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  4. Mouse Noradrenaline,NA ELISA Kit

Mouse Noradrenaline,NA ELISA Kit

文献引用 (28) 说明书下载
  • 中文名称：
    小鼠去甲肾上腺素(NA)酶联免疫试剂盒
  • 货号：
    CSB-E07870m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    小鼠去甲肾上腺素(NA)酶联免疫试剂盒（CSB-E07870m）为竞争法ELISA试剂盒，定量检测血清、血浆样本中的NA含量。小鼠去甲肾上腺素（NA）作为一种重要的神经递质，在中枢神经系统和交感神经系统中参与调节生理过程，如感觉信号、注意力、清醒与睡眠、学习与记忆等。其释放或信号传递的受损与精神疾病和神经退行性疾病相关。近年来，研究者开发了新型基因编码的NA荧光探针，通过实时监测NA动态变化，为研究NA相关神经环路提供了重要工具。试剂盒检测范围为7.5 pg/mL-300 pg/mL，本试剂盒适用于神经生物学研究、应激反应机制探索、心血管药理学实验中对NA分泌水平的动态监测，也可用于评估抗高血压药物或神经调节剂对NA代谢通路的影响。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    N/A
  • 缩写：
    NA
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma
  • 检测范围：
    7.5 pg/mL-300 pg/mL
  • 灵敏度：
    7.5 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse noradrenaline in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 100  
    Range % 98-104  
    1:2 Average % 87  
    Range % 84-90  
    1:4 Average % 86  
    Range % 83-89  
    1:8 Average % 91  
    Range % 88-94  
  • 回收率：
    The recovery of mouse noradrenaline spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 85 82-89  
    EDTA plasma (n=4) 97 94-100  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    300 0.371 0.396 0.384    
    150 0.548 0.578 0.563    
    69 0.775 0.798 0.787    
    22.5 1.126 1.168 1.147    
    7.5 1.672 1.587 1.630    
    0 2.201 2.209 2.205    
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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