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  4. Mouse Keratinocyte Growth Factor(KGF/FGF-7) ELISA Kit

Mouse Keratinocyte Growth Factor(KGF/FGF-7) ELISA Kit

文献引用 (1) 说明书下载
  • 中文名称：
    小鼠角化细胞生长因子(KGF/FGF-7)酶联免疫试剂盒
  • 货号：
    CSB-E13046m
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO小鼠角化细胞生长因子（KGF/FGF-7）酶联免疫检测试剂盒（货号CSB-E13046m），采用双抗体夹心法原理实现定量检测，适用于分析血清、血浆、细胞培养上清及组织匀浆等多种样本类型。KGF/FGF-7属于成纤维细胞生长因子家族，特异性作用于上皮细胞，在皮肤修复、黏膜再生及组织稳态调控中发挥重要作用。试剂盒检测灵敏度低至1.56 pg/mL，线性范围覆盖1.56-100 pg/mL，通过预包被特异性捕获抗体和酶标记检测抗体的协同作用，确保数据的精确性和重复性。该产品可广泛应用于上皮细胞生物学、组织再生机制、伤口愈合模型等基础研究领域，也可用于评估药物干预或基因修饰对KGF表达水平的影响。对于细胞实验，可直接检测培养上清中分泌型KGF的浓度；针对动物模型，支持从血清、血浆或特定组织匀浆中定量分析内源性KGF的表达动态，为皮肤、肺、肠道等上皮组织相关研究提供可靠工具。
  • 别名：
    Fgf7 ELISA Kit; Fgf-7 ELISA Kit; Kgf ELISA Kit; Fibroblast growth factor 7 ELISA Kit; FGF-7 ELISA Kit; Heparin-binding growth factor 7 ELISA Kit; HBGF-7 ELISA Kit; Keratinocyte growth factor ELISA Kit; KGF ELISA Kit
  • 缩写：
    FGF7
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    1.56 pg/mL-100 pg/mL
  • 灵敏度：
    0.39 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse KGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %94
    Range %89-98
    1:200Average %96
    Range %91-102
    1:400Average %90
    Range %85-96
    1:800Average %94
    Range %85-105
  • 回收率：
    The recovery of mouse KGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9185-96
    EDTA plasma (n=4)10095-103
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    1002.513 2.569 2.541 2.349
    502.163 2.168 2.166 1.974
    251.633 1.658 1.646 1.454
    12.50.992 0.999 0.996 0.804
    6.250.562 0.571 0.567 0.375
    3.120.368 0.383 0.376 0.184
    1.560.272 0.291 0.282 0.090
    00.190 0.193 0.192 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

