-
中文名称：小鼠β淀粉样蛋白1-40(Aβ1-40)酶联免疫试剂盒
-
货号：CSB-E08300m
-
规格：96T/48T
-
价格：￥3800/￥2500
-
其他：
产品详情
-
产品描述：CUSABIO小鼠β淀粉样蛋白1-40（Aβ1-40）酶联免疫检测试剂盒（货号CSB-E08300m），采用双抗体夹心法原理进行定量检测，适用于科研领域对血清、血浆及组织匀浆样本中Aβ1-40蛋白含量的精准分析。Aβ1-40作为β淀粉样蛋白的重要亚型，其异常沉积与神经退行性病变密切相关，是阿尔茨海默病发病机制研究的关键生物标志物。试剂盒检测灵敏度为31.25 pg/mL，线性检测范围覆盖31.25-2000 pg/mL，可稳定检测样本中痕量Aβ1-40的表达变化。该产品通过预包被高特异性捕获抗体实现目标蛋白富集，配合标记检测抗体形成复合物进行显色定量，实验流程包含标准品梯度稀释、样本孵育、洗涤及显色步骤，操作标准化程度高。主要应用于神经科学领域的基础研究，包括阿尔茨海默病小鼠模型建立、药物干预后Aβ代谢通路评估、神经毒性作用机制探索等方向，为病理机制解析和潜在治疗策略开发提供可靠数据支持。
-
别名：amyloid beta peptide 1-40,Aβ1-40,amyloid β peptide 1-40
-
缩写：Aβ1-40
-
种属：Mus musculus (Mouse)
-
样本类型：serum, plasma, tissue homogenates
-
检测范围：31.25 pg/mL-2000 pg/mL
-
灵敏度：7.8 pg/mL
-
反应时间：1-5h
-
样本体积：50-100ul
-
检测波长：450 nm
-
研究领域：Neuroscience
-
测定原理：quantitative
-
测定方法：Sandwich
-
精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of mouse Aβ1-40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 89 Range % 80-97 1:2 Average % 95 Range % 90-105 1:4 Average % 95 Range % 83-100 1:8 Average % 93 Range % 83-98 -
回收率：
The recovery of mouse Aβ1-40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 97 93-106 EDTA plasma (n=4) 89 86-92 -
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 2000 2.637 2.739 2.688 2.512 1000 2.115 2.092 2.104 1.928 500 1.318 1.287 1.303 1.127 250 0.824 0.810 0.817 0.641 125 0.491 0.504 0.498 0.322 62.5 0.322 0.315 0.319 0.143 31.25 0.265 0.273 0.269 0.093 0 0.172 0.180 0.176 -
数据处理：
-
货期：3-5 working days
引用文献
- A Novel Missense Variant in SORBS2 Is Causative With Familial Alzheimer's Disease Q Wang, S Wang, S Cao, Q Wang, Y Wei,CNS Neuroscience & Therapeutics,2025
- Inhibition of Amyloid-β (Aβ)-Induced Cognitive Impairment and Neuroinflammation in CHI3L1 Knockout Mice through Downregulation of ERK-PTX3 Pathway HJ Ham,International journal of molecular sciences,2024
- Yuanzhi Powder Facilitated the Aβ Clearance in App/Ps1 Mice: Target to the Drainage of Glymphatic System and Meningeal Lymphatic Vessels J Li,Available at SSRN 4528275,2023
- Aberrant palmitoylation caused by a ZDHHC21 mutation contributes to pathophysiology of Alzheimer's disease W Li,BMC medicine,2023
- TNIP2 Inhibits Amyloidogenesis by Regulating the 3'UTR of BACE1 L Wang,Neuroscience letters,2023
- TNIP2 inhibits amyloidogenesis by regulating the 3'UTR of BACE1: an in vitro study L Chen,Neuroscience letters,2023
- Persimmon leaf extract alleviates chronic social defeat stress-induced depressive-like behaviors by preventing dendritic spine loss via inhibition of serotonin … H Yu,Chinese medicine,2022
- Hippocampal CysLT1R overexpression or activation accelerates memory deficits, synaptic dysfunction, and amyloidogenesis in young APP/PS1 transgenic mice SC Fang,Annals of translational medicine,2021
- Dimethyl fumarate abridged tauo-/amyloidopathy in a D-Galactose/ovariectomy-induced Alzheimer's-like disease: Modulation of AMPK/SIRT-1, AKT/CREB/BDNF, AKT/GSK-3β, Adiponectin/Adipo1R, and NF-κB/IL-1β/ROS trajectories IM Abd El-Fatah,Neurochemistry International,2021
- Dysregulated APP expression and α-secretase processing of APP is involved in manganese-induced cognitive impairment Y Yang,Ecotoxicology and Environmental Safety,2021
产品评价
相关产品
相关问答
■ 常见问题解答
Q:
Hi I would like to ask the following for the item Mouse amyloid beta peptide 1-40 ELISA kits:
1. How may test samples can be performed in 1 kit, is it possible to use the kit for 1 96-well plate
A:
Thanks for your inquiry. This is a sandwich ELISA kit (double-antibody). For a 96T sandwich ELISA kit, we suggest you run duplicates for the standard. In this way you can test 80 samples. We also suggest you run duplicates for the sample, and in this way you can test 40 samples. You can decide how many kits you need according to the number of your samples. If you run a pre-test, the number of samples you can detect in one kit will be less.
