Mouse interferon alpha-2(IFNA2) ELISA kit

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产品详情

产品描述：

本产品CSB-EL011038MO是基于双抗体夹心ELISA技术开发的定量检测试剂盒，专用于科研领域检测小鼠血清、血浆、组织匀浆及细胞裂解液中的干扰素α2（IFNA2）水平。IFNA2作为I型干扰素家族成员，在抗病毒免疫应答和免疫调节中发挥重要作用，其动态变化常被用于评估病毒感染模型、自身免疫性疾病或炎症反应机制的研究。试剂盒采用高特异性配对抗体，可在9.38-600 pg/mL线性范围内精准检测目标蛋白，最低检测限达9.38 pg/mL，满足微量样本分析需求。该产品适用于体外实验中IFNA2表达水平的动态监测，例如病毒学研究中宿主免疫应答评估、免疫调节机制探索或炎症相关疾病模型构建等科研场景。实验全程包含预包被板、标准品、检测抗体及显色系统，操作流程标准化，兼容常规酶标仪分析，为研究者提供稳定可靠的定量检测方案。
别名：

Ifna2Interferon alpha-2 ELISA Kit; IFN-alpha-2 ELISA Kit
缩写：

IFNA2
Uniprot No.：

P01573
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

9.38 pg/mL-600 pg/mL
灵敏度：

2.35 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFNA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-94
1:2	Average %	102
	Range %	98-107
1:4	Average %	84
	Range %	80-88
1:8	Average %	95
	Range %	95-99

回收率：

The recovery of mouse IFNA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-98
EDTA plasma (n=4)	98	94-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.