
    Range % 97-108
    1:2 Average % 106
    Range % 97-110
    1:4 Average % 94
    Range % 85-97
    1:8 Average % 100
    Range % 92-104
  • 回收率:
    The recovery of mouse FAS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 103 95-107
    EDTA plasma (n=4) 100 89-104
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    40 2.965 2.848 2.907 2.737
    20 2.658 2.592 2.625 2.455
    10 2.166 2.079 2.123 1.953
    5 1.502 1.478 1.490 1.320
    2.5 0.832 0.844 0.838 0.668
    1.25 0.551 0.543 0.547 0.377
    0.625 0.329 0.319 0.324 0.154
    0 0.172 0.168 0.170  
  • 数据处理:
  • 货期:
    3-5 working days

产品评价

靶点详情

  • 功能:
    Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both.
  • 基因功能参考文献:
    1. our computational and experimental approach identified Fas as a regulator of the Th17-to-Th1 cell balance by controlling the availability of opposing STAT1 and STAT3 to have a direct impact on autoimmunity. PMID: 29562202
    2. FGF21 alleviated atherosclerosis by ameliorating Fas-mediated apoptosis in apoE-/- mice. PMID: 30157856
    3. TPD7 altered the extrinsic apoptosis pathway by upregulating Fas expression. PMID: 29901176
    4. In conclusion, these data demonstrate that murine herpesvirus 68-immortalized SL-1 cells can be recognized and controlled by specific cytotoxic T cells through CD95/CD95L-mediated apoptosis. PMID: 28516317
    5. Findings indicate that induction of apoptosis through Fas is dependent on receptor palmitoylation in primary immune cells, and Fas may prevent autoimmunity by mechanisms other than inducing apoptosis. PMID: 28008916
    6. Both Sharpin/Fas and Sharpin/Fasl compound mutant mice developed an auto-inflammatory phenotype similar to that seen in Sharpin null mice, indicating that initiation of apoptosis by FAS signalling is likely not involved in the pathogenesis of this disease. PMID: 28094869
    7. Tag7 activates lymphocytes capable of Fasl-Fas-dependent contact killing of virus-infected cells. PMID: 29083508
    8. leucine deprivation induces the expression of miR-212-5p in a GCN2/ATF4-dependent manner. miR-212-5p suppresses lipid accumulation in liver by targeting FAS and SCD1 under both normal diet and high-fat diet conditions. PMID: 28667176
    9. Our data show that loss of Fas activity strongly affects the early development of atopic dermatitis (AD) by leading to Th2-dominant inflammation characterized by dermal infiltration of CD4+ T cells, neutrophils and increased skin expression of Th2 cytokines.However, Fas/FasL-apoptotic pathway is also involved in restricting tissue remodelling and dermal fibrosis during AD. PMID: 28434120
    10. Hrd1-null B cells exhibited high Fas expression during activation and rapidly underwent Fas-mediated apoptosis, which could be largely inhibited by FasL neutralization. Fas mutation in Hrd1 KO mice abrogated the increase in B-cell AICD. We identified Hrd1 as the first E3 ubiquitin ligase of the death receptor Fas and Hrd1-mediated Fas destruction as a molecular mechanism in regulating B-cell immunity. PMID: 27573825
    11. FAS contributes to mitochondrial dysfunction, steatosis development, and insulin resistance under high fat diet. PMID: 28883393
    12. These findings reveal a role for MOAP-1 in Fas signaling in the liver by promoting MTCH2-mediated tBid recruitment to mitochondria. PMID: 27320914
    13. The in vivo delivery of CRISPR/Cas9 could maintain liver homeostasis and protect hepatocytes from Fas-mediated cell apoptosis in the fulminant hepatic failure model. PMID: 27585307
    14. This study demonstrated that Ischemic neurons release sFasL, which contributes to M1-microglial polarization. PMID: 27283206
    15. results indicate that IL-1beta, produced by the inflammasome and Fas-dependent mechanisms, contributes cooperatively to the Th17/Th1 induction during bacterial infection. This study provides a deeper understanding of the molecular mechanisms underlying Th17/Th1 induction during pathogenic microbial infections in vivo. PMID: 28674179
    16. this study shows that CD95-mediated calcium signaling promotes Th17 cell trafficking to inflamed organs in lupus-prone mice PMID: 27438772
    17. accelerating effects of Tlr9 deficiency PMID: 28278279
    18. K8/K18-dependent PKCdelta- and ASMase-mediated modulation of lipid raft size can explain the more prominent FasR-mediated signaling resulting from K8/K18 loss. PMID: 27422101
    19. Data show that TCF1 proteindeficiency relieved most manifestations of autoimmune lymphoproliferative syndrome (ALPS)-like phenotype, which were caused by Fas protein mutation in TCF1(-/-) lpr/lpr mice. PMID: 28349581
    20. Results indicate that the close interaction between Thy-1 and Fas in lipid rafts regulates fibroblast apoptosis, and decreased fibroblast apoptosis associated with myofibroblast accumulation in mice lacking Thy-1. PMID: 28165468
    21. Fas/FasL Complex Promotes Proliferation and Migration of Brain Endothelial Cells Via FADD-FLIP-TRAF-NF-kappaB Pathway PMID: 25427888
    22. Cardiac Fas-dependent and mitochondria-dependent apoptotic pathways were activated in transgenic mice with Huntington's disease. PMID: 25800750
    23. The MWM showed that compared with FAS- and FASL-knockout mice treated with sevoflurane, sevoflurane treatment of wild-type mice significantly prolonged the escape latency and reduced platform crossing times. PMID: 26782453
