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Mouse calcitonin gene related peptide (CGRP) ELISA kit

  • 中文名称:
    小鼠降钙素基因相关肽(CGRP)酶联免疫试剂盒
  • 货号:
    CSB-EQ027706MO
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

产品详情

  • 产品描述:
    CUSABIO小鼠降钙素基因相关肽(CGRP)酶联免疫检测试剂盒(货号:CSB-EQ027706MO),基于双抗体夹心ELISA原理开发,适用于定量检测小鼠血清、血浆或组织匀浆样本中的CGRP水平。CGRP是一种重要的神经肽类物质,广泛分布于中枢及外周神经系统,参与调节血管舒张、神经源性炎症和痛觉信号传递等生理病理过程,在偏头痛、心血管疾病及神经退行性疾病研究中具有重要价值。该试剂盒检测范围为15.6 pg/mL至1000 pg/mL,通过优化抗体对实现高特异性结合,能够精准识别内源性CGRP分子。实验操作包含标准品梯度稀释和样本预处理步骤,支持科研人员探究CGRP在神经科学、药理学或疾病模型中的动态变化,例如分析疼痛模型中神经递质释放规律、评估药物对CGRP通路的调控作用,或研究心血管疾病与神经肽关联机制。本试剂盒适用于多种生物样本类型,为神经生物学、免疫学及代谢性疾病等基础研究领域提供标准化检测工具。
  • 别名:
    Beta type CGRP、Calcitonin gene-related peptide II、CGRPII、Calcitonin gene-related peptide 2、CALCB
  • 缩写:
  • Uniprot No.:
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    15.6 pg/mL-1000 pg/mL
  • 灵敏度:
    3.9 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Signal Transduction
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CGRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 94  
    Range % 86-98  
    1:2 Average % 92  
    Range % 88-97  
    1:4 Average % 90  
    Range % 85-96  
    1:8 Average % 94  
    Range % 88-99  
  • 回收率:
    The recovery of mouse CGRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 89-97  
    EDTA plasma (n=4) 96 91-99  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1000 2.342 2.321 2.332 2.178  
    500 1.772 1.760 1.766 1.612  
    250 1.250 1.241 1.246 1.092  
    125 0.799 0.789 0.794 0.640  
    62.5 0.499 0.487 0.493 0.339  
    31.25 0.364 0.341 0.353 0.199  
    15.6 0.253 0.244 0.249 0.095  
    0 0.156 0.152 0.154    
  • 数据处理:
  • 货期:
    3-5 working days

产品评价

平均分:
5.0分 - 2 个评价

样品类型:组织匀浆液 小鼠神经节组织

样品信息:小鼠

稀释比:没有稀释

产品评价: ELISA试剂盒在检测CGRP时展现了较高的灵敏度和特异性。在小鼠drg样本中,重复性良好。试剂盒的操作简便,且厂家提供了详细的实验步骤和在线技术支持,帮助解决了实验中的问题。ELISA试剂盒的检测范围(最低和最高浓度)也适应实验的需求。具备高灵敏度,能够在低浓度下准确检测CGRP。

By 闫老师

样品类型:组织匀浆液 小鼠肺组织匀浆液

样品信息:小鼠

稀释比:1:50

产品评价: 我使用CSB-EQ027706MO检测小鼠肺组织匀浆液样本,标准曲线的 OD 值在 0.176 - 1.749 之间呈现良好线性关系,样本 OD 值大致在 0.2 - 1.7 区间。试剂盒的重复性较好,多次平行实验结果波动比较小。从操作体验来说,试剂预分装设计十分贴心,减少了繁琐的准备步骤,节省了时间。而且灵敏度较高,能精准检测到CGRP,为科研数据提供了可靠支撑,是一款非常值得信赖的 ELISA 试剂盒。

By 王老师

靶点详情

  • 功能:
    CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role.
  • 亚细胞定位:
    Secreted.
  • 蛋白家族:
    Calcitonin family
  • 组织特异性:
    Detected in nerve cells of cerebrum, hippocampus and pons/midbrain in newborns, and only in nerve cells of pons/midbrain in adult.
  • 数据库链接: