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  4. Pig brain natriuretic peptide,BNP ELISA Kit

Pig brain natriuretic peptide,BNP ELISA Kit

文献引用 (1) 说明书下载
  • 中文名称：
    猪脑钠素/脑钠尿肽(BNP)酶联免疫试剂盒
  • 货号：
    CSB-E06807p
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO猪脑钠素/脑钠尿肽（BNP）酶联免疫检测试剂盒（货号CSB-E06807p），采用双抗体夹心法定量检测猪血清、血浆或组织匀浆样本中的BNP浓度，检测灵敏度达31.25 pg/mL，线性范围为31.25-2000 pg/mL。BNP是由心室肌细胞分泌的神经激素，其水平变化与心脏功能调节、体液平衡及心血管疾病进程密切相关。本试剂盒通过高特异性抗体实现对BNP的精准捕获与定量分析，适用于心血管疾病基础研究、动物疾病模型评估、药效学实验中生物标志物动态监测等科研场景。实验操作全程无需特殊设备，常规酶标仪即可完成读数，配套标准品及预包被板可实现快速上样检测。该试剂盒为科研工作者提供稳定可靠的BNP检测方案，助力心脏功能调控机制探索及相关病理生理研究。
  • 别名：
    NPPBNatriuretic peptides B ELISA kit; Gamma-brain natriuretic peptide) [Cleaved into: Brain natriuretic peptide 32 ELISA kit; BNP-32); Brain natriuretic peptide 26 ELISA kit; BNP-26)] ELISA kit
  • 缩写：
    BNP
  • Uniprot No.：
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.8 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig BNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 90  
    Range % 86-95  
    1:2 Average % 94  
    Range % 89-98  
    1:4 Average % 85  
    Range % 80-93  
    1:8 Average % 98  
    Range % 92-102  
  • 回收率：
    The recovery of pig BNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 91-102  
    EDTA plasma (n=4) 88 84-92  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    2000 2.551 2.501 2.526 2.392  
    1000 2.003 1.961 1.982 1.848  
    500 1.271 1.212 1.242 1.108  
    250 0.742 0.719 0.731 0.597  
    125 0.407 0.435 0.421 0.287  
    62.5 0.326 0.303 0.315 0.181  
    31.25 0.227 0.214 0.221 0.087  
    0 0.139 0.128 0.134 　  
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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NPPB Proteins

靶点详情

  • 功能：
    Cardiac hormone that plays a key role in mediating cardio-renal homeostasis. May also function as a paracrine antifibrotic factor in the heart. Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses. Involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Binds the clearance receptor NPR3.; Plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. In vivo, induces vasodilation.; May affect cardio-renal homeostasis. Able to promote the production of cGMP although its potency is very low compared to brain natriuretic peptide 32.
  • 基因功能参考文献：
    1. CNP and BNP serve as oocyte maturation inhibitor to inhibit oocyte maturation in mammals PMID: 24912131
    2. ANP and BNP gene expression differs considerably between cardiac chambers in the first 72 hours of life in healthy piglets, resembling the transition from fetal to neonate circulation. The cardiac gene expression does not explain plasma concentrations. PMID: 23539511
    3. Up-regulation of BNP and a reduction in a ratio of sarcoplasmic reticulum calcium ATPase 2a in failing myocardium are observed in a porcine model of tachycardia. PMID: 20880599
    4. serum levels predict mortality in chest pain patients in Argentina PMID: 21958326
    5. Data show that all five molecules, BNP, ICAM-1, TNF-alpha, VCAM-1 and IL-6, quickly and reliably signaled adverse interactions. PMID: 21471537
    6. identification and characterization of a novel alternatively spliced variant of porcine and human NPPB resulting from exon 2 skipping PMID: 20660094
    7. Arial natriuretic factor (ANF) and N-terminal proANF may be better suited than brain natriuretic peptide as markers of cardiac preload during the development and treatment of acute heart failure. PMID: 19361150
    8. Regional differences in BNP and CNP expression were observed in myocardium with sustained left ventricular dyssynchronous contraction. PMID: 19540894

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  • 亚细胞定位：
    Secreted.; [Brain natriuretic peptide 32]: Secreted.
  • 蛋白家族：
    Natriuretic peptide family
  • 组织特异性：
    Heart atrium.; [Brain natriuretic peptide 32]: Brain (at protein level).; [Brain natriuretic peptide 26]: Brain (at protein level).
  • 数据库链接：

    KEGG: ssc:396844

    STRING: 9823.ENSSSCP00000003722

    UniGene: Ssc.629

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