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中文名称：小鼠粘蛋白/粘液素5AC(MUC5AC)酶联免疫试剂盒
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货号：CSB-E11040m
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规格：96T/48T
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价格：￥3200/￥2500
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其他：
产品详情
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产品描述：小鼠粘蛋白/粘液素5AC(MUC5AC)酶联免疫试剂盒（CSB-E11040m）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的MUC5AC含量。MUC5AC是一个重要靶点。它是一种黏蛋白，与呼吸系统等疾病密切相关，如哮喘、慢性阻塞性肺疾病等。研究机制主要聚焦于其在疾病发生发展中参与调节黏液分泌、影响炎症反应等，对它的深入研究有望为相关疾病治疗提供新策略。试剂盒检测范围为4.688 ng/mL-300 ng/mL，本试剂盒适用于体外实验研究，如评估病理状态下粘液分泌调节机制、筛选影响粘蛋白表达的化合物或药物，以及探究感染性疾病中宿主防御反应等科研领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
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缩写：
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种属：Mus musculus (Mouse)
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样本类型：serum, plasma, cell culture supernates, tissue homogenates
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检测范围：4.688 ng/mL-300 ng/mL
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灵敏度：1.172 ng/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Signal Transduction
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测定原理：quantitative
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测定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of mouse MUC5AC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 87 Range % 82-92 1:2 Average % 90 Range % 85-95 1:4 Average % 98 Range % 91-102 1:8 Average % 96 Range % 93-103 -
回收率：
The recovery of mouse MUC5AC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 98 91-104 EDTA plasma (n=4) 95 90-100 -
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 300 2.275 2.182 2.229 2.057 150 1.463 1.434 1.449 1.277 75 0.855 0.818 0.837 0.665 37.5 0.524 0.507 0.516 0.344 18.75 0.351 0.329 0.340 0.168 9.375 0.281 0.262 0.272 0.100 4.688 0.235 0.217 0.226 0.054 0 0.174 0.169 0.172 -
数据处理：
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货期：3-5 working days
引用文献
- Discovery of Fungus-Derived Nornidulin as a Novel TMEM16A Inhibitor: A Potential Therapy to Inhibit Mucus Secretion in Asthma P Pongkorpsakol,Journal of experimental pharmacology,2023
- Gleditsia sinensis Lam. aqueous extract attenuates nasal inflammation in allergic rhinitis by inhibiting MUC5AC production through suppression of the STAT3/STAT6 pathway MA Jung,Biomedicine & pharmacotherapy,2023
- Alnus hirsuta (Spach) Rupr. Attenuates Airway Inflammation and Mucus Overproduction in a Murine Model of Ovalbumin-Challenged Asthma Ji-Hye Ha,Frontiers in Pharmacology,2021
- Combined treatment with SB203580 and dexamethasone suppresses non-typeable Haemophilus influenzae-induced Th17 inflammation response in murine allergic asthma Wang G, et al,European Journal of Pharmacology,2019
- Chronic intermittent hypoxia decreases pulmonary clearance of 99mTc-labelled particulate matter in mice Fu C.et al,Am J Transl Res,2017
- The immune profile associated with acute allergic asthma accelerates clearance of influenza virus Samarasinghe AE et al,Immunol Cell Biol,2014
- Role of the Adiponectin Binding Protein, T-Cadherin (Cdh13), in Allergic Airways Responses in Mice /,PLOS ONE,2012
- A Dissociated Glucocorticoid Receptor Modulator Reduces Airway Hyperresponsiveness and Inflammation in a Mouse Model of Asthma /,The Journal of Immunology,2012
产品评价
平均分:
样品类型：组织匀浆液 肺组织匀浆上清
样品信息：小鼠
稀释比：没有稀释
产品评价： 我通过使用CSB-E11040m检测了PR8病毒感染的小鼠肺组织匀浆上清，操作简单方便易上手，灵敏度非常高，检测结果十分精准
By 邓老师
样品类型：组织匀浆液 肺组织匀浆上清
样品信息：小鼠
稀释比：没有稀释
产品评价： 我通过使用CSB-E11040m检测了PR8病毒感染的小鼠肺组织匀浆上清，操作简单方便易上手，灵敏度非常高，检测结果十分精准
By 邓老师
样品类型：组织匀浆液 肺组织匀浆上清
样品信息：小鼠
稀释比：1:100
产品评价： 我通过使用CSB-E11040m检测了流感病毒感染的小鼠肺组织匀浆上清，操作简单方便易上手，灵敏度非常高，检测结果十分精准
By 邓老师
