Pig Brain-derived neurotrophic factor(BDNF) ELISA kit
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中文名称：
猪脑源性神经营养因子(BDNF)酶联免疫试剂盒
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货号：
CSB-EL002655PI
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规格：
96T/48T
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价格：
￥4200/￥3000
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其他：
产品详情
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产品描述：
CUSABIO猪脑源性神经营养因子（BDNF）酶联免疫检测试剂盒（货号：CSB-EL002655PI），采用双抗体夹心法定量分析血清、血浆及组织匀浆样本中的BDNF水平。BDNF是神经营养因子家族的重要成员，广泛参与神经细胞存活、突触可塑性调节及神经再生等生理过程，其表达水平与神经发育、学习记忆功能及相关病理机制研究密切相关。本试剂盒检测范围为9.375-600 ng/mL，可精准覆盖常见样本中BDNF的浓度梯度，适用于猪模型研究中神经退行性疾病机制探索、药物干预效果评估或应激状态下神经营养因子动态变化分析等科研场景。实验操作流程标准化，提供预包被96孔板及全套检测组分，兼容常规酶标仪进行吸光度读数，可高效完成批量样本检测，为神经科学、畜牧兽医及比较医学领域的实验研究提供可靠工具。
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别名：
BDNFBrain-derived neurotrophic factor ELISA kit; BDNF) [Cleaved into: BDNF precursor form ELISA kit; ProBDNF)] ELISA kit
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缩写：
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Uniprot No.：
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种属：
Sus scrofa (Pig)
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样本类型：
serum, plasma, tissue homogenates
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检测范围：
9.375 ng/mL-600 ng/mL
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灵敏度：
2.35 ng/mL
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反应时间：
1-5h
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样本体积：
50-100ul
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检测波长：
450 nm
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研究领域：
Cardiovascular
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测定原理：
quantitative
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测定方法：
Sandwich
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精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig BDNF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 96 | | |
| Range % | 91-102 | | |
| 1:2 | Average % | 102 | | |
| Range % | 97-107 | | |
| 1:4 | Average % | 109 | | |
| Range % | 105-113 | | |
| 1:8 | Average % | 103 | | |
| Range % | 96-107 | | |
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回收率：
| The recovery of pig BDNF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 102 | 98-105 | | |
| EDTA plasma (n=4) | 86 | 93-92 | | |
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标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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 | ng/ml | OD1 | OD2 | Average | Corrected | | | | 600 | 2.134 | 2.081 | 2.108 | 2.016 | | | | 300 | 1.537 | 1.623 | 1.580 | 1.488 | | | | 150 | 1.167 | 1.123 | 1.145 | 1.053 | | | | 75 | 0.769 | 0.774 | 0.772 | 0.680 | | | | 37.5 | 0.513 | 0.503 | 0.508 | 0.416 | | | | 18.75 | 0.353 | 0.344 | 0.349 | 0.257 | | | | 9.375 | 0.227 | 0.221 | 0.224 | 0.132 | | | | 0 | 0.091 | 0.093 | 0.092 | | | |
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数据处理：
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货期：
3-5 working days
靶点详情
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功能：
Important signaling molecule that activates signaling cascades downstream of NTRK2. During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.; Important signaling molecule that activates signaling cascades downstream of NTRK2. Activates signaling cascades via the heterodimeric receptor formed by NGFR and SORCS2. Signaling via NGFR and SORCS2 plays a role in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. Promotes neuronal growth cone collapse.
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基因功能参考文献：
- measurement of brain derived neurotrophic factor in serum brings a practical approach to study the effects of environmental enrichment on neurobiological changes in domestic animals PMID: 30007130
- Lf upregulated several canonical signaling pathways associated with neurodevelopment and cognition and influenced ~10 genes involved in the brain-derived neurotrophin factor (BDNF) signaling pathway. PMID: 25146846
- Taken together, these data indicate that recurrent tethering stress in sows over 4.5 years results in a loss of neurotrophic support by BDNF, mediated by an overactive neuroendocrine system. PMID: 22584108
- FiO2 used for resuscitation affects matrix metalloproteinases MMP-9 and MMP-2, caspase-3 and BDNF PMID: 21151608
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亚细胞定位：
Secreted.; [BDNF precursor form]: Secreted.
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蛋白家族：
NGF-beta family
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组织特异性：
Brain and central nervous system.
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数据库链接：
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