Mouse mucin-5 subtype B,MUC5B ELISA Kit

产品详情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO小鼠粘蛋白5B（MUC5B）酶联免疫检测试剂盒（货号：CSB-E15067m），适用于定量检测血清、血浆、细胞培养上清及组织匀浆样本中的MUC5B蛋白。MUC5B是黏蛋白家族重要成员，广泛分布于呼吸道、消化道等黏膜组织，参与黏液屏障形成及病原体防御，其表达异常与慢性炎症性疾病及组织修复过程密切相关。CUSABIO试剂盒采用双抗体夹心法原理，通过高特异性抗体对目标蛋白进行捕获与检测，灵敏度达1.25 ng/mL，线性检测范围为1.25-80 ng/mL，可在标准曲线内精准定量样本浓度。该试剂盒适用于体外实验研究，如探究呼吸道疾病模型中黏液分泌调控机制、胃肠黏膜屏障功能评估、肿瘤微环境中黏蛋白表达分析等科研场景，为炎症性疾病、黏膜相关病理生理学研究提供可靠工具。操作流程包含标准品梯度稀释、样本预处理及酶标反应体系构建，配套提供优化缓冲液与显色底物，确保实验稳定性和重复性。
缩写：

MUC5B
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

1.25 ng/mL-80 ng/mL
灵敏度：

0.31 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse MUC5B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	81-94
1:2	Average %	96
	Range %	91-102
1:4	Average %	99
	Range %	93-105
1:8	Average %	92
	Range %	86-98

回收率：

The recovery of mouse MUC5B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-96
EDTA plasma (n=4)	98	90-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

平均分:

5.0分 - 2 个评价

样品类型：组织匀浆液肺组织匀浆上清

样品信息：小鼠

稀释比：没有稀释

产品评价：我通过使用CSB-E15067m检测了PR8病毒感染的小鼠肺组织匀浆上清，操作简单方便易上手，灵敏度非常高，检测结果十分精准

By 邓老师

样品类型：组织匀浆液肺组织匀浆上清

样品信息：小鼠

稀释比：没有稀释

产品评价：我通过使用CSB-E15067m检测了PR8病毒感染的小鼠肺组织匀浆上清，操作简单方便易上手，灵敏度非常高，检测结果十分精准

By 邓老师