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  4. Mouse amyloid beta peptide 1-42,Aβ1-42 ELISA Kit

Mouse amyloid beta peptide 1-42,Aβ1-42 ELISA Kit

文献引用 (36) 说明书下载
  • 中文名称：
    小鼠β淀粉样蛋白1-42(Aβ1-42)酶联免疫试剂盒
  • 货号：
    CSB-E10787m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO定量检测小鼠血清、血浆及组织匀浆中β淀粉样蛋白1-42（Aβ1-42）的酶联免疫试剂盒，基于双抗体夹心原理开发，适用于神经退行性疾病相关科研领域。Aβ1-42作为阿尔茨海默病的关键病理标志物，其异常沉积与神经元损伤密切相关，该试剂盒通过高灵敏度抗体对实现15.6-1000 pg/mL的宽范围检测，能够精准量化样本中Aβ1-42的动态变化。实验体系包含预包被捕获抗体和生物素标记检测抗体，通过链霉亲和素-HRP显色系统实现目标蛋白定量分析，操作流程标准化且重复性良好。其多样本兼容性支持研究者同时分析循环系统（血清/血浆）与脑组织等局部病灶中的蛋白表达差异，适用于阿尔茨海默病小鼠模型构建、Aβ病理机制研究、药物干预效果评估等实验场景，为神经科学领域提供可靠的检测工具。本产品严格验证批次稳定性，实验全程无需特殊设备，配套标准品及缓冲液确保实验便捷性，满足体外科研检测需求。
  • 别名：
    Amyloid beta 42(ABeta 42),amyloid beta peptide 1-42,Aβ1-42,amyloid βpeptide 1-42
  • 缩写：
    Aβ1-42
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse Aβ1-42 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 88-99  
    1:2 Average % 95  
    Range % 85-105  
    1:4 Average % 96  
    Range % 90-105  
    1:8 Average % 93  
    Range % 86-100  
  • 回收率：
    The recovery of mouse Aβ1-42 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 90-100  
    EDTA plasma (n=4) 93 85-96  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1000 2.490 2.451 2.471 2.337  
    500 2.049 1.985 2.017 1.883  
    250 1.357 1.385 1.371 1.237  
    125 0.866 0.837 0.852 0.718  
    62.5 0.517 0.524 0.521 0.387  
    31.2 0.347 0.355 0.351 0.217  
    15.6 0.244 0.228 0.236 0.102  
    0 0.135 0.132 0.134 　  
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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■ 常见问题解答
Q:

Hi I would like to ask the following for the item Mouse amyloid beta peptide 1-40 ELISA kits:
1. How may test samples can be performed in 1 kit, is it possible to use the kit for 1 96-well plate

A:
Thanks for your inquiry. This is a 96T sandwich ELISA kit (double-antibody). For a 96T sandwich ELISA kit we suggest you run duplicates for the standard. In this way you can test 80 samples. We also suggest you run duplicates for the sample, and in this way you can test 40 samples. You can decide how many kits you need according to the number of your samples. If you run a pre-test, the number of samples you can detect in one kit will be less.

靶点详情

  • 最新研究进展：
    Aβ1-42是β淀粉样蛋白（Aβ）家族的一员，它是阿尔茨海默病（AD）病理学的主要成分。最近的研究表明，Aβ1-42的积聚是导致AD的主要因素之一。已经发现许多抗Aβ1-42药物，如免疫疗法和β-淀粉样蛋白药物，这些药物能够清除Aβ1-42，并恢复AD患者的认知能力。此外，一些研究还发现，Aβ1-42可能是癫痫的重要病理因素，这为癫痫治疗提供了新的思路。

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