SARS-CoV-2 N ELISA Kit
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中文名称:新型冠状病毒N蛋白 (SARS-CoV-2 N) 酶联免疫试剂盒
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货号:CSB-EL33251
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规格:96T/48T
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价格:¥3600/¥2500
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其他:
产品详情
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产品描述:CUSABIO新型冠状病毒核衣壳蛋白(SARS-CoV-2 N)定量检测试剂盒,采用双抗体夹心法原理,适用于科研领域对血清、血浆及拭子样本中N蛋白的精准检测。SARS-CoV-2 N蛋白是病毒核心结构蛋白,在病毒基因组包装及免疫逃逸中发挥关键作用,其定量检测可为病毒感染机制、宿主免疫应答研究提供重要数据支持。试剂盒检测灵敏度达3.12 ng/mL,线性范围为3.12-200 ng/mL,可覆盖多数科研场景下的样本浓度需求。该体系支持多类型样本检测,尤其适用于评估动物模型呼吸道拭子中的病毒载量、监测疫苗免疫后体液抗体水平变化,或用于病毒蛋白与宿主互作研究中的定量分析。本试剂盒经严格验证,包含预包被捕获抗体、生物素标记检测抗体及标准品等完整组分,操作流程标准化,可在4.5小时内完成检测。科研人员可通过该产品建立病毒感染的动态监测模型,或用于抗病毒药物筛选实验中靶蛋白表达水平的定量评估,为新冠肺炎相关基础研究提供可靠工具。
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别名:N; Nucleoprotein; N; Nucleocapsid protein; NC; Protein N
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缩写:SARS-CoV-2 N
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Uniprot No.:
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样本类型:serum, plasma, swabs
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检测范围:3.12 ng/mL-200 ng/mL
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灵敏度:0.78 ng/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Infectious Diseases
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean(ng/ml) 25.39 24.46 26.31 26.65 26.31 24.19 SD 0.027 0.025 0.031 0.041 0.035 0.037 CV(%) 5.7 5.46 6.33 8.26 7.13 8.01 -
标准曲线:These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected 200 3.228 3.104 3.166 3.032 100 2.045 2.169 2.107 1.973 50 1.001 0.931 0.966 0.832 25 0.469 0.507 0.488 0.354 12.5 0.268 0.276 0.272 0.138 6.25 0.196 0.204 0.200 0.066 3.12 0.168 0.161 0.165 0.031 0 0.136 0.132 0.134 -
数据处理:
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货期:3-5 working days
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靶点详情
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功能:Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host smad3.
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亚细胞定位:Virion. Host cytoplasm.
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蛋白家族:Betacoronavirus nucleocapsid protein family