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  4. Mouse Interleukin-19(IL19) ELISA kit

Mouse Interleukin-19(IL19) ELISA kit

说明书下载
  • 中文名称：
    小鼠白介素19(IL19)酶联免疫试剂盒
  • 货号：
    CSB-EL011612MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    小鼠白介素19(IL19)酶联免疫试剂盒（CSB-EL011612MO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL19含量。IL19 属 IL-10 细胞因子家族成员。其在炎症与免疫调节等方面发挥重要作用。研究机制上，它能结合特异性受体，激活下游信号通路，参与调控细胞的增殖、分化以及炎症因子的释放，目前是相关疾病治疗靶点研究的热点。试剂盒检测范围为12.5 pg/mL-800 pg/mL，支持科研领域对小鼠模型中IL19表达动态的研究，例如炎症性疾病机制探索、药物干预效果评估或免疫调控通路分析，为体外实验提供可靠的定量数据支持，但不适用于临床诊断或体内检测用途。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Il19Interleukin-19 ELISA Kit; IL-19 ELISA Kit
  • 缩写：
    IL19
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    12.5 pg/mL-800 pg/mL
  • 灵敏度：
    3.12 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL19 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 107    
    Range % 96-112    
    1:2 Average % 103    
    Range % 94-107    
    1:4 Average % 95    
    Range % 86-99    
    1:8 Average % 100    
    Range % 90-104    
  • 回收率：
    The recovery of mouse IL19 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 102 88-106    
    EDTA plasma (n=4) 103 92-107    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    800 2.707 2.656 2.682 2.542    
    400 1.494 1.544 1.519 1.379    
    200 0.829 0.845 0.837 0.697    
    100 0.422 0.446 0.434 0.294    
    50 0.309 0.313 0.311 0.171    
    25 0.201 0.212 0.207 0.067    
    12.5 0.176 0.171 0.174 0.034    
    0 0.142 0.138 0.140 　    
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

产品评价

相关产品

Il19 Proteins

靶点详情

  • 功能：
    May play some important roles in inflammatory responses. Up-regulates IL-6 and TNF-alpha and induces apoptosis.
  • 基因功能参考文献：
    1. Data, including data from studies using knockout mice, suggest that interleukin-19 suppresses hapten-dependent skin inflammation in elicitation phase of contact hypersensitivity. PMID: 29386478
    2. Attenuated colitis in IL-19-deficient animals was associated with reduced numbers of IL-6-producing macrophages in the inflamed colonic lamina propria. Microbial-driven expression of IL-19 by intestinal macrophages may contribute to the pathogenesis of inflammatory bowel disease. PMID: 28864472
    3. IL-19 can promote angiogenesis in the absence of hypoxia by at least two distinct mechanisms: 1) direct effects on vascular cells and 2) indirect effects by stimulation of macrophages. PMID: 27053520
    4. IL-19 plays an anti-inflammatory role in the Th2-mediated colitis model. PMID: 27942772
    5. microglia may self-limit their inflammatory response by producing the negative regulator IL-19. PMID: 25794104
    6. Lack of IL-19 exacerbates intimal hyperplasia PMID: 24814101
    7. we delineate the detailed molecular pathway for IL-4 up-regulation of IL-19 in keratinocytes, which may play an important role in AD pathogenesis. PMID: 24943510
    8. Il-19 expression is increased in astrocytes following bacterial challenge. PMID: 24677051
    9. IL-19 is important for cutaneous wound healing because it upregulates KGF expression. PMID: 23582717
    10. IL-19 is a potent inhibitor of experimental atherosclerosis. PMID: 23950143
    11. IL-19 is pivotal in the pathogenesis of breast cancer. PMID: 22186257
    12. endogenous IL-19 is a constituent of the regulome that controls both processes in vivo PMID: 22110701
    13. IL-19 drives pathogenic innate immune responses in the colon. PMID: 19834971
    14. In a dust mite-induced murine model of asthma, the IL-19 level in asthmatic BALB/cJ mice is twice that of healthy control mice. PMID: 15557163

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    IL-10 family
  • 数据库链接：

    KEGG: mmu:329244

    STRING: 10090.ENSMUSP00000016668

    UniGene: Mm.131480

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