Pig Interferon γ,IFN-γ ELISA Kit
-
中文名称：猪γ干扰素(IFN-γ)酶联免疫试剂盒
-
货号：CSB-E06794p-IS
-
规格：96T/48T
-
价格：￥4200/￥3000
-
其他：
产品详情
-
产品描述：猪γ干扰素(IFN-γ)酶联免疫试剂盒（CSB-E06794p-IS）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IFNG含量。IFNG即干扰素-γ，是一种具有多种生物学功能的细胞因子。它在免疫调节中发挥核心作用，可增强巨噬细胞活性、促进Th1细胞分化。研究机制上，通过与受体结合激活信号通路，调节免疫相关基因表达，在抗感染、抗肿瘤及自身免疫病研究中备受关注。试剂盒检测范围为6.25 pg/mL-400 pg/mL，灵敏度为3.12 pg/mL。该产品适用于动物免疫学、传染病模型研究、疫苗效力评估等科研领域，可定量分析猪感染性疾病（如猪繁殖与呼吸综合征、猪瘟等）或炎症反应过程中IFN-γ的动态变化，为研究宿主免疫应答机制、病原体与宿主相互作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
-
别名：IFNGInterferon gamma ELISA Kit; IFN-gamma ELISA Kit
-
缩写：
-
Uniprot No.：
-
种属：Sus scrofa (Pig)
-
样本类型：serum, plasma, tissue homogenates
-
检测范围：6.25 pg/mL-400 pg/mL
-
灵敏度：3.12 pg/mL
-
反应时间：1-5h
-
样本体积：50-100ul
-
检测波长：450 nm
-
研究领域：Immunology
-
测定原理：quantitative
-
测定方法：Sandwich
-
精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
-
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of pig IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
101
Range %
94-108
1:2
Average %
92
Range %
85-99
1:4
Average %
96
Range %
91-101
1:8
Average %
93
Range %
86-99
-
回收率：
The recovery of pig IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
90
86-96
EDTA plasma (n=4)
92
87-98
-
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
400
2.687
2.634
2.660
2.547
200
1.913
1.967
1.940
1.827
100
1.233
1.188
1.211
1.097
50
0.659
0.629
0.644
0.531
25
0.358
0.321
0.340
0.226
12.5
0.223
0.233
0.228
0.115
6.25
0.176
0.184
0.180
0.067
0
0.115
0.111
0.113
-
数据处理：
-
货期：3-5 working days
产品评价
相关产品
靶点详情
-
功能：Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL. Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation.
-
基因功能参考文献：
- The regulatory effect of IFN-gamma on CYP3A29 expression is mediated via PXR. PMID: 25413352
- Mapping of QTL for mycoplasma and tetanus antibodies and IFNgamma. PMID: 20567833
- Data show taht the expression of PoIFN-gamma in insect cells was confirmed by Western Blot, indirect immunofluorescence assay and indirect ELISA. PMID: 17577980
- IFNgamma production from the frozen peripheral blood mononuclear cells was significantly higher than that from the fresh ones. PMID: 19766617
- Translational control of IL-18 expression by its 5'-UTR limits production of IL-18, resulting in restricted expression of mRNA and protein IFN-gamma in this model of crescentic glomerulonephritis(GN). Might amplify CD8+-mediated macrophage-dependent GN. PMID: 12787406
- selective changes in immature crypt cells induced by IFN-gamma bound to extracellular matrix could contribute to inappropriate responsiveness to commensal bacteria in inflammatory bowel diseases PMID: 15894119
- A functional single nucleotide polymorphism is reported in the coding region of IFN-gamma cDNA that markedly reduces antiviral activity of the IFN-gamma protein. PMID: 17416419
- The expression of IFN-gamma in recombiannt Lactococcus lactis as a tool for investigating downregulation of allergic predisposition of pigs to experimental food allergy is reported. PMID: 18164876
收起更多
-
亚细胞定位：Secreted.
-
蛋白家族：Type II (or gamma) interferon family
-
组织特异性：Released primarily from activated T lymphocytes.
-
数据库链接：
