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  4. Mouse Osteocrin(OSTN) ELISA kit

Mouse Osteocrin(OSTN) ELISA kit

文献引用 (1) 说明书下载
  • 中文名称：
    小鼠骨织素(OSTN)酶联免疫试剂盒
  • 货号：
    CSB-EL017269MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    小鼠骨织素(OSTN)酶联免疫试剂盒（CSB-EL017269MO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的OSTN含量。OSTN即骨钙素，是一种分泌型骨细胞因子。它在骨骼发育、矿化等过程中发挥重要作用。研究机制上，其能调节成骨细胞与破骨细胞的活动，影响骨代谢平衡，还可参与能量代谢、糖脂代谢等，有望成为骨代谢疾病等领域的治疗靶点。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于能量代谢研究、骨发育调控机制探索、运动生理学实验等科研领域。可广泛应用于肥胖模型、骨质疏松症研究或生物节律相关实验中的OSTN动态监测，为体外培养细胞或动物模型中的OSTN表达分析提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Ostn ELISA Kit; Osteocrin ELISA Kit; Musclin) [Cleaved into: Processed Osteocrin] ELISA Kit
  • 缩写：
    OSTN
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Stem Cells
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse OSTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:5Average %92
    Range %87-97
    1:10Average %95
    Range %88-99
    1:20Average %93
    Range %87-100
    1:40Average %95
    Range %87-104
  • 回收率：
    The recovery of mouse OSTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9485-99
    EDTA plasma (n=4)9789-105
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.579 2.605 2.592 2.502
    5001.447 1.494 1.471 1.381
    2500.791 0.803 0.797 0.707
    1250.430 0.427 0.429 0.339
    62.50.219 0.225 0.222 0.132
    31.20.144 0.149 0.147 0.057
    15.60.107 0.112 0.110 0.020
    00.088 0.091 0.090
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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Ostn Proteins

相关问答

■ 常见问题解答
Q:

Is an extraction step required from Plasma?

A:
Thanks for your inquiry! Extraction step is not required from Plasma.

靶点详情

  • 功能：
    Hormone that acts as a ligand for natriuretic peptide receptor NPR3/NPR-C and promotes bone growth and physical endurance in muscle. Acts as a regulator of osteoblast differentiation and bone growth by binding to natriuretic peptide receptor NPR3/NPR-C, thereby preventing binding between NPR3/NPR-C and natriuretic peptides, leading to increase cGMP production. Required to enhance physical endurance: induced following physical exercise in muscle and promotes cGMP production, probably by interacting with NPR3/NPR-C. May act as an autocrine and paracrine factor linked to glucose metabolism in skeletal muscle.
  • 基因功能参考文献：
    1. The mechanism underlying the effect of OSTN on skeletal growth relevant to C-type natriuretic peptide /Natriuretic peptide receptor B signaling. PMID: 28990933
    2. Data show that musclin (Ostn) production is stimulated by Ca2+-dependent proto-oncogene protein c-Akt (Akt) phosphorylation. PMID: 26668395
    3. findings suggest that palmitate increases musclin gene expression via the activation of the PERK signaling pathway in C2C12 myotubes PMID: 26449458
    4. osteocrin represents a novel, unique vitamin D-regulated bone-specific protein that appears to act as a soluble osteoblast regulator PMID: 14523025
    5. Musclin expression level is tightly regulated by nutritional changes and its physiological role could be linked to glucose metabolism PMID: 15044443
    6. Foxo1 downregulates musclin mRNA expression both in vitro and in vivo, which should explain insulin-mediated upregulation of this gene in muscle cells. PMID: 17950246
    7. Ostn is a naturally occurring ligand of the NPR-C clearance receptor and may act to locally modulate the actions of the natriuretic system in bone by blocking the clearance action of NPR-C, thus locally elevating levels of C-type natriuretic peptide. PMID: 17951249

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Osteocrin family
  • 组织特异性：
    Expressed in skeletal muscle and to a much lesser extent in bone, brown adipose tissue, spleen and testis. Not expressed in neurons.
  • 数据库链接：

    KEGG: mmu:239790

    UniGene: Mm.390880

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