Mouse Interleukin 35(IL-35) ELISA Kit

产品详情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

小鼠白介素35(IL-35)酶联免疫试剂盒（CSB-E13145m）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IL-35含量。IL-35是IL-12家族的新成员，由EBI3和p35亚基组成。它在免疫调节中发挥关键作用，与IL12B形成IL-12细胞因子或与EBI3/IL27B形成IL-35细胞因子。IL-35在多种炎症和肿瘤中表达异常，其作用机制涉及调节T细胞和自然杀伤细胞反应，以及诱导干扰素γ的产生。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于体外培养细胞因子分泌分析、实验动物模型血清/组织IL-35动态监测等场景；为研究IL-35在免疫调控机制、疾病模型中的功能解析及药物干预效果评价提供了高效工具，广泛应用于免疫学、肿瘤学及炎症相关基础研究领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

IL-35
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-35 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	87-97
1:2	Average %	95
	Range %	89-99
1:4	Average %	94
	Range %	90-100
1:8	Average %	93
	Range %	87-97

回收率：

The recovery of mouse IL-35 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	87-98
EDTA plasma (n=4)	96	91-101

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.