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  4. Mouse Protein Wnt-16(WNT16) ELISA kit

Mouse Protein Wnt-16(WNT16) ELISA kit

文献引用 (1) 说明书下载
  • 中文名称：
    小鼠Wnt-16蛋白(WNT16)酶联免疫试剂盒
  • 货号：
    CSB-EL026132MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    小鼠Wnt-16蛋白(WNT16)酶联免疫试剂盒（CSB-EL026132MO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的WNT16含量。小鼠Wnt-16蛋白（WNT16）在骨骼稳态中起关键作用，其功能缺失会导致骨密度降低和骨折风险增加。WNT16通过调控成骨细胞功能影响骨骼发育，其调控机制涉及内含子2区域的启动子活性与CPED1增强子区域的物理互作，可能影响骨骼稳态及骨质疏松症治疗。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于小鼠模型或细胞实验中Wnt - 16蛋白表达水平的功能研究，例如探索骨骼发育异常机制、肿瘤微环境信号传导或干细胞定向分化调控等科研场景；适用于基础研究与药物开发中Wnt信号通路的分子机制探索本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Wnt16 ELISA Kit; Protein Wnt-16 ELISA Kit
  • 缩写：
    WNT16
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    62.5 pg/mL-4000 pg/mL
  • 灵敏度：
    15.6 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Stem Cells
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse WNT16 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %103
    Range %101-105
    1:2Average %96
    Range %93-99
    1:4Average %89
    Range %86-92
    1:8Average %98
    Range %94-102
  • 回收率：
    The recovery of mouse WNT16 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 106101-111
    EDTA plasma (n=4)9289-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    40002.379 2.465 2.422 2.268
    20002.022 1.970 1.996 1.842
    10001.450 1.560 1.505 1.351
    5000.865 0.899 0.882 0.728
    2500.531 0.527 0.529 0.375
    1250.375 0.383 0.379 0.225
    62.50.288 0.278 0.283 0.129
    00.158 0.150 0.154
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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Wnt16 Proteins

靶点详情

  • 功能：
    Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
  • 基因功能参考文献：
    1. A large number of Mef2c targets overlapped with genes down-regulated by Wnt16 and Mef2c itself was transcriptionally repressed by Wnt16 suggesting that Mef2c plays a role in Wnt16-mediated transcriptional regulation. PMID: 29981832
    2. that WNT16 plays a critical role for acquisition of both cortical and trabecular bone mass and strength PMID: 28013361
    3. WNT16 antagonises excessive canonical WNT activation and protects cartilage in osteoarthritis. PMID: 27147711
    4. Wnt16 Is Associated with Age-Related Bone Loss and Estrogen Withdrawal in Murine Bone PMID: 26451596
    5. Describe TGFbeta-Wnt16-Notch signaling conduit in the chondrocyte-like transformation of VSMCs and identify endogenous TGFbeta activity in MGP-null VSMCs as a critical mediator of chondrogenesis. PMID: 25614285
    6. Wnt5a abrogated the inhibitory effects of Wnt16 on Rankl-induced osteoclastogenesis PMID: 26093292
    7. These findings suggest that WNT16 acting via canonical WNT signaling regulates mechanical strain-induced periosteal bone formation and bone size. PMID: 25521583
    8. Osteoblast-derived WNT16 is a previously unreported key regulator of osteoclastogenesis and fracture susceptibility. PMID: 25306233
    9. Wnt16 is involved in intramembranous ossification and suppresses osteoblast differentiation through the Wnt/beta-catenin pathway. PMID: 24037946
    10. Study revealed new domains of expression for Wnt2, Wnt2b, Wnt5b, Wnt6, Wnt7b, Wnt9a, Wnt10a, Wnt10b, Wnt11 and Wnt16, in the limb. PMID: 19185060

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  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    Wnt family
  • 数据库链接：

    KEGG: mmu:93735

    STRING: 10090.ENSMUSP00000031681

    UniGene: Mm.137403

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