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  4. Mouse matrix metalloproteinase 1,MMP-1 ELISA kit

Mouse matrix metalloproteinase 1,MMP-1 ELISA kit

文献引用 (15) 评价 (1) 说明书下载
  • 中文名称：
    小鼠基质金属蛋白酶1(MMP-1)酶联免疫试剂盒
  • 货号：
    CSB-E07417m
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    小鼠基质金属蛋白酶1(MMP-1)酶联免疫试剂盒（CSB-E07417m）检测血清、血浆、组织培养上清液、组织匀浆样本中的MMP-1含量。MMP-1，也称为间质胶原酶和成纤维细胞胶原酶，是基质金属蛋白酶家族的一员。它参与细胞外基质的降解，在正常生理过程和疾病过程中发挥作用。MMP-1通过降解胶原蛋白和明胶等成分，影响细胞微环境，与肿瘤侵袭、转移和骨关节炎等疾病相关。研究MMP-1有助于开发针对相关疾病的治疗策略。试剂盒检测范围为47 pg/mL-3000 pg/mL，适用于体外实验研究，可支持细胞功能探索、疾病模型建立及药物作用机制分析等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Mmp1a ELISA Kit; McolAInterstitial collagenase A ELISA Kit; EC 3.4.24.7 ELISA Kit; Matrix metalloproteinase-1a ELISA Kit; MMP-1a ELISA Kit; Mcol-A ELISA Kit
  • 缩写：
    MMP-1
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    47 pg/mL-3000 pg/mL
  • 灵敏度：
    11.75 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse MMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:20 Average % 91  
    Range % 86-95  
    1:40 Average % 100  
    Range % 97-103  
    1:80 Average % 91  
    Range % 87-95  
    1:160 Average % 97  
    Range % 93-100  
  • 回收率：
    The recovery of mouse MMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 89-97  
    EDTA plasma (n=4) 96 90-98  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    3000 2.482 2.510 2.496 2.317  
    1500 1.855 1.786 1.821 1.642  
    750 1.246 1.232 1.239 1.060  
    375 0.748 0.774 0.761 0.582  
    187.5 0.495 0.456 0.476 0.297  
    93.75 0.322 0.339 0.331 0.152  
    46.88 0.253 0.242 0.248 0.069  
    0 0.180 0.178 0.179 　  
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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产品评价

平均分:
5.0分 - 1 个评价

样品类型：血清

样品信息：小鼠

稀释比：其他 1:10

产品评价： 我用CSB-E07417m检测小鼠血清MMP-1水平，其OD如图所示，试剂盒样本充足，使用说明简洁明了，操作容易上手，有助于实验进展，满意。

By 刘老师

相关产品

Mmp1a Proteins

靶点详情

  • 功能：
    Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. Able to degrade synthetic peptides and type I and II fibrillar collagen.
  • 基因功能参考文献：
    1. The difficulty to effectively maintain Mmp1a in the zymogen form may account for the tight control of Mmp1a expression and reduced expression in normal tissue as compared to inflammatory states or cancer. PMID: 24737602
    2. Stromal Mmp1a drives in vivo tumorigenesis. PMID: 23708660
    3. Matrix metalloproteinase Mmp-1a is not required for growth and fertility in mice and has a role in lung cancer progression by modulating inflammatory responses PMID: 23548910
  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    Peptidase M10A family
  • 数据库链接：

    KEGG: mmu:83995

    STRING: 10090.ENSMUSP00000034492

    UniGene: Mm.156952

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