Mouse Scavenger receptor cysteine-rich type 1 protein M130(CD163) ELISA kit
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中文名称：小鼠清道夫受体富含半胱氨酸1蛋白M130(CD163)酶联免疫试剂盒
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货号：CSB-EL004882MO
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规格：96T/48T
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价格：￥3600/￥2500
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其他：
产品详情
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产品描述：CUSABIO针对小鼠清道夫受体富含半胱氨酸1蛋白M130（CD163）开发的酶联免疫检测试剂盒（货号：CSB-EL004882MO），采用双抗体夹心法定量分析血清、血浆及组织匀浆样本中的CD163含量。CD163作为特异性表达于单核/巨噬细胞表面的血红蛋白清除受体，在炎症调控、氧化应激反应及红细胞代谢过程中发挥重要作用，是研究巨噬细胞功能及相关疾病机制的关键分子。试剂盒检测灵敏度高，定量检测范围为0.93 ng/mL至60 ng/mL，最低检测限达0.23 ng/mL，可兼容不同稀释比例的样本（血清/血浆推荐1:20稀释，组织匀浆建议1:5稀释后离心取上清）。该试剂盒适用于炎症模型研究、免疫调控机制探索、血液相关疾病动物实验等科研场景，能够有效评估巨噬细胞活化状态或病理条件下CD163的动态变化，为心血管疾病、代谢综合征及感染性疾病的基础研究提供可靠工具，实验全程约需4.5小时完成。
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别名：Cd163 ELISA kit; M130Scavenger receptor cysteine-rich type 1 protein M130 ELISA kit; CD antigen CD163) [Cleaved into: Soluble CD163 ELISA kit; sCD163)] ELISA kit
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缩写：
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Uniprot No.：
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种属：Mus musculus (Mouse)
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样本类型：serum, plasma, tissue homogenates
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检测范围：0.9 ng/ml-60 ng/ml
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灵敏度：0.23 ng/ml
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Immunology
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测定原理：quantitative
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测定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CD163 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
97
Range %
91-103
1:2
Average %
87
Range %
80-96
1:4
Average %
87
Range %
84-91
1:8
Average %
95
Range %
91-99
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回收率：
The recovery of mouse CD163 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
88
82-95
EDTA plasma (n=4)
92
87-96
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标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml
OD1
OD2
Average
Corrected
60
2.306
2.233
2.270
2.206
30
1.356
1.275
1.316
1.252
15
0.742
0.752
0.747
0.683
7.5
0.481
0.491
0.486
0.422
3.75
0.264
0.275
0.270
0.206
1.8
0.207
0.205
0.206
0.142
0.9
0.105
0.100
0.103
0.039
0
0.063
0.065
0.064
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数据处理：
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货期：3-5 working days
产品评价
相关产品
靶点详情
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最新研究进展：CD163是一种单核细胞的受体，可参与调节细胞的炎症和免疫反应。最新研究表明，CD163在多种疾病的发生发展中起重要作用，如肝病、炎症性肠病、肺炎、骨质疏松等。此外，CD163还可能成为肿瘤治疗的新靶点，相关研究正在进行中。
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功能：Involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1.; After shedding, the soluble form (sCD163) may play an anti-inflammatory role.
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基因功能参考文献：
- house dust mite -challenged Cd163-/- mice displayed increases in airway eosinophils and mucous cell metaplasia PMID: 26376364
- In diabetic mice, increased sCD163 at wk 5 and decreased percentage of CD163(+) monocytes at wk 10 preceded alteration in kidney collagen IV mRNA at wk 20. In vitro incubation of monocytes in anti-inflammatory glucocorticoid increased the percentage of CD163(+) monocytes. PMID: 27354410
- During ischaemia, soluble CD163 functions as a decoy receptor for TWEAK, to regulate TWEAK-induced activation of canonical nuclear factor-kappaB and Notch signalling necessary for myogenic progenitor cell proliferation. PMID: 26242746
- Data indicate an essential substrate motif for ADAM17-mediated CD163 and proTNF-alpha cleavage in macrophages. PMID: 24275664
- alpha(1)-Acid glycoprotein up-regulates CD163 via TLR4/CD14 protein pathway: possible protection against hemolysis-induced oxidative stress. PMID: 22807450
- Data show that telmisartan reduced the mRNA expression of CD11c and TNF-alpha, M1 macrophage markers, and significantly increased the expressions of M2 markers, such as CD163, CD209. PMID: 21427223
- Assignment of the CD163 antigen to mouse chromosome 6 band F2. PMID: 12698011
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亚细胞定位：[Soluble CD163]: Secreted.; Cell membrane; Single-pass type I membrane protein.
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组织特异性：Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen and mesenteric lymph nodes.
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数据库链接：