    24. the individual functions of the NF-kappaB family members NF-kappaB1, NF-kappaB2 and c-REL in the various autoimmune pathologies of Fas(lpr/lpr) mutant mice, were investigated. PMID: 26084385
    25. When Bax(-/-)Bak(-/-) murine embryonic stem cells (ESCs) are stimulated to differentiate, a subpopulation fails to do so and instead upregulates FAS in a p53-dependent manner to trigger Bax/Bak-dependent apoptosis. PMID: 26585277
    26. These results demonstrate that during ectromelia virus infection, Fas/FasL can regulate development of tolerogenic DCs and Tregs, leading to an ineffective immune response. PMID: 26780774
    27. Data determined the transmembrane domain structure of Fas and showed that the trimer assembly, which is mediated by a proline-containing motif, is essential for Fas signaling providing structural explanation for many known cancer mutations in this domain. PMID: 26853147
    28. Impaired Fas-Fas Ligand Interactions Result in Greater Recurrent Herpetic Stromal Keratitis in Mice PMID: 26504854
    29. miR-150 deficiency prevents Fas-induced hepatocyte apoptosis and liver injury through regulation of the Akt pathway PMID: 26196694
    30. CD47 deficiency ameliorates lupus nephritis in Fas(lpr) mice via suppression of IgG autoantibody production. PMID: 26095930
    31. The upregulation of p-FADD/FADD ratio and NF-kappaB in mouse hippocampus after Kainic acid treatment PMID: 26044520
    32. demonstrates that Fas/FasL pathway during ectromelia virus infection of the lungs plays an important role in controlling local inflammatory response and mounting of antiviral response PMID: 25873756
    33. Mice with the Fas(lpr) gene developed severe systemic lupus erythematosus with renal dysfunction and inflammatory responses in the lung and kidney. By contrast, mice with the Fas(+) gene showed disease-related abnormalities in the liver and joints. PMID: 25941813
    34. Occlusive lung arterial lesions triggering pulmonary arterial hypertension developed in a new model of endothelial-targeted, Fas-induced apoptosis transgenic mice. PMID: 25879383
    35. Gene silencing of liver Fas expression completely attenuated apoptotic and necrotic cell death. PMID: 25601293
    36. Our results demonstrate that Fas/FasL can regulate development of tolerogenic dendritic cells and expansion of Tregs early during HSV-2 infection, which further influences effective anti-viral response. PMID: 25129477
    37. our data support a model in which IFNgamma- and Fas/FasL-dependent activation of intratumoral Mvarphis by CD8(+) T cells promotes severe intraocular inflammation that indirectly eliminates intraocular tumors by inducing phthisis. PMID: 25248763
    38. Meningococcal capsular polysaccharide-loaded vaccine nanoparticles induce expression of CD95. PMID: 24981893
    39. These data provide the first in vivo genetic evidence that neutrophil lifespan is controlled by death receptor signaling and provide a mechanism to account for neutrophil resistance to Fas stimulation during infection. PMID: 25473101
    40. Intestinal expression of Fas and Fas ligand is upregulated by bacterial signaling through TLR4 and TLR5, with activation of Fas modulating intestinal TLR-mediated inflammation. PMID: 25378591
    41. overexpression of Fas/FasL is associated with infectious complications and severity of experimental severe acute pancreatitis by promoting apoptosis of lymphocytes PMID: 24566874
    42. No significant association between FAS-670G/A polymorphism and susceptibility to autoimmune hepatitis was found. PMID: 24629822
    43. a key role of MK2 and FasR in the regulation and limitation of the immune response in the CNS PMID: 24964076
    44. These data show that loss of Fas activity specifically in chondrocytes prolonged the life span of chondrocytes and that Fas synergized with TNFalpha signaling to mediate chondrocyte apoptosis. PMID: 24677136
    45. Although expression of Fas and TNF-R1 was proportionate to fractional apoptosis, cell death was dominated by spontaneous apoptosis in stem cell mobilization. PMID: 24566711
    46. Data suggest that toll-like receptor 3 (TLR3), phosphatidylinositol 3-kinase (PI3K), survivin, Fas ligand (FasL), and CD95 (Fas) genes are involved in the development of cervical cancer. PMID: 25106857
    47. The Fas KO mice spontaneously develop blepharitis with not only autoimmune inflammation with deposition of auto-antibody but also allergic inflammation with infiltration by eosinophils and show to increase serum level of IgE and IgG1. PMID: 23220580
    48. D-cyclins repress the expression of the death receptor Fas and its ligand, FasL PMID: 25087893
    49. Data indicate that dendritic cells (DCs)-specific CD95 (Fas) expression plays a role in regulation of antiviral responses and suggests a strategy for stimulation of T cells for virus clearance in chronically infected animal and human. PMID: 24912151
    50. Combined adenovirus-mediated artificial microRNAs targeting mfgl2, mFas, and mTNFR1 protect against fulminant hepatic failure in mice. PMID: 24303082

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  • 相关疾病:
    Defects in Fas are the cause of the lymphoproliferation phenotype (lpr). Lpr mice show lymphadenopathy and autoantibody production.
  • 亚细胞定位:
    Cell membrane; Single-pass type I membrane protein. Membrane raft.
  • 组织特异性:
    Detected in various tissues including thymus, liver, lung, heart, and adult ovary.
  • 数据库链接:

    KEGG: mmu:14102

    STRING: 10090.ENSMUSP00000025691

    UniGene: Mm.1626