Pig calcitonin gene related peptide(CGRP)ELISA Kit

产品详情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO猪降钙素基因相关肽（CGRP）酶联免疫检测试剂盒（货号CSB-E16438p），采用双抗体夹心法定量检测血清、血浆及组织匀浆样本中的CGRP含量。CGRP是一种广泛分布于神经系统的神经肽，具有调节血管张力、参与痛觉传导及炎症反应等重要生理功能，其水平变化与神经源性炎症、心血管疾病等病理过程密切相关。试剂盒检测灵敏度高，标准曲线范围为31.25-2000 pg/mL，可精准覆盖常见生物样本中CGRP的浓度区间。试剂盒包含预包被特异性抗体的酶标板、标准品及检测所需全套试剂组分，操作流程标准化，适用于神经生物学、心血管研究、疼痛机制探索等科研领域，尤其适合用于建立动物模型（如偏头痛或高血压模型）后评估CGRP动态变化，或通过组织样本分析特定器官中CGRP的表达水平。本产品仅限科学研究使用，建议实验前通过预实验优化样本稀释倍数以确保检测值位于标准曲线线性范围内。
缩写：

CGRP
Uniprot No.：

A6P7L6
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.81 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig CGRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	84-94
1:2	Average %	102
	Range %	97-107
1:4	Average %	99
	Range %	96-102
1:8	Average %	98
	Range %	94-102

回收率：

The recovery of pig CGRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	90-96
EDTA plasma (n=4)	97	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.