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  4. Pig basic fibroblast growth factor,bFGF ELISA Kit

Pig basic fibroblast growth factor,bFGF ELISA Kit

文献引用 (3) 说明书下载
  • 中文名称：
    猪碱性成纤维细胞生长因子(bFGF)酶联免疫试剂盒
  • 货号：
    CSB-E12841p
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO猪碱性成纤维细胞生长因子（bFGF）酶联免疫检测试剂盒（货号：CSB-E12841p），采用双抗体夹心法原理实现定量分析，适用于检测血清、血浆及组织匀浆样本中bFGF的蛋白表达水平。bFGF是一种广泛参与细胞增殖、分化和血管生成等生理过程的多功能生长因子，在组织修复、胚胎发育及肿瘤微环境调控等科研领域具有重要研究价值。本试剂盒检测灵敏度高，线性范围为4.7-300 pg/mL，可精准捕捉低浓度样本的信号变化。预包被的特异性抗体可确保检测的高特异性，配套标准品与显色系统经过严格验证，支持用户通过酶标仪在450 nm波长下快速读取数据。该产品适用于体外研究，如探究猪模型中创伤愈合机制、干细胞培养条件优化或疾病相关生长因子通路分析，为心血管疾病、再生医学及肿瘤生物学等基础研究提供可靠的实验工具。操作流程简便，兼容常规实验室设备，实验结果重复性强，满足科研工作者对bFGF蛋白定量检测的高标准需求。
  • 别名：
    FGF2Fibroblast growth factor 2 ELISA Kit; FGF-2 ELISA Kit; Basic fibroblast growth factor ELISA Kit; bFGF ELISA Kit; Heparin-binding growth factor 2 ELISA Kit; HBGF-2 ELISA Kit; Prostatic growth factor ELISA Kit; Fragments ELISA Kit
  • 缩写：
    FGF2
  • Uniprot No.：
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    4.7 pg/mL-300 pg/mL
  • 灵敏度：
    1.2 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig bFGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 88  
    Range % 80-100  
    1:2 Average % 97  
    Range % 91-105  
    1:4 Average % 94  
    Range % 90-110  
    1:8 Average % 95  
    Range % 82-105  
  • 回收率：
    The recovery of pig bFGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 85-104  
    EDTA plasma (n=4) 98 92-106  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    300 2.175 2.157 2.166 1.966  
    150 1.582 1.612 1.597 1.397  
    75 1.202 1.134 1.168 0.968  
    37.5 0.628 0.603 0.616 0.416  
    18.75 0.377 0.362 0.370 0.170  
    9.4 0.296 0.301 0.299 0.099  
    4.7 0.265 0.256 0.261 0.061  
    0 0.196 0.204 0.200 　  
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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FGF2 Proteins

靶点详情

  • 功能：
    Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4. Also acts as an integrin ligand which is required for FGF2 signaling. Binds to integrin ITGAV:ITGB3. Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Can induce angiogenesis. Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation.
  • 亚细胞定位：
    Secreted. Nucleus.
  • 蛋白家族：
    Heparin-binding growth factors family
  • 数据库链接：

    STRING: 10141.ENSCPOP00000004847

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