Pig Keratinocyte Growth Factor,KGF ELISA Kit

产品详情

文献引用 (1) 说明书下载

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO猪角化细胞生长因子（KGF）酶联免疫检测试剂盒（货号CSB-E06800p），采用双抗体夹心法定量分析血清或血浆样本中KGF蛋白含量。KGF是一种由间充质细胞分泌的多肽生长因子，特异性作用于上皮细胞，在皮肤组织修复、黏膜再生及毛囊发育等生理过程中发挥关键调控作用。试剂盒检测浓度区间覆盖3.12-200 pg/mL，通过预包被高亲和力捕获抗体与标记检测抗体的协同作用实现精准定量，适用于猪模型中与表皮损伤修复、炎症反应或毛发再生相关的分子机制研究。实验操作需50-100μL样本量，标准品与样本均设复孔检测，配套提供标准品、检测抗体及显色底物等完整组分。该试剂盒可广泛应用于农业科研中猪皮肤疾病模型构建、药物干预效果评估或生物工程材料促愈活性分析等基础研究场景，为探索KGF在猪生理病理过程中的作用提供可靠工具。
别名：

FGF7Fibroblast growth factor 7 ELISA kit; FGF-7 ELISA kit; Heparin-binding growth factor 7 ELISA kit; HBGF-7 ELISA kit; Keratinocyte growth factor ELISA kit; KGF ELISA kit
缩写：

FGF7
Uniprot No.：

Q9N198
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma
检测范围：

3.12 pg/mL-200pg/mL
灵敏度：

0.78 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig KGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-101
1:2	Average %	88
	Range %	84-92
1:4	Average %	90
	Range %	85-98
1:8	Average %	106
	Range %	101-114

回收率：

The recovery of pig KGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	84	80-88

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation.
亚细胞定位：

Secreted.
蛋白家族：

Heparin-binding growth factors family
数据库链接：

STRING: 9823.ENSSSCP00000025433

UniGene: Ssc.15923