产品评价

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Fgf7 Proteins

靶点详情

  • 功能：
    Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation.
  • 基因功能参考文献：
    1. Tregs express kgf. Treg-specific expression of kgf regulates alveolar epithelial proliferation during the resolution phase of acute lung injury and in a model of regenerative alveologenesis in vivo. AT2 cells cocultured with Tregs lacking kgf have decreased rates of proliferation. PMID: 28296468
    2. A role for endogenous KGF in maintenance of normal pulmonary innate immune function. PMID: 26919897
    3. Suppression of miR-219-5p may benefit the liver regeneration and prevent cirrhosis through increasing KGF. PMID: 27855391
    4. we examine how the two presynaptic organizers FGF22 and FGF7 are transported to the appropriate locations so that they act as synapse-type-specific organizing molecules. PMID: 25431136
    5. Exendin-4 increased fibroblast growth factor 7 (Fgf7) expression in colonic polyps of Apc(Min/+) mice. PMID: 25738454
    6. Our observations also point towards PI3K acting as a morphogenic switch for FGF7 signalling. PMID: 25460003
    7. Reverse transcriptase polymerase chain reaction studies showed that three members, Tgfa, Hbegf,and Nrg1 of the EGF family were expressed in the epithelium cultured with FGF7 + LPA as well as in the epithelium freshly isolated from the rudiments. PMID: 24227310
    8. IL-19 is important for cutaneous wound healing because it upregulates KGF expression. PMID: 23582717
    9. FGF7 is downregulated in MED1-deficient mesenchymal cells. FGF7 produced by mesenchymal stromal cells is a novel hematopoietic niche molecule. PMID: 24051090
    10. KGF pretreatment could improve radiation-induced intestinal injury including the epithelial structure and function in a mouse model, as shown by Zo-1 protein and Claudin-1. PMID: 23464848
    11. KGF could up-regulate IL-7 expression through the STAT1/IRF-1, IRF-2 signaling pathway, which is a new insight in potential effects of KGF on the intestinal mucosal immune system. PMID: 23554911
    12. Fibroblast growth factor 7 (Fgf7) was identified as a target of miR-143 in murine adipogenesis. PMID: 23247565
    13. FGF7 is a critical regulator of liver progenitor cells PMID: 23322300
    14. Given the specifically targeted effects of FGF-7 on epithelial cells, the authors' findings suggest that further FGF-7-dependent epithelial-mesenchymal interaction exists that may be important in diabetic wound healing. PMID: 22094769
    15. Keratinocyte growth factor gene transduction ameliorates pulmonary fibrosis induced by bleomycin in mice PMID: 21131445
    16. These results suggest that KGF may play an important role in the regulation of VEGF gene expression and angiogenesis during wound healing. PMID: 21672345
    17. KGF promotes preadipocyte proliferation by an autocrine mechanism that involves activation of the PI3K/Akt signaling pathway PMID: 20069574
    18. two members of the fibroblast growth factor (FGF) family, FGF22 and FGF7, promote the organization of excitatory and inhibitory presynaptic terminals, respectively, as target-derived presynaptic organizers PMID: 20505669
    19. keratinocyte growth factor works via an inducible lentivirus to protect bone marrow cells against bleomycin-induced pulmonary fibrosis PMID: 19956603
    20. Protection from thymic epithelial cell injury by keratinocyte growth factor PMID: 12036893
    21. Keratinocyte growth factor preserves normal thymopoiesis and thymic microenvironment during experimental graft-versus-host disease. PMID: 12091365
    22. Following activation by KGF binding, KGF and the KGF receptor remain associated in active complexes through the endocytic pathway, which is described. PMID: 12122441
    23. Stress-induced alterations in cutaneous proinflammatory cytokine and growth factor expression are associated with significant histological changes in healing wounds. PMID: 12161023
    24. gammadelta cell-deficient mice (TCRdelta(-/-)) and KGF-deficient mice (KGF(-/-)) were more prone than wild-type mice to DSS-induced mucosal injury and demonstrated delayed tissue repair after termination of DSS treatment PMID: 12376619
    25. PAK4 interacts with KGF receptor and mediate anti-apoptosis effects of KGF on epithelial cells. PMID: 12529371
    26. In a mouse model of total parenteral nutrition (TPN), application of KGF to TPN mice reversed the decline of proliferation of EC during TPN and prevented the increase in EC apoptosis with TPN alone. PMID: 12592627
    27. KGF-induced Akt activation may play an important role in inhibiting lung alveolar cell death thereby preserving the lung architecture and function during oxidative stress PMID: 12732722
    28. BMP4 and FGF7/FGF10 arrest early T-cell development at the CD4-CD8-CD44+CD25- (double-negative 1 [DN1]) population and at the CD4-CD8- double-negative to CD4+CD8+ double-positive transition in a stromal compartment-dependent manner PMID: 12920023
    29. Structure-based mutational analyses in FGF7 identify new residues involved in specific interaction with FGFR2IIIb. PMID: 14527678
    30. KGF from gamma delta-T cell receptor-expressing intraepithelial lymphocytes is an important factor for maintenance of intestinal epithelial cell proliferation and villus growth. PMID: 15034027
    31. Biological activity of FGF-7 in RAG-1 mice indicates that immuno-dependent mechanisms are not required for the induction of urothelial cell proliferation by this growth factor. PMID: 15176316
    32. epithelial Wnt11, mesenchymal Gdnf and stromal Fgf7 signalling is coordinated by sprouty proteins during kidney development, which regulates ureteric branching PMID: 15201220
    33. KGF antibody abolished the growth promoting effect of GLP-2 in the large intestine, but not in the small intestine PMID: 15544847
    34. KGF appears to be an important growth factor for local resident progenitor epithelial cell repair PMID: 17332441
    35. FGF7 improved survival during acute lung injury in adult mouse lung after short-term expression, but paradoxically induced inflammation and injury after persistent expression. PMID: 17994369
    36. Krt12-rtTA/tet-O-FGF-7 mice may be a suitable animal model for the study of the molecular and cellular mechanisms of human ocular surface squamous neoplasia. PMID: 18276784
    37. Ectopic expression of KGF in acinar cells promotes the expansion of the beta-cell lineage in vivo through activation of the PKB/Akt pathway. PMID: 18372394
    38. p63/p51-induced onset of keratinocyte differentiation via the c-Jun N-terminal kinase pathway is counteracted by keratinocyte growth factor PMID: 18849344
    39. Fgf7 protects against acute lung injury PMID: 17994369

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Heparin-binding growth factors family
  • 数据库链接：

    KEGG: mmu:14178

    STRING: 10090.ENSMUSP00000069681

    UniGene: Mm.330557

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