Mouse glucagon-like peptide-1,GLP-1 ELISA Kit 酶联免疫试剂盒-武汉华美生物

Mouse glucagon-like peptide-1,GLP-1 ELISA Kit

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产品描述：

CUSABIO小鼠胰高血糖素样肽1（GLP-1）酶联免疫检测试剂盒（货号：CSB-E08118m），采用双抗体夹心法原理进行定量分析，适用于检测血清、血浆及组织匀浆样本中的GLP-1含量。GLP-1是一种由肠道L细胞分泌的肠促胰岛素，在调节葡萄糖代谢、促进胰岛素分泌及抑制胃排空等生理过程中发挥关键作用，是研究糖尿病、肥胖及相关代谢疾病的重要靶点。该试剂盒检测范围为125 pg/ml至8000 pg/ml，通过预包被特异性抗体捕获样本中的目标蛋白，结合酶标二抗形成复合物后进行显色反应，最终通过标准曲线计算样本浓度。实验操作流程标准化，适用于科研领域中对GLP-1表达水平的定量分析，如糖尿病发病机制研究、代谢调控药物筛选、肠道激素功能探索等方向，可为体外实验提供稳定可靠的数据支持。试剂盒包含标准品、预包被板、检测抗体及显色底物等核心组分，需自备酶标仪等基础实验设备。

别名：

N/A

缩写：

GLP-1

种属：

Mus musculus (Mouse)

样本类型：

serum, plasma, tissue homogenates.

检测范围：

125 pg/ml-8000 pg/ml.

灵敏度：

31.25 pg/ml

反应时间：

1-5h

样本体积：

50-100ul

检测波长：

450 nm

研究领域：

Metabolism

测定原理：

quantitative

测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse GLP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	104
	Range %	98-110
1:2	Average %	95
	Range %	91-105
1:4	Average %	101
	Range %	94-108
1:8	Average %	93
	Range %	86-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

8000

2.397

2.334

2.365

2.173

4000

1.701

1.667

1.684

1.492

2000

1.162

1.098

1.130

0.938

1000

0.788

0.819

0.803

0.611

500

0.572

0.551

0.562

0.370

250

0.413

0.423

0.418

0.226

125

0.302

0.294

0.298

0.106

0

0.189

0.195

0.192

本试剂盒所含材料：

A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-mouse GLP-1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled GLP-1 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
An instruction manual

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本试剂盒不含材料：

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
50-300ul multi-channel micropipette
Pipette tips
Single-channel micropipette with different ranges
100ml and 500ml graduated cylinders
Deionized or distilled water
Timer
Test tubes for dilution

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数据处理：

ELISA操作视频及标曲绘制软件下载

货期：

3-5 working days

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.