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  4. Pig Ghrelin (GHRL) ELISA kit

Pig Ghrelin (GHRL) ELISA kit

文献引用 (2) 说明书下载
  • 中文名称：
    猪生饥饿激素(GHRL)酶联免疫试剂盒
  • 货号：
    CSB-E09437p
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    猪生饥饿激素（GHRL）酶联免疫试剂盒（货号：CSB-E09437p）基于夹心法原理开发，可定量检测猪血清、血浆及组织匀浆样本中的GHRL蛋白，检测灵敏度范围为62.5-4000 pg/mL。GHRL作为调控食欲和能量代谢的关键胃肠肽类激素，其水平变化与摄食行为、脂肪代谢及内分泌调节密切相关，是研究动物能量平衡与代谢调控机制的重要靶点。CUSABIO试剂盒采用高特异性抗体对实现精准定量，操作流程兼容常规酶标仪分析，适用于科研场景中GHRL相关基础研究，例如探索营养干预、代谢性疾病模型或生理周期变化对GHRL表达的影响。试剂盒支持多种样本类型检测，组织匀浆检测前需经离心预处理以确保样本均一性，为研究者提供灵活的实验方案设计空间，满足能量代谢机制、内分泌调控通路等领域的科研需求。
  • 别名：
    GHRLAppetite-regulating hormone ELISA Kit; Growth hormone secretagogue ELISA Kit; Growth hormone-releasing peptide ELISA Kit; Motilin-related peptide) [Cleaved into: Ghrelin; Obestatin] ELISA Kit
  • 缩写：
    GHRL
  • Uniprot No.：
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    62.5 pg/mL-4000 pg/mL
  • 灵敏度：
    15.6 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig GHRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %93
    Range %89-97
    1:2Average %99
    Range %94-103
    1:4Average %106
    Range %100-114
    1:8Average %95
    Range %90-100
  • 回收率：
    The recovery of pig GHRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8480-88
    EDTA plasma (n=4)8884-93
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1OD2AverageCorrected
    40002.481 2.583 2.532 2.382
    20001.873 1.904 1.889 1.739
    10001.293 1.221 1.257 1.107
    5000.734 0.708 0.721 0.571
    2500.427 0.462 0.445 0.295
    1250.322 0.302 0.312 0.162
    62.50.250 0.259 0.255 0.105
    00.154 0.146 0.150
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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GHRL Proteins

靶点详情

  • 功能：
    Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.; Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility.
  • 基因功能参考文献：
    1. These expression data may indicate that factors other than ghrelin and leptin are more involved in the regulation of feed intake in pigs apparently acclimated to heat stress. PMID: 27264891
    2. slc30A8 colocalizes with ghrelin and motilin in the gastrointestinal tract of pigs PMID: 26388512
    3. Studied an alternative vascular approach to the modulation of gastric ghrelin levels.. PMID: 25294792
    4. Obestatin enhances proliferation and differentiation of preadipocytes promoting PPARgamma and C/EBPa expression, and inhibiting preadipocyte apoptosis by decreasing expression of Caspase-3, Caspase-7 and Caspase-9. PMID: 24534601
    5. The expression of ghrelin in the digestive tract of low birth weight and normal weight piglets is reported. PMID: 24237675
    6. Data suggest that a dietary factor (here, high levels of dietary copper) is able to up-regulate expression of ghrelin in the fundic gland of growing pigs; the diets tested in this study also increase feed intake and promote weight gain. PMID: 22911428
    7. Ghrelin immunolocalization in the gastrointestinal tract of pigs at different ages were studied and it was concluded that gastric ghrelin expression is not related merely to age but could also potentially be influenced by food intake. PMID: 22281862
    8. BsrI polymorphism at the ghrelin gene locus is potentially associated with carcass and meat quality traits. PMID: 21669497
    9. Plasma concentrations of ghrelin were greater before feeding and decreased after feeding in pigs fed once per day; there was no consistent pattern to ghrelin secretion in the ad libitum fed group. PMID: 20399063
    10. glucose output by primary porcine hepatocytes in suspension culture, after incubation with acylated ghrelin (AG), unacylated ghrelin (UAG), and hexarelin (HEX). PMID: 15536157
    11. Immunization against ghrelin was associated with increased antibody titers, decreased voluntary food consumption, and decreased body weight gain by pigs. PMID: 16793235
    12. ghrelin mRNA expression is up-regulated 10 days after weaning in the gastric fundus of piglets, coinciding with the increase of H(+)-K(+)-ATPase mRNA expression and activity PMID: 16920167
    13. growth hormone stimulated both ghrelin synthesis and secretion in the ovarian follicles PMID: 17951088
    14. Ghrelin requires activation of the NOS/NO route, and its subsequent GC/cGMP signal transduction pathway, as necessary steps to induce GH secretion from somatotrophs. PMID: 18208548
    15. Expression of ghrelin mRNA is affected by estrus in the hypothalamus, pituitary gland and ovary. PMID: 18295988
    16. circulating total plasma ghrelin concentrations in the pig appear to be influenced by chronic changes in energy balance rather than the feeding pattern per se. PMID: 18434347
    17. provides novel evidence that ghrelin has a modulatory effect in the ovary PMID: 19212011

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Motilin family
  • 数据库链接：

    KEGG: ssc:396728

    STRING: 9823.ENSSSCP00000012327

    UniGene: Ssc.440

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