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  4. Mouse Neuron-specific enolase,NSE ELISA Kit

Mouse Neuron-specific enolase,NSE ELISA Kit

文献引用 (2) 说明书下载
  • 中文名称：
    小鼠神经特异性烯醇化酶(NSE)酶联免疫试剂盒
  • 货号：
    CSB-E07962m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    小鼠神经特异性烯醇化酶(NSE)酶联免疫试剂盒（CSB-E07962m）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的ENO2含量。ENO2作为靶点，它是烯醇化酶的一种。ENO2在神经组织及神经内分泌来源的细胞中特异性表达，在肿瘤发生发展等过程可能发挥重要作用。其研究机制主要围绕探究其在细胞代谢、增殖、凋亡等方面的分子调控作用，以寻找潜在治疗靶点和干预手段。试剂盒检测范围为1.56 ng/mL-100 ng/mL，适用于神经损伤模型评估、神经细胞分化研究、神经相关疾病机制探索以及药物干预效果监测等科研领域，为神经科学基础研究提供稳定可靠的工具支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Eno2 ELISA Kit; Eno-2Gamma-enolase ELISA Kit; EC 4.2.1.11 ELISA Kit; 2-phospho-D-glycerate hydro-lyase ELISA Kit; Enolase 2 ELISA Kit; Neural enolase ELISA Kit; Neuron-specific enolase ELISA Kit; NSE ELISA Kit
  • 缩写：
    ENO2
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, cell culture supernates
  • 检测范围：
    1.56 ng/mL-100 ng/mL
  • 灵敏度：
    0.39 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse NSE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %98
    Range %89-102
    1:2Average %91
    Range %87-95
    1:4Average %94
    Range %84-97
    1:8Average %91
    Range %85-94
  • 回收率：
    The recovery of mouse NSE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10592-108
    EDTA plasma (n=4)9184-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    1002.622 2.542 2.582 2.475
    502.209 2.167 2.188 2.081
    251.463 1.487 1.475 1.368
    12.50.762 0.734 0.748 0.641
    6.250.358 0.333 0.346 0.239
    3.120.252 0.245 0.249 0.142
    1.560.180 0.168 0.174 0.067
    00.106 0.108 0.107
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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Eno2 Proteins

靶点详情

  • 功能：
    Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
  • 基因功能参考文献：
    1. Study analyzed the regions that regulate the promoter strength and cellular specificity of the 1.8 kb rNSE promoter in the cerebellum in vivo. The rNSE promoter was divided into three regions (A, B, and C). Our results showed that region C was indispensable for the promoter activity in mouse cerebellum and served as an interneuron-predominant basal promoter. PMID: 28508170
    2. Plaque-associated microglial cells express gamma-enolase and cathepsin X. PMID: 23621429
    3. Treadmill exercise training decreased the expression of NSN in the striatum of chronic Parkinsonian mice, which can partially explain the beneficial neuroprotective role of exercise in patients with parkinson disease. PMID: 21725169
    4. we induced differentiation of mouse stromal cells to neuron-like cells and observed the activation of tissue-specific promoter of neuron-specific enolase in transgenic mice with the human bcl-2 gene expressed only in neurons under the NSE promoter PMID: 14593212
  • 亚细胞定位：
    Cytoplasm. Cell membrane.
  • 蛋白家族：
    Enolase family
  • 组织特异性：
    Skeletal muscle (at protein level). The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodi
  • 数据库链接：

    KEGG: mmu:13807

    STRING: 10090.ENSMUSP00000004378

    UniGene: Mm.3913

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